PCR based detection of toxoplasmosis in tissue samples: A step towards detection of toxoplasmosis in meat and post mortem samples
![](/public/icon/gor.png)
![](/public/icon/pdf.png)
Keywords:
PCR based detection, SAG 3 amplicon, Tissue samples, ToxoplasmosisAbstract
The aim of the study was to find out a suitable marker gene for diagnosis of toxoplasmosis in tissue and meat samples during meat inspection and/or during post mortem studies. PCR was used to diagnose toxoplasmosis in ten inbred Swiss albino mice after experimental inoculation of 100 tachyzoites of laboratory maintained human RH strain of the parasite. Blood, peritoneal lavage and tissue from lung, liver spleen, brain, heart and kidney were taken from experimental murine models in duplicate. The samples were subjected to PCR, using primers directed to the multicopy of Surface Antigen 3 (SAG 3 gene). Blood, kidney and heart tissue were found negative while peritoneal lavage along with lung, liver spleen and brain tissue yielded desired positive amplicons. The significance of the studied molecule vis-Ã -vis future projections in regard to diagnosis of toxoplasmosis during meat sample examination and/or during routine post mortem is being described.Downloads
References
Burg J L, Grover C M, Pouletty P, Boothroyd J C. 1989. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. Journal of Clinical Microbiology 27: 1787–92. DOI: https://doi.org/10.1128/jcm.27.8.1787-1792.1989
Dubey J P and Beattie C P. 1988. Toxoplasmosis of Animals and Man. CRC Press, Boca Raton, Florida.
Tenter A M, Heckeroth A R and Weiss L M. 2000. Toxoplasma gondii: From animal to humans. International Journal of Parasitology 30: 1217–58. DOI: https://doi.org/10.1016/S0020-7519(00)00124-7
Hurtado A, Aduriz G, Moreno B, Barandika J, Garcia-Pe´rez A L. 2001. Single tube nested PCR for the detection of Toxoplasma gondii in fetal tissues from naturally aborted ewes. Veterinary Parasitology 102: 17–27. DOI: https://doi.org/10.1016/S0304-4017(01)00526-X
Jacquet A, Coulon L, Neve J D, Daminet V, Haumont M and Garcia L. 2001. The surface antigen SAG3 mediates the attachment of Toxoplasma gondii to cell-surface proteoglycans. Molecular and Biochemical Parasitology 116: 35–44. DOI: https://doi.org/10.1016/S0166-6851(01)00297-3
MacPherson J M and Gajadhar A A. 1993. Sensitive and specific polymerase chain reaction detection of Toxoplasma gondii for veterinary and medical diagnosis. Canadian Journal of Veterinary Research 57: 45–48.
Sreekumar C, Rao J R, Mishra A K, Ray D, Joshi P and Singh R K. 2004. Detection of toxoplasmosis in experimentally infected goats by PCR. Veterinary Record 15: 632–35. DOI: https://doi.org/10.1136/vr.154.20.632
Sudan V, Tewari A K, Singh H, Saravanan B C and Sankar M. 2012. Molecular characterizationof surface antigen 3 (SAG3) gene of Toxoplasma gondii RH-IVRI strain. Journal of Parasitic Diseases 36 (2): 210–14. DOI: https://doi.org/10.1007/s12639-012-0107-2
Sudan V, Tewari A K and Singh H. 2014a. An insight into the behavior, course and kinetics of acute infection of Toxoplasma gondii human RH strain in experimentally infected murine model. Iranian Journal of Parasitology 9 (1): 114–19.
Sudan V, Tewari A K, Singh H and Singh R. 2014b. Pathobiology of human RH strain induced experimental toxoplasmosis in murine model. Journal of Parasitic Diseases DOI 10.1007/s12639-014-0589–1. DOI: https://doi.org/10.1007/s12639-014-0589-1
Wastling J M, Nicoll S and Buxton D. 1993. Comparison of two gene amplification methods for the detection of Toxoplasma gondii in experimentally infected sheep. Journal of Medical Microbiology 38: 360–65. DOI: https://doi.org/10.1099/00222615-38-5-360
Yai L E O, Vianna M C B, Soares R M, Cortez A, Freire R L and Richtzenhain L J. 2003. Evaluation of experimental Toxoplasma gondii (Nicolle and Manceaux 1909) infection in pigs by bioassay in mice and polymerase chain reaction. Brazilian Journal of Veterinary Research and Animal Sciences 40: 227–34. DOI: https://doi.org/10.1590/S1413-95962003000300010
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2016 The Indian Journal of Animal Sciences
![Creative Commons License](http://i.creativecommons.org/l/by-nc-sa/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.