Evaluation of mannan-oligosaccharides (MOS) in broiler chicken during hot humid summer using zoo technical, molecular and physio-biochemical tools

J J ROKADE; S K BHANJA; A S SHINDE; SAJAD; MUKESH KAPGATE; A B MANDAL

doi:10.56093/ijans.v86i4.57790

Authors

J J ROKADE ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
S K BHANJA ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A S SHINDE ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
SAJAD ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
MUKESH KAPGATE ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A B MANDAL ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v86i4.57790

Keywords:

Broiler, Corticosterone, Hot-humid conditions, HSP-70, Immunity, MOS

Abstract

Climate resilient poultry production is a major challenge especially for hot regions like India. Accordingly, the efficacy of mannan-oligosaccharides as anti-heat stressor was studied. Broiler chicks were reared on a standard diet up to 14^th day of age. Thereafter, the chicks were randomly distributed into three dietary treatment groups viz. T1 (Control group: Standard diet, T2 (Standard diet with MOS @ 0.3%) and T3 (Standard diet with MOS @ 0.5%) each with 40 birds divided in 5 replicates of 8 birds each upto 42 days of age. Experiment was carried out during hot-humid (August-September, 26.0±0.12° to 34.25±0.37°C, Rh%: 76.95±0.90 to 86.15±0.61) summer. Growth performance, immunity (4^th week), physiological (4^th and 6^th week), biochemical (4^th and 6^th wk) and molecular parameters (4^th and 6^th wk) were recorded. Feed intake, live weight gain and FCR improved significantly (P<0.001) on MOS supplementation. The relative yield of immune organs at 4^th and 6^th week of age, humoral (P<0.001) as well as cellular (P<0.01) immunity also improved significantly. The percentage of haemoglobin, protein, aspartate transaminase and alanine transaminase increased significantly (P<0.001) due to MOS supplementation at 4^th as well as 6^th week of age. While H:L ratio, serum corticosterone and serum cholesterol decreased significantly (P<0.001) in MOS supplemented groups. Supplementation of MOS at both the levels (0.3 and 0.5%) caused significant down regulation of relative expression of HSP70 in jejunum tissues during 28^th or 42^nd day of age. From the results of the present study, it can be concluded that MOS supplementation @ 0.3% or 0.5% in diet of heat stressed broilers improved performance as well as welfare.

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