Effect of glutathione on the quality of frozen buck semen
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https://doi.org/10.56093/ijans.v86i5.58473
Keywords:
Beetal buck, Freezing, Glutathione, Semen qualityAbstract
Ejaculates (30) collected twice weekly from five Beetal bucks were used to study the effect of addition of 0 (control), 4 mM, 6 mM and 8 mM glutathione on the quality of frozen Beetal buck semen by split sample technique. After removal of seminal plasma, the semen was primarily extended with Tris extender (1: 5) considering the volume of semen prior to removal of seminal plasma and then split into 4 parts and finally extended with equal volume of Tris extender that rose the extension rate to 1: 10. The mean percentage of sperm motility, live sperm, live intact acrosome, HOST-reacted sperm was significantly higher in Tris extender containing 4 mM glutathione than that containing 6 mM, 8 mM glutathione and control. The release of ALT and AST from post-thaw spermatozoa was also the lowest in semen containing 4mM concentration of glutathione. Based on post-thaw sperm motility, live sperm, live intact acrosome, HOST-reacted sperm, and ALT and AST release, addition of 4 mM glutathione in Tris extender was superior to 6 mM, 8 mM and 0 mM (control). It was concluded that glutathione at 4 mM could be used as an antioxidant in Tris for cryopreservation of Beetal buck semen which could provide a better environment in protecting the functional capacity of spermatozoa.
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Bilodeau J F, Chatterjee S, Sirard M A and Gagnon C. 2000. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Molecular Reproduction and Development 55: 282–88. DOI: https://doi.org/10.1002/(SICI)1098-2795(200003)55:3<282::AID-MRD6>3.0.CO;2-7
Bucak M N, Atessahin A and Yuce A. 2007. Effect of anti-oxidants and oxidative stress parameters on ram semen after the freeze– thawing process. Small Ruminant Research 75: 128–34 DOI: https://doi.org/10.1016/j.smallrumres.2007.09.002
Bucak M N and Tekin N. 2007. Protective effect of taurine, glutathione and trehalose on the liquid storage of ram semen. Small Ruminant Research 73: 103–08. DOI: https://doi.org/10.1016/j.smallrumres.2006.12.001
Deka B C, Biswas R K, Nath K C and Barua P M. 2012. Effect of washing on quality of frozen semen in Assam local, Beetal and Beetal × Assam local goats. Compendium of National Symposium on Addressing Animal Reprodructive Stressses through Biotechnological Tools and 28th annual convention of Animal Reproduction, Pp–187.
El–Kishk M A. 2010. ‘Studies on freezing of bovine bull semen.’ Ph .D Thesis, Faculty of Agriculture, Mansoura University, Egypt.
Foote R H, Brockett C C and Kaproth M T. 2002. Motility and fertility of bull sperm in whole milk extender containing antioxidants. Animal Reproduction Science 71: 13–23. DOI: https://doi.org/10.1016/S0378-4320(02)00018-0
Gadea J, Selles E, Marco M A, Coy P, Matas C, Romar R and Ruiz S. 2004. Decrease in glutathione content in boar sperm after cryopreservation. Effect of addition of reduced glutathione to freezing and thawing extenders. Theriogenology 62: 690–701. DOI: https://doi.org/10.1016/j.theriogenology.2003.11.013
Irvine D S. 1996. Gluthatione as a treatment for male infertility. Reviews of Reproduction 1: 6–12. DOI: https://doi.org/10.1530/ror.0.0010006
Kim J G and Parthasarathy S. 1998. Oxidation and spermatozoa. Reproduction and Endocrinology 16 (4): 235–39. DOI: https://doi.org/10.1055/s-2007-1016283
Revel S G and Mrode R A. 1994. An osmotic resistance test for bovine semen. Animal Reproduction Science 36: 77–86. DOI: https://doi.org/10.1016/0378-4320(94)90055-8
Saraswat S, Jindal S K, Ramachandran N, Yadav S, Rout P K, Kharche S D and Goel A K. 2012. The effect of antioxidants supplementation to cryopreservation protocol on seminal attributes and sperm membrane characteristics in Sirohigoat. Journal of Physiology and Pharmacology Advances 2 (1): 49–58.
Sharma S, Sharma N K, Sinha N K and Sharma S S. 2013. Assessment of transaminases and effect of freezing rates on their leakage into seminal plasma of sirohi bucks. Animal and Veterinary Sciences 1(2): 18–22 DOI: https://doi.org/10.11648/j.avs.20130102.12
Sinha M P, Sinha A K, Singh B K and Prasad R L. 1996. The effect of glutathione on the motility, enzyme leakage and fertility of frozen goat semen. Animal Reproduction Science 41: 237–43. DOI: https://doi.org/10.1016/0378-4320(95)01450-0
Slaweta R and Laskowska T. 1987. The effect of glutathione on the motility and fertility of frozen bull semen. Animal Reproduction Science 13: 249–53. DOI: https://doi.org/10.1016/0378-4320(87)90061-3
Tamuli M K and Watson P F. 1994. Use of a simple staining technique to distinguish acrosomal changes in the live sperm sub-population. Animal Reproduction Science 35: 247– 50. DOI: https://doi.org/10.1016/0378-4320(94)90040-X
Veerabramhaiah K, Rao S A, Rao V H, Naidu K V and Rao S T V. 2011. Effect of Tris and Biociphos-plus extenders on the extracellular enzyme release of phosphatases and transferases in Punganur bull semen. Journal of Advanced Veterinary Research 1: 61–64.
Uysal O and Bucak M N. 2007. Effect of oxidized glutathione, bovine serum albumin, cysteine and lycopene on the quality of frozen-thawed buck semen. Acta Veterinaria Brno 76: 383– 39. DOI: https://doi.org/10.2754/avb200776030383
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