Porcine ovarian biometry, oocyte retrieval and quality of oocytes under different seasons in north east hill region
90 / 89
Keywords:
Follicle, Humidity, Oocytes, Ovarian biometry, PigAbstract
The present study was conducted to evaluate the effect of different seasons on porcine ovarian biometry and centred, oocyte retrieval and quality of oocytes in north east hill region of India. Porcine ovaries (1886) were collected over a period of 12 months from local abattoirs. The morphological studies revealed mulberry shaped multi- lobulated ovaries with an average weight of 4.435 ± 0.622 The average number of GF and CL were significantly higher in winter than monsoon) but not significantly different from pre-monsoon/summer. The number of CA was significantly higher in winter than monsoon and summer. The number of recovered oocyte from the visible follicles in oocyte collection medium had significantly higher number in winter than other two seasons. The oocyte percentage of grade-I, grade-II, grade-III and grade-IV were found to be 24, 36, 23 and 17%, respectively. The culturable oocytes were significantly higher during winter than summer and monsoon seasons. The season had significant effect on quality and quantity of porcine ovaries which could be well utilized in-vitro maturation and fertilization by the research in field of reproduction and biotechnology.
Downloads
References
Badinga L, Thatcher W W, Diaz T, Drost M and Wolfenson D. 1993. Effect of environmental heat stress on follicular development and steroidogenesis in lactating Holstein cows. Theriogenology 39: 797–80. DOI: https://doi.org/10.1016/0093-691X(93)90419-6
BAHS. 2010. Basic Animal Husbandry Statistics, Government of India, New Delhi.
Berger T and Roberts B M. 2009. Reduced immune-labelling of a porcine oocyte membrane protein reflects reduced fertilizability of porcine oocytes following elevated ambient temperature. Reproduction in Domestic Animals 44: 260–65. DOI: https://doi.org/10.1111/j.1439-0531.2007.01052.x
Bertoldo M, Grupen C G, Thomson P C, Evans G and Holyoake P K. 2009. Identification of sow-specific risk factors for late pregnancy loss during the seasonal infertility period in pigs. Theriogenology 72: 393–400. DOI: https://doi.org/10.1016/j.theriogenology.2009.03.008
Bertoldo M, Holyoake P K, Evans G and Grupen C G. 2010. Oocyte developmental competence is reduced in sows during the seasonal infertility period. Reproductive Fertility and Development 22:1222–29. DOI: https://doi.org/10.1071/RD10093
Bertoldo M, Holyoake P K, Evans G and Grupen C G. 2011. Follicular progesterone levels decrease during the period of seasonal infertility in sows. Reproduction Domestic Animal 46: 489–94. DOI: https://doi.org/10.1111/j.1439-0531.2010.01695.x
Claus R and Weiler U.1985. Influence of light and photoperiodicity on pig prolificacy. Journal of Reproduction and Fertility, Supplement 33: 185–97.
Davis M S, Mader T L, Holt S M and Parkhurst A M. 2003. Strategies to reduce feedlot cattle heat stress: Effects on tympanic temperature. Journal of Animal Science 81: 649– 61. DOI: https://doi.org/10.2527/2003.813649x
Finocchiaro R, Van Kaam J, Portolano B and Misztal I. 2005. Effect of heat stress on production of Mediterranean dairy sheep. Journal of Dairy Science 88:1855–64. http://dx.doi.org/ 10.3168/jds.S0022–0302(05)72860–5. DOI: https://doi.org/10.3168/jds.S0022-0302(05)72860-5
Gaughan J B, Holt S M, Hahn G L, Mader T L and Eigenberg R. 2000. Respiration rate – is it a good measure of heat stress in cattle? Asian Australasian Journal of Animal Science 13:329– 32 (ARD No. 12903).
Kelly C F and Bond T E. 1971. Bioclimatic Factors and Their Measurement: A Guide to Environmental Research on Animals. National Academy Science, Washington, DC.
Love R J. 1978. Definition of a seasonal infertility problem in pigs. Veterinary Record 103: 443–46. DOI: https://doi.org/10.1136/vr.103.20.443
Mader T L. 2003. Environmental stress in confined beef cattle. Journal of Animal Science 81 (electronic suppl. 2):110– 119 (ARD No. 13820).
Mihm M, Curran N, Hyttel P, Knight P G, Boland M P and Roche J F. 1999. Effect of dominant follicle persistence on follicular fluid oestradiol and inhibin and on oocyte maturation in heifers. Journal of Reproductive Fertility 116:293–304. DOI: https://doi.org/10.1530/jrf.0.1160293
Paterson A M, Pearce G P and Antuono M F. 1991. Seasonal variation in attainment of puberty in isolated and boar-exposed domestic gilts. Animal Reproduction Science 24: 323–33. DOI: https://doi.org/10.1016/S0378-4320(05)80015-6
Prunier A, Dourmad J Y and Etienne M. 1994. Effect of light regimen under various ambient temperatures on sow and litter performance. Journal of Animal Science 72:1461–66. DOI: https://doi.org/10.2527/1994.7261461x
Roth Z, Meidan R, Braw-Tal R and Wolfenson D. 2000. Immediate and delayed effects of heat stress on follicular development and its association with plasma FSH and inhibin concentration in cows. Journal Reproductive Fertility 120: 83–90. DOI: https://doi.org/10.1530/jrf.0.1200083
Snedecor G W and Cochran W G. 1994. Statistical methods. 8th edn. Oxford and IBH Publishing Co., Kolkatta.
Tast A, Peltoniemi O A, Virolainen J V and Love R J. 2002. Early disruption of pregnancy as a manifestation of seasonal infertility in pigs: an article describing fertility problems associated to seasonal reproductive patterns in pigs. Animal Reproduction Science 74: 75–86. DOI: https://doi.org/10.1016/S0378-4320(02)00167-7
WilsonS J, Marion R S, Spain J N, Spiers D E, Keisler D H and Lucy M C. 1998. Effects of controlled heat stress on ovarian function of dairy cattle. 1. Lactating cows. Journal of Dairy Science 81: 2124–31. DOI: https://doi.org/10.3168/jds.S0022-0302(98)75788-1
Wolfenson D, Thatcher WW, Badinga L, Savio J D, Meidan R, Lew B J, Braw-Tal R and Berman A. 1995. Effect of heat stress on follicular development during the oestrous cycle in lactating dairy cattle. Biology of Reproduction 52: 1106–13. DOI: https://doi.org/10.1095/biolreprod52.5.1106
Wolfenson D, Lew B J, Thatcher W W, Graber and Meidan R. 1997. Seasonal and acute heat stress effects on steroid production by dominant follicles in cows. Animal Reproduction Science 47: 9–19. DOI: https://doi.org/10.1016/S0378-4320(96)01638-7
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2016 The Indian Journal of Animal Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.