Sperm mitochondrial membrane potential and motility pattern in the Holstein bull semen positive for heparin binding proteins

G KRISHNAN; A THANGVEL; K LOGANATHASAMY; C VEERAPANDIAN; P KUMARASAMY; M KARUNAKARAN

doi:10.56093/ijans.v86i5.58455

Authors

G KRISHNAN National Research Centre on Yak, Dirang
A THANGVEL Madras Veterinary College, Chennai, Tamil Nadu 600 007 India
K LOGANATHASAMY Madras Veterinary College, Chennai, Tamil Nadu 600 007 India
C VEERAPANDIAN Veterinary College and Research Institute, Orathnadu
P KUMARASAMY Madras Veterinary College, Chennai, Tamil Nadu 600 007 India
M KARUNAKARAN National Dairy Research Institute, ERS, Kalyani, West Bengal

https://doi.org/10.56093/ijans.v86i5.58455

Keywords:

Heparin binding proteins, Mitochondrial membrane potential, Motility, Plasma membrane integrity, Velocity

Abstract

Present study was aimed to evaluate sperm motility and mitochondrial membrane potential (MMP) in relation to the presence of heparin binding proteins (HBPs) in semen. Semen was collected from 17 Holstein Friesian breeding bulls. Seminal plasma and sperm membrane proteins were isolated by precipitation method; HBPs were isolated using heparin-sepharose affinity chromatography. SDS-PAGE was performed and bulls were categorized into 3 groups based on presence/absence of 24 and 30 kDa HBPs. Computer-assisted semen analysis revealed that fresh ejaculates of bulls positive for 30 kDa HBP had 4.24 and 14.18% of higher total motile sperm than semen with 24 kDa and negative for HBPs. Semen with 30 and 24 kDa had 18.72 and 12.34% of higher number of sperm with progressive motility and velocity than semen devoid of HBPs. Simultaneous assessment of plasma membrane integrity (PMI) and MMP by fluorescence staining revealed that post-thaw sperm of semen with 30 kDa HBP had 6.28 and 12.79% of higher intact PMI. Further, sperm of semen with 30 kDa HBP had 4.61 and 10.50% of higher MMP, which had travelled an extra distance of 9.71 and 4.95 mm through cervical mucus than semen with 24 kDa and devoid of HBPs. The mucus penetration distance showed a strong positive correlation with high MMP, PMI, sperm motility and velocity. It is to be concluded that HBPs were able to sustain higher percentage of motile sperm with progressive motility and MMP which was depicted by more mucus penetration distance.

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References

Aitken R J and Baker M A. 2013. Causes and consequences of apoptosis in spermatozoa: contributions to infertility and impacts on development. International Journal of Developmental Biology 57: 265–72. DOI: https://doi.org/10.1387/ijdb.130146ja

Ajaoa C, Anderssona M A, Teplova V V, Nagyc S, Gahmberg C G, Anderssone L C, Hautaniemif M, Kakasi B, Roivainenh M and Salkinoja-Salonena M. 2015. Mitochondrial toxicity of triclosan on mammalian cells. Toxicology Reports 2: 624–37. DOI: https://doi.org/10.1016/j.toxrep.2015.03.012

Ardon F and Suarez S S. 2013. Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins DOI: https://doi.org/10.1530/REP-12-0468

BSP1, BSP3 and BSP5. Reproduction 146: 111–17.

Asadpour R, Alavi-Shoushtari S M, Asri Rezaii S, Khan M H and Ansari K M. 2007. SDS-polyacrylamide gel electrophoresis of buffalo bulls seminal plasma proteins and their relation with semen freezability. Animal Reproduction Science 102: 308–13. DOI: https://doi.org/10.1016/j.anireprosci.2007.03.003

Ashrafi I, Kohram H and Tayefi-Nasrabadi H. 2013. Antioxidant effects of bovine serum albumin on kinetics, microscopic and oxidative characters of cryopreserved bull spermatozoa. Spanish Journal of Agricultural Research 11 (3): 695–701. DOI: https://doi.org/10.5424/sjar/2013113-3870

Chaveiro A, Cerqueira C, Silva J, Franco J and Moreira da Silva F. 2015. Evaluation of frozen thawed cauda epididymal sperms and in vitro fertilizing potential of bovine sperm collected from the cauda epididymal. Iranian Journal of Veterinary Research 16 (2): 188–93.

