Effect of intrauterine Allium sativum extract on recovery and conception rate in dairy cows with subclinical endometritis

ATILLA YILDIZ

doi:10.56093/ijans.v86i2.55791

Authors

ATILLA YILDIZ Sivrice Vocational School, University of Firat, 23119 Elazig, Turkey

https://doi.org/10.56093/ijans.v86i2.55791

Keywords:

Cow, Garlic extract, Subclinical endometritis

Abstract

The current study was designed to investigate the therapeutic effectiveness of Allium sativum (garlic) extract infusion on recovery and conception rate in cows with subclinical endometritis. Cows (42) with subclinical endometritis were randomly divided into 2 equal groups. In treatment group (group T), cows received a single intrauterine administration of 10 ml garlic extract mixed with 40 ml saline. In the cows of control group (group C), no intrauterine treatment was administered. Samples were collected again from the all animals of both groups on day 14 after therapy and the same laboratory tests were repeated. The cows were artificially inseminated at the following estrus. 10 (23.8 %) out of 42 cows had subclinical endometritis without bacteria in the uterus; however majority of cows with subclinical endometritis were positive for bacterial infection (76.2%). Bacterial load in group T was significantly lower as compared to both pre-treatment and group C. The cure rate for group T and C was 100.0 and 19.0%, respectively. The conception rates in groups T and C were 52.4 and 14.3%, respectively. Our results indicated that the intrauterine use of garlic extract can decrease endometrial inflammation and bacterial load, and consequently an increase in the conception rate.

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References

Andleeb S, Tahir M, Khalid M, Awan U A, Riaz N and Ali S. 2014. Antibacterial and antioxidant activities of traditional herbs and honey against fish associated bacterial pathogens. Pakistan Journal of Zoology 46 (4): 933–40.

Barlund C S, Carruthers T D, Waldner C L and Palmer C W. 2008. A comparison of diagnostic techniques for postpartum endometritis in dairy cattle. Theriogenology 69 (6): 714–23. DOI: https://doi.org/10.1016/j.theriogenology.2007.12.005

Carneiro L C, Ferreira A F, Padua M, Saut J P, dos Santos R M and Ferraudo A S. 2014. Incidence of subclinical endometritis and its effects on reproductive performance of crossbred dairy cows. Tropical Animal Health Production 46 (8): 1435–39. DOI: https://doi.org/10.1007/s11250-014-0661-y

Corzo-Martinez M, Corzo N and Villamiel M. 2007. Biological properties of onions and garlic. Trends in Food Science and Technology 18 (12): 609–25. DOI: https://doi.org/10.1016/j.tifs.2007.07.011

Cowan S T and Steel K J. 1973. Manual for the Identification of Medical Bacteria. 2nd edn. University Press. Cambridge.

Deresse D. 2010. Antibacterial Effect of Garlic (Allium sativum) on Staphylococcu aureus: An in vitro Study. Asian Journal of Medical Sciences 2 (2): 62–65.

Eja M E, Arikpo G E, Enyi-Idoh K H and Ikpeme E M. 2011. An evaluation of the antimicrobial synergy of Garlic (Allium sativum) and Utazi (Gongronema latifolium) on Escherichia coli and Staphylococcus aureus. Malaysian Journal of Microbiology 7 (1): 49–53.

Eja M E, Asikong B E, Abriba C, Arikpo G E, Anwan E E and Enyi-Idoh KH. 2007. A comparative assessment of the antimicrobial effects of garlic (Allium sativum) and antibiotics on diarrheagenic organisms. Southeast Asian Journal of Tropical Medicine and Public Health 38 (2): 343–48.

Feldberg R S, Chang S C, Kotik A N, Nadler M, Neuwirth Z, Sundstrom D C and Thompson N H. 1988. In vitro mechanism of inhibition of bacterial growth by allicin. Antimicrobial Agents and Chemotherapy 32 (12): 1763–68. DOI: https://doi.org/10.1128/AAC.32.12.1763

Gani M O, Amin M M, Alam M G S, Kayesh M E H, Karim M R, Samad M A and Islam M R. 2008. Bacterial flora associated with repeat breeding and uterine infections in dairy cows. Bangladesh Journal of Veterinary Medicine 6 (1): 79–86. DOI: https://doi.org/10.3329/bjvm.v6i1.1342

Gilbert R O, Shin S T, Guard C L, Erb H N and Frajblat M. 2005. Prevalence of endometritis and its effects on reproductive performance of dairy cows. Theriogenology 64 (9): 1879–88. DOI: https://doi.org/10.1016/j.theriogenology.2005.04.022

Guo J J, Kuo C M, Chuang Y C, Hong J W, Chou R L and Chen T I. 2012. The effects of garlic-supplemented diets on antibacterial activity against Streptococcus iniae and on growth in orange-spotted grouper, Epinephelus coioides. Aquaculture 364–365: 33–38. DOI: https://doi.org/10.1016/j.aquaculture.2012.07.023

Hoedemaker M. 1998. Postpartal pathological vaginal discharge: to treat or not to treat? Reproduction Domestic Animal 33 (3– 4): 141–43. DOI: https://doi.org/10.1111/j.1439-0531.1998.tb01332.x

Ikpeme E M, Alobi N O, Enyi-Idoh K H, Okoi A I, Etim K D and Eja M E. 2013. The phytochemical and antimicrobial studies of Ficus exasperata and Cida acuta on Staphylococcus aureus and Escherichia coli. Central European Journal of Experimental Biology 2 (2): 1–4.

