Genetic polymorphism of caprine stearoyl-coA desaturase (SCD) gene and their relationship with blood cholesterol, triglyceride and pre-weaning growth traits of mixed breeds of goats in southern Thailand

CHINA SUPAKORN; WINAI PRALOMKARN; SORNTHEP TUMWASORN

doi:10.56093/ijans.v86i4.57776

Authors

CHINA SUPAKORN School of Agricultural Technology,Walailak University, Nakhon Si Thammarat 80161 Thailand
WINAI PRALOMKARN School of Agricultural Technology,Walailak University, Nakhon Si Thammarat 80161 Thailand
SORNTHEP TUMWASORN School of Agricultural Technology,Walailak University, Nakhon Si Thammarat 80161 Thailand

https://doi.org/10.56093/ijans.v86i4.57776

Keywords:

Blood cholesterol, Goat, Pre-weaning growth traits, Stearoyl-coA desaturase (SCD) gene, Triglyceride

Abstract

The present study attempts to identify genetic polymorphisms of stearoyl-coA desaturase (SCD) gene and their relationship with blood cholesterol, triglyceride and pre-weaning growth traits in a goat population in southern Thailand. Genetic variability in caprine SCD was analyzed in 290 animals belonging to several types of Thai native (TN), Anglo-nubian (AN), Boer (B) and Saanen (SA) breed crosses by PCR-SSCP and DNA sequencing. Four SNPs were identified in exon3 (A/G), exon5 (C/T), exon6 (C/G) and 3’untranslated region (3’UTR) (TGT deletion). Five haplotypes (A, B, C, D and E) were constructed. Haplotype frequency B was the highest (0.50) but haplotype frequency C was the lowest (0.01). Haplotype effect of SCD gene had influenced on blood cholesterol, triglyceride, weaning weight and growth rate. Individuals with haplotype C had significant lowest triglyceride, weaning weight and growth rate (P<0.05). Also, this haplotype could be culled for growth improvement in this population.

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References

Chen Z, Sun J, Li Z, Lan X, Zhang C, Qu Y, Liu Y, Fang X, Lei C and Chen H. 2011. Novel SNPs in caprinestearoyl-CoA desaturase (SCD) and decorin (DCN) genes that are associated with growth traits in Chinese breeds. Molecular Biology Report 38 (5): 3121–27. DOI: https://doi.org/10.1007/s11033-010-9982-8

Dong Y, Xie M, Jiang Y, Xiao N, Du X, Zhang W, Tosser-Klopp G, Wang J, Yang S, Liang J, Chen W, Chen J, Zeng P, Hou Y, Bian C, Pan S, Li Y, Liu X, Servin B, Sayre B, Zhu B, Sweeney D, Moore R, Nie W, Shen Y, Zhao R, Zhang G, Li J, Faraut T, Womack J, Zhang Y, Kijas J, Cockett N, Xu X, Zhao S, Wang J and Wang W. 2013. Sequencing and automated whole- genome optical mapping of the genome of a domestic goat (Capra hircus). Nature Biotechnology 31 (2): 135–41. DOI: https://doi.org/10.1038/nbt.2478

Garcia-Fernandez M, Gutierrez-Gill B, Garcia-Gamez E and Arranz J J. 2009. Genetic variability of the stearoyl-CoA desaturase gene in sheep. Molecular and Cellular Probes 23 (2): 107–11. DOI: https://doi.org/10.1016/j.mcp.2009.01.001

R PROGRAM. 2010. R program version 2.11.1. Institute for Statistics and Mathematics, GNU General Public License, Boston, USA.

Samardzija M, Vince S and Duricic D. 2013. Association of parity, fecundity and body condition score with blood serum concentration of some metabolites during pre and post parturient period in German Improved Fawn goats. Veterinarski Arhiv 83 (5): 469–77.

Supakorn C and Pralomkarn W. 2009. Pre-weaning growth of goats for meat raised on a commercial farm in southern Thailand. Thai Journal of Agricultural Science 42 (1): 13–19.

Zhang C L, Gao X Y, Shao R Y, Wang Y H, Fang X T and Chen H. 2010. Stearoyl-CoA desaturase (SCD) gene polymorphism in goat breeds. Biochemical Genetics 48 (9–10): 822–28. DOI: https://doi.org/10.1007/s10528-010-9363-y

Zidi A, Fernandes-Cabanas V M, Urrutia B, Carrizosa J, Polvillo O, Gonzalez-Redondor P, Jordana J, Gallardo D, Amills M and Serradilla J M. 2010. Association between the polymorphism of the goat stearoyl-CoA desaturase1 (SCD1) gene and milk fatty acid composition in Murciano-Granadina goats. Journal of Dairy Science 93 (3): 4332–39. DOI: https://doi.org/10.3168/jds.2009-2597