Prediction of buffalo bull fertility on the basis of sperm motion traits, viability, membrane integrity, heat shock protein (HSP70) expression and fertility associated antigen (FAA)

AJEET KUMAR; JAGIR SINGH; G V P P S RAVI KUMAR; RANJANA S CHEEMA; A K PANDEY; PAWAN SINGH; S P S GHUMAN; P S BRAR; V K GANDOTRA

doi:10.56093/ijans.v86i6.59160

Authors

AJEET KUMAR Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
JAGIR SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
G V P P S RAVI KUMAR IVRI, Bareilly
RANJANA S CHEEMA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
A K PANDEY LLRVASU, Hisar
PAWAN SINGH NDRI, Karnal
S P S GHUMAN Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
P S BRAR Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
V K GANDOTRA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v86i6.59160

Keywords:

Buffalo bulls, Fertility associated antigen, Fertility prediction, HSP70, Membrane integrity, Sperm motion traits, Viability

Abstract

The present study was conducted on 20 buffalo bulls to predict the fertility on the basis of: (a) Computer assisted semen analysis (CASA) based sperm motion traits, (b) viability, (c) membrane integrity, (d) expression for HSP70, and (e) assessment of fertility associated antigen (FAA). Six frozen semen straws from each buffalo bulls were analyzed for sperm motion traits, viz. individual motility, progressive motility, average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head deviation (ALH), beat cross frequency (BCF), straightness (STR), linearity (LIN) and sperm size. Viability and hypoosmotic swelling tests (HOST) were also conducted. Heat shock protein 70 (HSP70) expression and quantification was conducted using a real time PCR. Fertility associated antigen (FAA) was assessed in fresh semen from buffalo bulls using test. Fertility trial was conducted on 250 normal cycling buffaloes following estrus synchronization with GnRH-PGF- GnRH protocol. It was concluded that buffalo bull fertility could be best predicted on the basis of sperm motion traits (VCL, VAP, VSL, ALH, LIN), motility, viability, HOST and HSP70 expression. However, FAA assessment in fresh semen did not indicate fertility.

Downloads

Download data is not yet available.

References

Anilkumar R, Devanathan T G, Pattabiraman S R and Edwin M J. 2001. Correlation between the spermatozoal characteristics and sperm penetration distance in polyacrylamide gel and bovine cervical mucus. Theriogenology 55 (2): 685–91. DOI: https://doi.org/10.1016/S0093-691X(01)00436-8

Arman C, Quintana Casares P I, Sanchez-Partida L G and Setchell B P. 2006. Ram sperm motility after intermittent scrotal insulation evaluated by manual and computer-assisted methods. Asian Journal of Andrology 8 (4): 411–18. DOI: https://doi.org/10.1111/j.1745-7262.2006.00145.x

Asquith K L, Harman A J, Mclaughlin E A, Nixon B and Aitken R J. 2005. Localization and significance of molecular chaperones, heat shock protein 1 and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction. Biology of Reproduction 72: 328–37. DOI: https://doi.org/10.1095/biolreprod.104.034470

Bellin M E, Oyarzo J N, Hawkins H E, Zhang H, Smith R G, Forrest D W, Sprott L R and Ax R L. 1998. Fertility-associated antigen on bull sperm indicates fertility potential. Journal of Animal Science 76: 2032–39. DOI: https://doi.org/10.2527/1998.7682032x

Dalton J C, Nadir S, Noftsinger M and Saacke R G. 2010. Insemination related factors affecting fertilization in estrus synchronized cattle. Proceedings of Applied Reproductive Strategies in Beef Cattle held at San Antonio, TX from January 28–29.

Dogan S, Mason MC, Govindaraju A, Belser L, Kaya A, Stokes J, Rowe D and Memili E. 2013. Interrelationships between apoptosis and fertility in bull sperm. Journal of Reproduction and Development 59: 18– 26. DOI: https://doi.org/10.1262/jrd.2012-068

Fukuda A, Osawa T, Oda H, Tanaka T, Toyokuni S and Ucbida K. 1996. Oxidative stress response in iron induced acute nephrotoxicity: enhanced expression of heat shock protein 90. Biochemical and Biophysical Research Communications 219: 76–81. DOI: https://doi.org/10.1006/bbrc.1996.0184

Holt C, Holt W V, Moore H D M, Reed H C B and Curnock R M. 1997. Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. Journal of Andrology 18: 312–23.

