Leptin gene polymorphism in association with lactation milk yield in Murrah buffaloes

V JAMUNA; A K GUPTA; A K CHAKRAVARTY; AVTAR SINGH; C S PATIL; MANOJ KUMAR; VIKAS VOHRA

doi:10.56093/ijans.v86i1.55039

Authors

V JAMUNA ICAR - National Dairy Research Institute, Karnal, Haryana 132 001 India
A K GUPTA ICAR - National Dairy Research Institute, Karnal, Haryana 132 001 India
A K CHAKRAVARTY ICAR - National Dairy Research Institute, Karnal, Haryana 132 001 India
AVTAR SINGH ICAR - National Dairy Research Institute, Karnal, Haryana 132 001 India
C S PATIL LUVAS, Hisar
MANOJ KUMAR ICAR - National Dairy Research Institute, Karnal, Haryana 132 001 India
VIKAS VOHRA NBAGR, Karnal

https://doi.org/10.56093/ijans.v86i1.55039

Keywords:

Lactation milk yield, Leptin gene, Murrah buffaloes PCR-RFLP, Polymorphism

Abstract

PCR amplification of exon-2 of leptin gene yielded an amplified product of 210 bp in Murrah buffaloes. RFLP of leptin gene done with Aci1 restriction enzyme revealed 3 types of genotypes CC, TC and TT in Murrah buffaloes of NDRI herd with a frequency of 0.26 , 0.73 and 0.013% respectively. Results suggested that CC genotype of leptin gene in Murrah buffaloes is significantly associated with 305 days milk yield. Overall study suggested the scope of considering genetic information of leptin gene in breeding and management strategies for the improvement of Murrah buffaloes of NDRI herd.

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