Jabar M A and Al-Mossawi A. 2007. Susceptibility of some multiple resistant bacterial to garlic extract. African Journal of Biotechnology 6 (6): 771–76.

Joe M M, Jayachitra J and Vijayapriya M. 2009. Antimicrobial activity of some common spices against certain human pathogens. Journal of Medicinal Plants Research 3 (11): 1134–36.

Kaufmann T B, Drillich M, Tenhagen B A, Forderung D and Heuwieser W. 2009. Prevalence of bovine subclinical endometritis 4 h after insemination and its effects on first service conception rate. Theriogenology 71 (2): 385–91. DOI: https://doi.org/10.1016/j.theriogenology.2008.08.005

Madoz L V, Uliodori G I, Jaureguiberry M J, Plöntzke M, Drillich J and de la Sota R L. 2013. The relationship between endometrial cytology during estrous cycle and cutoff points for the diagnosis of subclinical endometritis in grazing dairy cows. Journal of Dairy Science 96 (7): 4333–39. DOI: https://doi.org/10.3168/jds.2012-6269

Meziane R, Niar A, Smadi M A, Mamache B and Meziane T. 2013. A clinical study of metritis in dairy cows in the region of Batna (east of Algeria) and their treatments using different therapeutic protocols. Veterinary World 6 (1): 45–48. DOI: https://doi.org/10.5455/vetworld.2013.45-48

Moges N and Jebar A. 2012. Prevalence of subclinical endometritis and its effect on pregnancy in crossbred dairy cows in Gondar. Journal of Reproduction and Infertility 3 (2): 26–31.

Mohammadzadeh F, Dolatian M, Jorjani M, Majd H A and Borumandnia N. 2014. Comparing the therapeutic effects of garlic tablet and oral metronidazole on bacterial vaginosis: A randomized controlled clinical trial. Iranian Red Crescent Medical Journal 16 (7): e19118. DOI: https://doi.org/10.5812/ircmj.19118

Plöntzke J, Madoz L V, de la Sota R L, Drillich M and Heuwieser W. 2010. Subclinical endometritis and its impact on reproductive performance in grazing dairy cattle in Argentina. Animal Reproduction Science 122 (1–2): 52–57. DOI: https://doi.org/10.1016/j.anireprosci.2010.07.006

Prasad J K, Saxena M S and Prasad S. 2006. Comparative efficacy of immunomodulators and sensitive antibiotics on endometrial histopathology in endometritis in crossbred cows. Indian Journal of Animal Reproduction 27 (2): 45–53.

Rahi S, Gupta H P, Prasad S and Baithalu R K. 2013. Phytotherapy for endometritis and subsequent conception rate in repeat breeding crossbred cows. Indian Journal of Animal Reproduction 34 (1): 9–12.

Ricci A, Gallo S, Molinaro F, Dondo A, Zoppi S and Vincenti L. 2015. Evaluation of subclinical endometritis and consequences on fertility in Piedmontese beef cows. Reproduction Domestic Animal 50 (1): 142–48. DOI: https://doi.org/10.1111/rda.12465

Rose P, Whiteman M, Moore P K and Zhun Zhu Y. 2005. Bioactive Salk(en)yl cysteine sulfoxide metabolites in the genus Allium: the chemistry of potenial therapeutic agents. Natural Product Reports 22 (3): 351–68. DOI: https://doi.org/10.1039/b417639c

Sarkar P, Kumar H, Rawat M, Varshney V P, Goswami T K, Yadav M C and Srivastava S K. 2006. Effect of administration of garlic extract and PGF2ß on hormonal changes and recovery in endometritis cows. Asian Australasian Journal of Animal Sciences 19 (7): 964 –69. DOI: https://doi.org/10.5713/ajas.2006.964

Schäfer G and Kaschula C H. 2014. The immunomodulation and anti-inflammatory effects of garlic organosulfur compounds in cancer chemoprevention. Anti-Cancer Agents in Medicinal Chemistry 14 (2): 233–40. DOI: https://doi.org/10.2174/18715206113136660370

Sheldon I M, Price S B, Cronin J, Gilbert R O and Gadsby J E. 2009. Mechanisms of infertility associated with clinical and subclinical endometritis in high producing dairy cattle. Reproduction in Domestic Animals 44 (Suppl. 3): 1–9. DOI: https://doi.org/10.1111/j.1439-0531.2009.01465.x

Tattelman E. 2005. Health effects of garlic. American Family Physician 72 (01): 103–06.