Irvine D S, Macleod I C, Templeton A A, Masterton A and Taylor A. 1994. A prospective clinical study of the relationship

between the computer-assisted assessment of human semen quality and the achievement of pregnancy in vivo. Human Reproduction 9: 2324–34. DOI: https://doi.org/10.1093/oxfordjournals.humrep.a138446

Kamaruddin M, Kroetsch T, Basrur P K and King W A. 1996. Heat shock protein 70 in bovine semen. Biology of Reproduction 54 (Suppl.): 112.

Kathiravan P, Kalatharan J, Edwinb M J and Veerapandian C. 2008. Computer automated motion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona- free hamster oocytes. Animal Reproduction Science 104: 9– 17. DOI: https://doi.org/10.1016/j.anireprosci.2007.01.002

Kumar A, Singh J, Nanda A S, Pangaonkar G R. 2004. Quality and fertility of post thaw sephadex filtered bull semen. Asian Australasian Journal of Animal Sciences 17 (6): 755–59. DOI: https://doi.org/10.5713/ajas.2004.755

Lewis S E, Donnelly E T, Sterling E S, Kennedy M S, Thompson W and Chakravarthy U. 1996. Nitric oxide synthase and nitrite production in human spermatozoa: evidence that endogenous nitric oxide is beneficial to sperm motility. Molecular Human Reproduction 2: 873–78. DOI: https://doi.org/10.1093/molehr/2.11.873

McCauley T C, Dawson G R, Oyarzo J N, McVicker J, Marks S F and Ax R L. 2004. Development and validation of a lateral- flow cassette for fertility diagnosis in bulls. IVD Technology 10: 35–40.

Muino R, Rivera M M, Rigau T, Rodriguez-Gil J E and Pena A I. 2008. Effect of different thawing rates on post-thaw sperm viability, kinematic parameters and motile sperm subpopulations structure of bull semen. Animal Reproduction Science 109: 50–64. DOI: https://doi.org/10.1016/j.anireprosci.2007.11.028

Mukherjee A, Goswami S L and Mukherjee S. 2006. Assessment of fertilizing potential of bull spermatozoa by computer assisted semen analyzer (CASA). Proceeding of 22nd Annual convention and national symposium on Innovative technologies for fertility enhancement in livestock, held at College of Veterinary Science, Mhow, MP from Nov 10–12. Pp 159.

Park Y J, Kwon W S, Oh S A and Pang M G. 2012. Fertility- related proteomic profiling bull spermatozoa separated by percoll. Journal of Proteome Research 11: 4162– 68. DOI: https://doi.org/10.1021/pr300248s

Parrish J J, Krogenaes J L, Susko A and Parrish J L. 1995. Effect of bovine sperm separation by either swim-up or Percoll method on success of in vitro fertilization and early embryonic development. Theriogenology 44: 859–69. DOI: https://doi.org/10.1016/0093-691X(95)00271-9

Pawar H N, Kumar G V P P S R, Narang R and Agrawal R K. 2014. Heat and cold stress enhances the expression of heat shock protein 70, heat shock transcription factor 1 and cytokines (IL–12, TNF- and GMCSF) in buffaloes. International Journal of Current Microbiology and Applied Sciences 3 (2): 307–17.

Petrunkina A M, Waberski D, Gunzel-Apel A R and Topfer- Petersen E. 2007. Determinants of sperm quality and fertility in domestic species. Reproduction 134: 3–17. DOI: https://doi.org/10.1530/REP-07-0046

Quintero M A, Rigau T and Rodriguez G J E. 2004. Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology 61: 673–90. DOI: https://doi.org/10.1016/S0093-691X(03)00248-6

Sellem E, Broekhuijse M L, Chevrier L, Camugli S, Schmitt E, Schibler L and Koenen E P. 2015. Use of combinations of in vitro quality assessments to predict fertility of bovine semen. doi: 10.1016/j.theriogenology.2015.07.035. DOI: https://doi.org/10.1016/j.theriogenology.2015.07.035

Shi Y, Mosser D D and Morimoto R I. 1998. Molecular chaperones as HSF1-specific transcriptional repressors. Genes and Development 12: 654–66. DOI: https://doi.org/10.1101/gad.12.5.654

Spinaci M, Volpe S, Bernardini C, Ambrogi M D, Tamanini C, Seren E and Galeati G. 2005. Immunolocalization of heat shock protein70 (HSP70) in boar spermatozoa and its role during fertilization. Molecular Reproduction and Development 72: 534–41. DOI: https://doi.org/10.1002/mrd.20367

Zodinsanga V, Mavi P S, Cheema R S, Kumar A and Gandotra V K. 2015. Relationship between routine analysis/sperm function and fertility tests of cattle bull semen. Asian Journal of Animal Sciences 9 (1): 37–44. DOI: https://doi.org/10.3923/ajas.2015.37.44