Elucidating the role of bovine GnRHR on semen quality traits among crossbred bull


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Authors

  • SUSHIL KUMAR Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • INDRAJIT GANGULY Senior Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • RAJIB DEB Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • UMESH SINGH Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • DILIP KUMAR MANDAL Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • SANDEEP MANN Senior Research Fellow, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • RANI SINGH Research Associate, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • MAHESH KUMAR Principal Scientist, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India
  • ARJAVA SHARMA Directo, Molecular Genetics Laboratory, Animal Genetics & Breeding Division, ICAR- Central Institute for Research on Cattle, Meerut, Uttar Pradesh 250 001 India

https://doi.org/10.56093/ijans.v85i12.54384

Keywords:

Frieswal, GnRHR, G286A, Semen, T340C

Abstract

Gonadotropin-releasing hormone receptor (GnRHR) gene plays an important role in endocrine regulation as well as gonadal development. Two SNPs (G286A and T340C) of GnRHR were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and 3 combinations of these SNPs were observed. The single SNP and their genetic effects on semen quality traits were evaluated and a significant association with semen volume and concentration was found in T340C SNP. The mean semen volume and concentration of individuals with genotype TT showed significantly higher sperm concentration and lower semen volume compared to bulls with heterozygote TC genotype. The results of combined genotypes analysis of two SNPs showed that GGTC had the significantly highest semen volume and lowest concentration compare to the others.

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References

Albertson A J, Navratil A, Mignot M, Dufourny L, Cherrington B and Skinner D C. 2008. Immunoreactive GnRH type I receptors in the mouse and sheep brain. Journal of Chemical Neuroanatomy 35: 326–33. DOI: https://doi.org/10.1016/j.jchemneu.2008.03.004

Deb R, Kumar S, Singh U, Tyagi S, Mandal D K, Sengar G, Singh R, Kumar M and Sharma A. 2013. Evaluation of three bovine Y specific microsatellite loci as an alternative biomarkers for semen quality traits in crossbred bull. Animal Reproduction Science 142: 121–25. DOI: https://doi.org/10.1016/j.anireprosci.2013.09.015

Ferreira G, Silveira L, Barbosa T E, Latronico AC. 2010. Genetics basis for GnRH-dependent pubertal disorders in humans. Molecular Cell Endocrinology 324:30–38. DOI: https://doi.org/10.1016/j.mce.2010.02.023

Ganguly I, Gaur G K, Kumar S, Mandal D K, Kumar M, Singh U, Kumar S and Sharma A . 2013. Differential expression of protamine 1 & 2 genes in mature spermatozoa of normal and motility impaired semen producing Frieswal (HF × Sahiwal) bulls. Research in Veterinary Science 94: 256–62. DOI: https://doi.org/10.1016/j.rvsc.2012.09.001

Kumar S, Deb R, Singh U, Ganguly I, Mandal D K, Tyagi S, Kumar M, Sengar G, Sharma S, Singh R, Singh R. 2015. Bovine Circadian Locomotor Output Cycles Kaput (CLOCK) and Clusterin (CLU) mRNA quantitation in ejaculated crossbred bull spermatozoa. Reproduction in Domestic Animals DOI: 10.1111/rda.12522. DOI: https://doi.org/10.1111/rda.12522

Leanos-Miranda A, Janovick J A and Conn P M. 2002. Receptormisrouting: an unexpectedly prevalent and rescuable etiology in gonadotropin-releasing hormone receptor-mediated hypogonadotropichypogonadism. Journal of Clinical Endocrinology and Metabolism 87: 4825–28. DOI: https://doi.org/10.1210/jc.2002-020961

Lin C L, Ponsuksili S, Tholen E, Jennen D G, Schellander K, Wimmers K. 2006. Candidate gene markers for spermquality and fertility of boar. Animal Reproduction Science 92: 349– 63. DOI: https://doi.org/10.1016/j.anireprosci.2005.05.023

Milazzotto M P, Rahal P, Nichi M., MirandaNeto T, Teixeira L A, Ferraz J B S, Eler J P, Campagnari F, Garcia J F. 2008. New molecular variants of hypothalamus–pituitary–gonad axis genes and their association with early puberty phenotype in Bos taurus indicus (Nellore). Livestock Science 114: 274–79. DOI: https://doi.org/10.1016/j.livsci.2007.05.006

Schubert U, Anton LC, Gibbs J, Norbury C C, Yewdell J W and Bennink J R .2000. Rapid degradation of a large fraction of newly synthesized proteins by proteasomes. Nature 404: 770– 74. DOI: https://doi.org/10.1038/35008096

Yang W C, Li S J, Xie Y H, Tang K Q, Hua G H, Zhang C Y, Yang L G. 2011. Two novel SNPs of the type I gonadotropin releasing hormone receptor gene and their associations with superovulation traits in Chinese Holstein cows. Livestock Science 136: 164–68. DOI: https://doi.org/10.1016/j.livsci.2010.08.012

Zheng X, Ju Z, Wang J, Li Q, Huang J, Zhang A, Zhong J, Wang C. 2011. Single nucleotide polymorphisms, haplotypes and combined genotypes of LAP3 gene in bovine and their association with milk production traits. Molecular Biology Reports 38: 4053–61. DOI: https://doi.org/10.1007/s11033-010-0524-1

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Submitted

2015-12-18

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2015-12-18

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How to Cite

KUMAR, S., GANGULY, I., DEB, R., SINGH, U., MANDAL, D. K., MANN, S., SINGH, R., KUMAR, M., & SHARMA, A. (2015). Elucidating the role of bovine GnRHR on semen quality traits among crossbred bull. The Indian Journal of Animal Sciences, 85(12), 1314–1317. https://doi.org/10.56093/ijans.v85i12.54384
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