Elucidating the role of bovine GnRHR on semen quality traits among crossbred bull

SUSHIL KUMAR; INDRAJIT GANGULY; RAJIB DEB; UMESH SINGH; DILIP KUMAR MANDAL; SANDEEP MANN; RANI SINGH; MAHESH KUMAR; ARJAVA SHARMA

doi:10.56093/ijans.v85i12.54384

Authors

SUSHIL KUMAR Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
INDRAJIT GANGULY Senior Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
RAJIB DEB Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
UMESH SINGH Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
DILIP KUMAR MANDAL Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
SANDEEP MANN Senior Research Fellow, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
RANI SINGH Research Associate, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
MAHESH KUMAR Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
ARJAVA SHARMA Directo, Molecular Genetics Laboratory, Animal Genetics & Breeding Division, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India

https://doi.org/10.56093/ijans.v85i12.54384

Keywords:

Frieswal, GnRHR, G286A, Semen, T340C

Abstract

Gonadotropin-releasing hormone receptor (GnRHR) gene plays an important role in endocrine regulation as well as gonadal development. Two SNPs (G286A and T340C) of GnRHR were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and 3 combinations of these SNPs were observed. The single SNP and their genetic effects on semen quality traits were evaluated and a significant association with semen volume and concentration was found in T340C SNP. The mean semen volume and concentration of individuals with genotype TT showed significantly higher sperm concentration and lower semen volume compared to bulls with heterozygote TC genotype. The results of combined genotypes analysis of two SNPs showed that GGTC had the significantly highest semen volume and lowest concentration compare to the others.

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