Optimization of single step multiplex PCR for detection of Eimeria tenella and Eimeria necatrix from commercial broilers

MUHAMMAD UMAIR KHAN; SAHER ISLAM; AMNA ARSHAD BAJWA; IMRAN RASHID; RAHEELA AKHTAR; HAROON AKBAR; WASIM SHEHZAD; MUHAMMAD LATEEF

doi:10.56093/ijans.v86i6.59201

Authors

MUHAMMAD UMAIR KHAN University of Veterinary and Animal Sciences, Lahore 54000 Pakistan
SAHER ISLAM University of Veterinary and Animal Sciences, Lahore 54000 Pakistan
AMNA ARSHAD BAJWA University of Veterinary and Animal Sciences, Lahore 54000 Pakistan
IMRAN RASHID University of Veterinary and Animal Sciences, Lahore 54000 Pakistan
RAHEELA AKHTAR University of Veterinary and Animal Sciences, Lahore 54000 Pakistan
HAROON AKBAR University of Veterinary and Animal Sciences, Lahore 54000 Pakistan
WASIM SHEHZAD University of Veterinary and Animal Sciences, Lahore 54000 Pakistan
MUHAMMAD LATEEF University of Veterinary and Animal Sciences, Lahore 54000 Pakistan

https://doi.org/10.56093/ijans.v86i6.59201

Keywords:

Broiler chicken, Eimeria necatrix, Eimeria tenella, Multiplex PCR

Abstract

Multiplex polymerase chain reaction (PCR) was optimized for detection of 2 important species of Eimeria (Eimeria tenella and E. necatrix). This optimized protocol was used to screen 100 fecal samples collected from broiler birds in Lahore periphery. Out of 100 samples we found 6 tenella and only 1 E. necatrix positive by this method. The detection limit of oocysts by this method was as leastas 16 oocysts. This optimized multiplex PCR method can be used as routine diagnostic tool for detection of E. tenella and E. necatrix and can be extended up to the detection of 7 Eimeria species in future.

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