Espinoza J A, Paasch U and Villegas J V. 2009. Mitochondrial membrane potential disruption pattern in human sperm. Human Reproduction 24 (9): 2079–85. DOI: https://doi.org/10.1093/humrep/dep120

Gohar A, Khan H, Yousaf M S, Ahmad I, Ali Q, Khan M, Khan D, Hayat Y, Ali F, Salim M and Ullah F. 2014. Assessment of alpha lipoic acid inclusion in semen extender on cryopreservation of Nili-Ravi buffalo bull spermatozoa. Life Science Journal 11 (9): 45–50.

Goularte K L, Gastal G D A, Schiavon R S, Goncalves A O, Schneider J R, Corcini C D and Lucia T. 2014. Association between the presence of protein bands in ram seminal plasma and sperm tolerance to freezing. Animal Reproduction Science 146: 165–69. DOI: https://doi.org/10.1016/j.anireprosci.2014.03.009

Grunewald S, Said T M, Paasch U, Glander H J and Agarwal A. 2008. Relationship between sperm apoptosis signalling and oocyte penetration capacity. International Journal of Andrology 31: 325–30. DOI: https://doi.org/10.1111/j.1365-2605.2007.00768.x

Jobim M I M, Oberst E R, Salbego C G, Souza D O, Wald V B, Tramontina F and Mattos R C. 2004. Two-dimensional polyacrylamide gel electrophoresis of bovine seminal plasma proteins and their relation with semen freezability. Theriogenology 61: 255–66. DOI: https://doi.org/10.1016/S0093-691X(03)00230-9

Karunakaran M, Devanathan TG, Kulasekar K, Sridevi P, Jawahar TP, Loganathsamy K, Dhali A, Selvaraju S. 2012. Effect of heparin binding protein and hydrogen peroxide on lipid peroxidation status of bovine sperm cells. Indian Journal of Animal Sciences 82 (9): 976–78.

Karunakaran M, Devanathan T G, Jawahar T P, MANIMARAN K, Chitra A, Dhali A and selvaraju S. 2013. Effect of heparin binding proteins on the in vitro sperm characters and lipid peroxidation status of frozen thawed bull semen. Indian Journal of Animal Sciences 83 (8): 788–90.

Kim E Y, Noh E H, Noh E J, Park M J, Park H Y, Lee D S, Riu K Z and Park S P. 2013. Effect of glycosaminoglycans on in vitro fertilizing ability and in vitro developmental potential of bovine embryos. Asian-Australian Journal of Animal Science 26 (2): 178–88. DOI: https://doi.org/10.5713/ajas.2012.12406

Kumar P, Monika Saini M, Kumar D, Balhara A K, Yadav S P, Singh P and Yadav P S. 2015. Liposome-based semen extender is suitable alternative toegg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Animal Reproduction Science 159: 38–45. DOI: https://doi.org/10.1016/j.anireprosci.2015.05.010

Kutty M V H, Remya V, Shyma V H, Radhika S, Shende A M and Bhure S K. 2014. An overview on binder of sperm proteins of cattle. International Journal of Livestock Research 4 (4): 1–11. DOI: https://doi.org/10.5455/ijlr.20140629034437

Laemmli V K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 660– 85. DOI: https://doi.org/10.1038/227680a0

Malik A, Laily M and Zakir M I. 2015. Effects of long term storage of semen in liquid nitrogen on the viability, motility and abnormality of frozen thawed Frisian Holstein bull spermatozoa. Asian Pacific Journal of Reproduction 4 (1): 22– DOI: https://doi.org/10.1016/S2305-0500(14)60052-X

Manaskova P, Liberda J V, Ticha M and Jonakova V. 2002. Isolation of non-heparin-binding and heparin-binding proteins of boar prostate. Journal of Chromatology 770: 137–43. DOI: https://doi.org/10.1016/S0378-4347(01)00480-7

Manjunath P, Lefebvre J, Jois P S, Fan J and Wright M W. 2009. New nomenclature for mammalian BSP genes. Biology of Reproduction 80: 394–97. DOI: https://doi.org/10.1095/biolreprod.108.074088

Mogielnicka-Brzozowska M, Dziekonska A, Strzezek R, Zalecki M, Majewska A, Tolscik K and Kordan W. 2014. Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C. Bulletin of the Veterinary Institute in Pulawy 58: 163– 68. DOI: https://doi.org/10.2478/bvip-2014-0025

Moura A A, Chapman D A and Killian G J. 2007. Proteins of the accessory sex glands associated with the oocyte-penetrating capacity of cauda epididymal sperm from Holstein bulls of documented fertility. Molecular Reproduction Development 74: 214–22. DOI: https://doi.org/10.1002/mrd.20590

Murase T and Braun J W. 1990. Impact of methodological factors on sperm penetration into cervical mucus in cattle. Theriogenology 34: 73–80. DOI: https://doi.org/10.1016/0093-691X(90)90578-H

Najjar A, Chetoui C and Ben Mrad M. 2013. Evaluation of post thawed semen motility of Holstein bulls by computer assisted sperm analysis (CASA). The Experiment 14 (2): 894–96.

