Genetic polymorphism in Jakhrana goats and their comparison with other Indian goat breeds through RAPD
358 / 94
Keywords:
Genetic distance, Genetic similarity, Jakhrana, Polymorphism, RAPDAbstract
RAPD markers were used to estimate the genetic variability of Jakhrana breed with other extant goat breeds, and to evaluate genetic relationship between them. Adult females, 15 from each breed (Jakhrana, Barbari, Sirohi, Black Bengaland Jamunapari), were randomly selected. Polymorphic random primers OPM-12, OPM-18, OPM- 14, OPA-01, OPM-05, OPP-08, OPP-02 were used for analysis. The estimates were based on band sharing proportion and based on the band frequency. When pooled over the primers, based on band sharing, Black Bengal (0.890) showed minimum while Jakhrana showed maximum (0.994) within-breed genetic similarity. Similar trend was observed based on band frequency where Black Bengal (0.800) showed minimum and Jakhrana showed maximum (0.890) within breed genetic similarity. For between-breed similarities, based on band sharing, genetic similarity was observed minimum between Jamunapari and Jakhrana (0.846) while maximum was observed between Sirohi and Jakhrana (0.994), based on band frequency, the minimum was observed between Jamunapari and Sirohi (0.985), while Black Bengal and Barbari showed maximum genetic similarity of 0.999. These results can be utilized to upgrade and improve the breeds which are genetically close through a common breed improvement program.
Downloads
References
Anbarasan K, Sharma A K, Singh R K, Deb S M and Sharma D. 2001. Genetic differentiation among goats using randomly amplified polymorphic DNA (RAPD) markers. Journal of Applied Animal Research 20: 83–88. DOI: https://doi.org/10.1080/09712119.2001.9706740
Anbarasan K, Sharma A K, Singh R K, Deb S M, Sharma D and Mazumdar N K. 2002. Genetic relatedness among descript and non-descript goats using randomly amplified polymorphic DNA (RAPD). Indian Journal of Animal Sciences 72: 96–98.
AppaRao, K B C, Bhat K V and Totey S M. 1996. Detection of species specific genetic markers in farm animals through random amplified polymorphic DNA (RAPD). Biomolecular Engineering 13: 135–38. DOI: https://doi.org/10.1016/S1050-3862(96)00163-5
CengizElmaci, Yasemin, Oner, Seniz, Ozis and ErdoganTuncel. 2007. RAPD Analysis of DNA Polymorphism in Turkish Sheep Breeds. Biochemical Genetics 45: 691–96. DOI: https://doi.org/10.1007/s10528-007-9106-x
Chung E R, Kim W T, Kim Y S, Kim G W, Choi S H and Han S K. 1999. Breed identification of Korean native goats using DNA markers. Korean Journal of Animal Science 41: 257–70.
Ghosh S K, Choudhuri G and Gupta P. 2001. Random amplified polymorphic DNA (RAPD) assay in Indian breeds of goat. Journal of Interacademicia 5: 216–19.
Guneren G, Akyuz B and Ertugrul O. 2010. Use of RAPD-PCR for genetic analyses on the native cattle breeds in Turkey. Ankara University Veterinary FakDerg 57: 167–72. DOI: https://doi.org/10.1501/Vetfak_0000002370
Joshi M B, Rout P, Mandal A, Tyler-Smith C, Singh L and Thangaraj K. 2004.Phylogeography and origin of Indian domestic goats. Molecular Biology and Evolution 21: 454– 56. DOI: https://doi.org/10.1093/molbev/msh038
Kantanean J, Vilkki J, Elo K and Maki Tanila A. 1995. Random amplified polymorphic DNA in cattle and sheep application for detecting genetic variation. Animal Genetics 26: 315–20. DOI: https://doi.org/10.1111/j.1365-2052.1995.tb02665.x
Kemp S J and Teale A J. 1994. Randomly primed PCR amplification of pooled DNA reveals polymorphisms in a ruminant repetitive DNA sequence which differentiates Bosindicus and Bostaurus. Animal Genetics 25: 83–88. DOI: https://doi.org/10.1111/j.1365-2052.1994.tb00432.x
Lynch M. 1990. The similarity index and DNA fingerprinting. Molecular Biology and Evolution 5: 584–99.
Nei M and Li W H. 1979.Mathematical modeling for studing genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Science 76: 5296– 73. DOI: https://doi.org/10.1073/pnas.76.10.5269
Sambrook J and Russel D W. 2001. Molecular Cloning: A Laboratory Manual. Vol 1, 3rd edn. Cold Spring Harbor Laboratory Press, New York 6.4–6.12.
Sharma D, Appa Rao, K B, Singh R V and Totey S M. 2001. Genetic diversity among chicken breeds estimated through randomly amplified polymorphic DNA. Animal Biotechnology 12: 111–20. DOI: https://doi.org/10.1081/ABIO-100108337
Wei Shen, Geng-SheMin and Qin-GuoQing. 2002. Genetic analysis of the relationship between economic trait QTLs and marker genes in goat populations. Animal Biotechnology Bulletin 8: 164–70.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2016 The Indian Journal of Animal Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.