Surface expression of CD11b, CD62L, CD44 receptors on blood and milk neutrophils during subclinical and clinical mastitis in Sahiwal cows

DILIP KUMAR SWAIN; MOHAR SINGH KUSHWAH; MANDHEER KAUR; ASHOK K MOHANTY; AJAY K DANG

doi:10.56093/ijans.v86i3.56575

Authors

DILIP KUMAR SWAIN College of Veterinary Science and Animal Husbandry, Mathura
MOHAR SINGH KUSHWAH College of Veterinary Science and Animal Husbandry, Mhow
MANDHEER KAUR National Dairy Research Institute, Karnal, Haryana 132 001 India
ASHOK K MOHANTY National Dairy Research Institute, Karnal, Haryana 132 001 India
AJAY K DANG National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v86i3.56575

Keywords:

CD11b, CD44, CD62L, Mastitis, Neutrophils, Sahiwal cows

Abstract

Surface expression of cell adhering molecules and CD44 was studied in Sahiwal (SW) cows suffering naturally from subclinical (SCM) and clinical mastitis (CM). Cows were divided into groups, viz. healthy (12), SCM (12) and CM (12) groups on the basis of CMT scoring, bacteriological culture, gross morphological changes in the milk and by counting milk SCC after screening of 136 SW cows. Bacteriological culture indicated infection of Staphylococcus aureus as the causative agent of SCM and CM. Healthy cows showed significantly higher CD62L expression as compared to the SCM and CM group of animals in both blood and milk neutrophils but no significant difference was found between blood and milk neutrophils. The results revealed a significant upregulation of CD11b positive cells in the CM group of cows. Significantly higher expression of CD44 was found in the neutrophils of both blood and milk of SCM group as compared to CM group. From the study, it was evident that neutrophils exhibit dynamic interplay between the integrins (CD11b) and selectins (CD62L) along with CD44 during SCM and CM Staphylococcal mastitis to offer host protection against the foreign invaders.

Downloads

Download data is not yet available.

References

Bannerman D D. 2009. Pathogen-dependent induction of cytokines and other soluble inflammatory mediators during intra-mammary infection of dairy cows. Journal of Animal Science 87: 10–25. DOI: https://doi.org/10.2527/jas.2008-1187

Borregard N. 2010. Neutrophils from marrow to microbes. Immunity 33: 657–670. DOI: https://doi.org/10.1016/j.immuni.2010.11.011

Dang A K, Kapila S, Tomar P and Singh C. 2007. Relationship of blood and milk counts with mastitic pathogens in Murrah buffaloes. Italian Journal of Animal Science 6 (2): 821–24. DOI: https://doi.org/10.4081/ijas.2007.s2.821

Degrendele H C, Esyess P, Picker L J and Siegelman M H. 1996. CD44 and its ligand hyaluronate mediate rolling under physiologic flow: A novel lymphocyte-endothelial cell primary adhesion pathway. Journal of Experimental Medicine 183: 1119–30. DOI: https://doi.org/10.1084/jem.183.3.1119

Diez- Fraile A, Meyer E, Duchateau L and Burvenich C. 2003. L-selectin and beta2-integrin expression on circulating bovine polymorphonuclear leukocytes during endotoxin mastitis. Journal of Dairy Science 86: 2334–42. DOI: https://doi.org/10.3168/jds.S0022-0302(03)73826-0

Diez- Fraile A, Meyer E, Duchateau L and Burvenich C. 2004. Comparison of L-selectin and Mac–1 expression on blood and milk neutrophils during experimental Escherichia coli-induced mastitis in cows. American Journal of Veterinary Research 65: 1164–71. DOI: https://doi.org/10.2460/ajvr.2004.65.1164

Diez-Fraile A, Meyer E and Burvenich C. 2002. Regulation of adhesion molecules on circulating neutrophils during coliform mastitis and their possible immunomodulation with drugs. Veterinary Immunolgy and Immunopathology 86:1–10. DOI: https://doi.org/10.1016/S0165-2427(01)00432-9

Futosi K, Fodor S and Mócsai A. 2013. Neutrophil cell surface receptors and their intracellular signal transduction pathways. International Immuno Pharmacology 17: 638–50. DOI: https://doi.org/10.1016/j.intimp.2013.06.034

Hoeben D, Burvenich C, Trevisi E, Bertoni G, Hamann J, Bruckmaier R M and Blum J W. 2000. Role of endotoxin and TNF-alpha in the pathogenesis of experimentally induced coliform mastitis in periparturient cows. Journal of Dairy Research 67: 503–14. DOI: https://doi.org/10.1017/S0022029900004489

Kolaczkowska E and Cubs P.2003. Neutrophil recruitment and function in health and inflammation. Nature Review Immunology 13: 159–75. DOI: https://doi.org/10.1038/nri3399

Paape M J, Bannerman D D, Zhao X and Lee J W. 2003. The bovine neutrophil: structure and function in blood and milk. Veterinary Research 34: 597–627. DOI: https://doi.org/10.1051/vetres:2003024

Riollet C, Rainard P and Poutrel B. 2000a. Cells and cytokines in inflammatory secretions of bovine mammary gland. Advance Experimental Medical Biology 480: 247–58. DOI: https://doi.org/10.1007/0-306-46832-8_30

Riollet C, Rainard P and Poutrel B. 2000b. Differential induction of complement fragment C5a and inflammatory cytokines during intra-mammary infections with Escherichia coli and Staphylococcus aureus. Clinical Diagnostic Laboratory Immunology 7: 161–67. DOI: https://doi.org/10.1128/CDLI.7.2.161-167.2000

Sentitula, Yadav B R and Kumar R. 2012. Incidence of Staphylococci and Streptococci during winter in mastitic milk of Sahiwal cow and Murrah buffaloes. Indian Journal of Microbiology 52: 153–59. DOI: https://doi.org/10.1007/s12088-011-0207-1

Smith E M, Green L E, Medley G F, Bird H E and Dowson C G.2005. Multilocus sequence typing of Staphylococcus aureus isolated from high-somatic-cell-count cows and the environment of an organic dairy farm in the United Kingdom. Journal of Clinical Microbiology 43: 4731–36 DOI: https://doi.org/10.1128/JCM.43.9.4731-4736.2005

Swain D K, Kushwah M S, Kaur M, Patbandha T K, Mohanty A K and Dang A K. 2014. Formation of NET, phagocytic activity, surface architecture, apoptosis and expression of toll like receptors 2 and 4 (TLR2 and TLR4) in neutrophils of mastitic cows. Veterinary Research Communication 38: 209–19. DOI: https://doi.org/10.1007/s11259-014-9606-1

Swain D K, Kushwah M S, Kaur M and Dang A K. 2015a. Neutrophil dynamics in the blood and milk of crossbred cows naturally infected with Staphylococcus aureus. Veterinary World 8 (3): 336–45. DOI: https://doi.org/10.14202/vetworld.2015.336-345

Swain D K, Kushwah M S, Kaur M and Dang A K. 2015b. Elucidation of molecular basis of neutrophil apoptosis during Staphylococcal mastitis in crossbred cows. Journal of Animal Research 5 (1): 53–59. DOI: https://doi.org/10.5958/2277-940X.2015.00009.1