Effect of urea supplementation in diet based on barley grain or corn silage on performance, digestion, rumen fermentation and microbial protein synthesis in Holstein bull calves

M KAZEMI-BONCHENARI; A Z M SALEM; E GHASEMI

doi:10.56093/ijans.v86i3.56709

Authors

M KAZEMI-BONCHENARI Arak University, Arak 38156-8-8349 Iran
A Z M SALEM Universidad Autónoma del Estado de México, México
E GHASEMI Isfahan University of Technology, 84156- 83111, Isfahan, Iran

https://doi.org/10.56093/ijans.v86i3.56709

Keywords:

Bull calves, Carbohydrate sources, Microbial nitrogen, Rumen fermentation

Abstract

This study was aimed to evaluate the effect of non-protein nitrogen source of urea with barley grain (BG) or corn silage (CS) based diet in Holstein bull calves. Nutrient digestibility, rumen fermentation activities, microbial nitrogen yield (MN) and blood metabolites were determined. Holstein bull calves (14), weighing 126 ± 4.2 kg were allocated in 2 different diets based on BG or CS for 9 weeks. Diets were based on rolled barley grain (BG) and on finely chopped corn silage (CS). Both diets were supplemented with 7.5 g/kg urea on DM basis. Intake and performance of animal did not differ between diets, whereas digestibility of neutral detergent fiber (NDF) decreased in BG diet. Ruminal concentration of propionate and blood glucose concentration increased in BG calves, whereas ruminal pH and acetate concentration increased in CS fed calves. The ruminal NH₃-N was increased in CS diet, while excretions of allantoin, purine derivatives and estimated MN yield through urine were greater in BG diet vs. CS diet. In conclusion, the results suggested that increased urinary PD excretion and consequently increased estimated MN yield value for BG diet presented more potential for this diet to be synchronized with urea in calves’ rumen digestion.

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References

AOAC. 1995. Official Methods of Analysis. 17th edn. Association of Official Analytical Chemists, Arlington, VA.

Beauchemin K A, Yang W Z and Rode L M. 2001. Effects of barley grain processing on the site and extent of digestion of beef feedlot finishing diets. Journal of Animal Science 79: 1925–36. DOI: https://doi.org/10.2527/2001.7971925x

Bourquin L D, Titgemeyer E C, Van Milgen J and Fahey G C. 1994. Forage level and particle size effects on orchardgrass digestion by steers: II. Ruminal digestion kinetics of cell wall components. Journal of Animal Science 72: 759–67. DOI: https://doi.org/10.2527/1994.723759x

Brito A F and Broderick G A. 2007. Effects of different protein supplements on milk production and nutrient utilization in lactating dairy cows. Journal of Dairy Science 90: 1816–27. DOI: https://doi.org/10.3168/jds.2006-558

Broderick G and Clayton M K. 1997. A statistical evaluation of animal and nutritional factors influencing concentrations of milk urea nitrogen. Journal of Dairy Science 80: 2964–71. DOI: https://doi.org/10.3168/jds.S0022-0302(97)76262-3

Broderick G A. 2003. Effects of varying dietary protein and energy levels on the production of lactating dairy cows. Journal of Dairy Science 86: 1370–81. DOI: https://doi.org/10.3168/jds.S0022-0302(03)73721-7

Chen X B and Gomes M J. 1992. Estimation of microbial protein supply to sheep and cattle based on urinary excretion of purine derivatives: An overview of technical details. International Feed Research Unit, Occasional Publications. Rowett Research Institute, Aberdeen, United Kingdom.

Chizzotti M L, Valadares Filho S C, Valadares R F D, Chizzotti F H M and Tedeschi L O. 2008. Determination of creatinine excretion and evaluation of spot urine sampling in Holstein cattle. Livestock Science 113: 218–25. DOI: https://doi.org/10.1016/j.livsci.2007.03.013

Crooke W M and Simpson W E. 1971. Determination of ammonia in kjeldahl digests of crops by an automated procedure. Journal of the Science of Food and Agriculture 22: 903–16. DOI: https://doi.org/10.1002/jsfa.2740220104

Firkins J L. 1997. Effects of feeding non-forage fiber sources on site of fiber digestion. Journal of Dairy Science 80: 1426–37. DOI: https://doi.org/10.3168/jds.S0022-0302(97)76072-7

Gandra J R, Freitas Jr J E, Barletta A R V, Maturana Filho M, Gimenes L U, Vilela F G, Baruselli P S and Renno F P. 2011. Productive performance, nutrient digestion and metabolism of Holstein (Bos taurus) and Nellore (Bos taurus indicus) cattle and Mediterranean buffaloes (Bubalis bubalis) fed with corn silage based diets. Livestock Science 140: 283–91. DOI: https://doi.org/10.1016/j.livsci.2011.04.005

Ghasemi, E Khorvash M, Ghorbani G R, Hashemzadeh F, Saebi- Far M, Kahyani Aand Kazemi-Bonchenari M. 2014. Interaction effects of degradable nitrogen sources and straw treatment on rumen parameters and microbial protein synthesis in sheep. The Indian Journal of Animal Sciences 84 (9): 1011–15.

Jones D F, Hoover W H and Webster T K M. 1998. Effect of concentration of peptides on microbial metabolism in continuous culture. Journal of Animal Science 76: 611–16. DOI: https://doi.org/10.2527/1998.762611x

Kohn R A, Dinnen M M and Russek-Cohen E. 2005. Using blood urea nitrogen to predict nitrogen excretion and efficiency of nitrogen utilization in cattle, sheep, goats, horses, pigs and rats. Journal of Animal Science 83: 879–89. DOI: https://doi.org/10.2527/2005.834879x

Plascencia A and Zinn R A. 1996. Influence of flake density on the feeding value of steam-processed corn in diets for lactating cows. Journal of Animal Science 74: 310–16. DOI: https://doi.org/10.2527/1996.742310x

Popova M, Martin C, Eugene M, Mailon M M, Doreau M and Morgavi D P. 2011. Effect of fiber- and starch-rich finishing diets on methanogenic Archaea diversity and activity in the rumen of feedlot bulls. Animal Feed Science and Technology 166–167: 113–21. DOI: https://doi.org/10.1016/j.anifeedsci.2011.04.060

Rodriquez-Prado M, Calsamiglia S and Ferret A. 2004. Effects of fiber content and particle size of forage on the flow of microbial amino acids from continuous culture fermenters. Journal of Dairy Science 87: 1413–24. DOI: https://doi.org/10.3168/jds.S0022-0302(04)73290-7

Russell J B, O’conor J D, Fox D G, Van Soest P J and Sniffen C J. 1992. A net carbohydrate and protein system for evaluating cattle diets: I. Ruminal fermentation. Journal of Animal Science 70: 3551–61. DOI: https://doi.org/10.2527/1992.70113551x

SAS,1999–2000. SAS/STAT User’s Guide, Version 8. SAS Institute Inc., Cary, NC.

Valadares R F D, Broderick G A, Valadares Filho S C and Clayton M K. 1999. Effect of replacing alfalfa silage with high moisture corn on ruminal protein synthesis estimated from excretion of total purine derivatives. Journal of Dairy Science 82: 2686– 96. DOI: https://doi.org/10.3168/jds.S0022-0302(99)75525-6

Van Kulen J and Young B A. 1977. Acid insoluble ash as a natural marker for digestibility studies. Journal of Animal Science 44: 282–87. DOI: https://doi.org/10.2527/jas1977.442282x

Van Soest P J, Roberts J B and Lewis B A. 1991. Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74: 3583–97. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2