Naseer Z, Ahmad E and Aksoy M. 2014. Cholesterol efflux from sperm: approaches and applications. Turkish Journal of Veterinary and Animal Sciences 38: 1404–55. DOI: https://doi.org/10.3906/vet-1404-55

Papa P M, Maziero R D, Guasti P N, Junqueira C R, Freitas-Dell Aqua C P, Papa F O, Vianna F P, Alvarenga M A, Crespilho A M and Dell Aqua J A. 2015. Effect of glycerol on the viability and fertility of cooled bovine semen. Theriogenology 83: 107– 13. DOI: https://doi.org/10.1016/j.theriogenology.2014.08.009

Parisi A, Thompson M S K, Kaya A and Memili E. 2014. Molecular, cellular and physiological determinants of bull fertility. Turkish Journal of Veterinary and Animal Sciences 38: 1–6. DOI: https://doi.org/10.3906/vet-1404-76

Ramteke S S, Shende A M, Rajoriya J S, Barik N C, Pande M, Bhure S K, Das G K, Ghosh S K, Prasad J K. 2014. Comparative study of heparin-binding proteins profile of Murrah buffalo (Bubalus bubalis) semen. Veterinary World 7 (9): 707–11. DOI: https://doi.org/10.14202/vetworld.2014.707-711

Reis L S L S, Ramos A A, Camargos A S and Oba E. 2014. Effect of manganese supplementation on the membraneintegrity and the mitochondrial potential of the sperm of grazing Nelore bulls. Animal Reproduction Science 150 (1–2): 1–6. DOI: https://doi.org/10.1016/j.anireprosci.2014.06.033

Sarsaifi K, Vejayan J, Haron A W, Yusoff R, Hani H, Rasoli M, Omar M A and Othman A M. 2015. Protein profile and functionality of spermatozoa from two semen collection methods in Bali bulls. Livestock Science 172: 96–105. DOI: https://doi.org/10.1016/j.livsci.2014.12.004

Selvaraju S, Ghosh J and Ravindra J P. 2009. Prognostic value of various spermatological attributes as predictors of zona binding and zona penetration of buffalo (Bubalus bubalis) semen. Reproduction in Domestic Animals 44: 6–11. DOI: https://doi.org/10.1111/j.1439-0531.2007.00979.x

Singh A K, Brar P S and Cheema R S. 2014. Relationships among frozen-thawed semen fertility, physical parameters, certain routine sperm characteristics and testosterone in breeding Murrah buffalo (Bubalus bubalis) bulls. Veterinary World 7 (9): 644–651. DOI: https://doi.org/10.14202/vetworld.2014.644-651

Souza F, Martins M I M, Fernandes C E S, Ribolla P E M and Lopes M D. 2006. Heparin-binding proteins of canine seminal plasma. Theriogenology 66: 1606–09. DOI: https://doi.org/10.1016/j.theriogenology.2006.02.016

Srivastava S and Kumar S. 2014. Incorporation of ascorbic acid, caffeine and chloroquine diphosphate in dilutor improves structural and functional status of frozen semen. Open Access Library Journal 1: e011. http://dx.doi.org10.4236/oalib.1100011. DOI: https://doi.org/10.4236/oalib.1100011

Tas M, Bacinoglu S, Cirit U, Ozdas O B and Ak K. 2007. Relationship between bovine fertility and the number of spermatozoa penetrating the cervical mucus within straws. Animal Reproduction Science 101: 18–27. DOI: https://doi.org/10.1016/j.anireprosci.2006.08.020

Yoon S J, Kwon W S, Rahman M S, Lee J S, Pang M G. 2015. A novel approach to identifying physical markers of cryo-damage in bull spermatozoa. PLoS ONE 10 (5): e0126232. doi: 10.1371/journal.pone.0126232. DOI: https://doi.org/10.1371/journal.pone.0126232

Zodinsanga V, Mavi P S, Cheema Ranjna S, Kumar A, Gandotra V K. 2015. Relationship between routine analysis/sperm function and fertility tests of cattle bull semen. Asian Journal of Animal Sciences 9 (1): 18–25. DOI: https://doi.org/10.3923/ajas.2015.37.44