Effect of vegetable seed oils on methane emission and fermentation of feed in vitro

CHETNA MAHAJAN; KAJAL MANGAL; M WADHWA

doi:10.56093/ijans.v86i7.59963

Authors

CHETNA MAHAJAN Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
KAJAL MANGAL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
M WADHWA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v86i7.59963

Keywords:

Alternate hydrogen sink, Methane mitigation in-vitro, Vegetable seed oils

Abstract

A study was conducted to assess the effect of carrot seed oil, canola seed oil and rape seed oil on rumen fermentation and methanogenesis in-vitro. The oils were supplemented to the complete feed (oat fodder and concentrate mixture in 60: 40 ratio) @ 1, 2 and 3% on DM basis, incubated for 24 h in 100 ml glass syringes. The digestibility of NDF varied significantly with highest in rapeseed and lowest in carrot seed, while OM digestibility and ME availability did not show any significant differences amongst the supplemented oils. The in-vitro methane production from complete feed supplemented with rape seed oil was observed to be the lowest. The TVFAs, acetate and propionate levels were highest in canola oil while A: P ratio was lowest.

TVFA and acetate levels were highest at 1% level of supplementation, whereas propionate level was highest in control followed by at 1% supplementation. The methane production was significantly higher in oil supplemented groups as compared to control group, but amongst the oil supplemented groups it was significantly lowest when oil was supplemented at 3% level. Amongst the various oils evaluated for in vitro methane mitigation, the study conclusively revealed that the supplementation of diet with rape seed oil @ 2–3% on dry matter basis had an edge over other oils and levels.

Downloads

Download data is not yet available.

References

AOAC. 2000. Official Methods of Analysis. 7th edn. Association of Analytical Chemists, Gaithersburg, Maryland, USA.

Bakshi M P S and Wadhwa M. 2009. Dietary manipulation for mitigation of enteric methane emission. Lead paper 5th Asian Buffalo Conference, Lahore. Proceedings in Pakistan Journal of Zoology 9: 887–93. (Environ-SPJZ–2).

Bakshi M P S and Wadhwa M. 2010. Significance of plant secondary metabolites in relation to methane produced by ruminants. Proceedings in Animal Nutrition Association Conference, held at NAASC complex, New Delhi. Vol I (Lead papers). pp 25–28.

Bakshi M P S and Wadhwa M. 2011. Phytogenics: Role in enteric methane mitigation and performance of animals. Nutritional interventions for clean and green livestock production. CAS in Animal Nutrition, IVRI, Izatnagar. pp 180–84.

Bakshi M P S and Wadhwa M. 2012. Herbal feed additives- Role in Animal Nutrition. Animal Nutrition-Advances and Developments. (Eds) Mehra U R, Singh P and Verma A K. pp 707–33. Satish Serial Publishing House, New Delhi.

Beauchemin K A, Kreuzer M, O’Mara F and McAllister T A. 2008. Nutritional management for enteric methane abatement: a review. Australian Journal of Experimental Agriculture 48: 21–27. DOI: https://doi.org/10.1071/EA07199

Benchaar C, Pomar C, Chiquette J. 2001. Evaluation of diet strategies to reduce methane production in ruminants: a modelling approach. Canadian Journal of Animal Science 81: 563–74. DOI: https://doi.org/10.4141/A00-119

Blaxter K L and Clapperton J L. 1965. Prediction of the amount of methane produced by ruminants. British Journal of Nutrition 19: 511–22. DOI: https://doi.org/10.1079/BJN19650046

Cottyn B G and Boucque C V. 1968. Rapid methods for the gas chromatographic determination determination of volatile acids in rumen fluid. Journal of Agricultural and Food Chemistry 16: 105–07. DOI: https://doi.org/10.1021/jf60155a002

Machmüller A, Soliva C R and Kreuzer M. 2003a. Effect of coconut oil and defaunation treatment on methanogenesis in sheep. Reproductive and Nutritional Development 43: 41–55. DOI: https://doi.org/10.1051/rnd:2003005

Maia M R G, Chaudhary L C, Figueres L and Wallace R J 2007 Metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen. Antonie Van Leeuwenhoek 91: 303–14. DOI: https://doi.org/10.1007/s10482-006-9118-2

McAlister T A, Okine E K, Mathison G W and Cheng K J. 1996. Dietary, Environment and microbiological aspects of methane production in ruminants.Canadian Journal of Animal Science 76: 231–43. DOI: https://doi.org/10.4141/cjas96-035

Menke K H and Steingass H. 1988. Estimation the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Animal Research and Development 28: 7– 55.

Menke K H, Raab L, Salweski A, Steingass H, Fritz D and Scheider W. 1979. The estimation of digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agricultural Science Cambridge 93: 217– 22. DOI: https://doi.org/10.1017/S0021859600086305

Mills J A N, Dijkstra J, Bannink A, Cammell, S B Kebreab, E and France J. 2001. A mechanistic model of whole-tract digestion and methanogenesis in the lactating dairy cow: Model development, evaluation, and application. Journal of Animal Science 79: 1584–97. DOI: https://doi.org/10.2527/2001.7961584x

Morales E R, Espinosa M A M, McKain N and Wallace R J. 2012. Ricinoleic acid inhibits methanogenesis and fatty acid biodehydrogenation in ruminal digesta from sheep and in bacterial cultures. Journal of Animal Science 90: 4943–50. DOI: https://doi.org/10.2527/jas.2011-4670

Robertson J A and Van Soest P J. 1981.The Detergent system of analysis and its application to human food. The Analysis of Dietary Fibre in Food. (Eds) W P T James and O Theander. pp. 123–58. Marcel Dekker Inc., New York.

Snedecor G W and W G Cochran. 1994. Statistical Methods. Oxford and IBH Publications, New Delhi.

SPSS. 2007. Statistical Packages for Social Sciences.Version 16, SPSS Inc., Linois, USA.

Wadhwa M and Bakshi M P S. 2010. Animal Agriculture and Greenhouse Gas Emission: Mitigation Strategies. Vol.I (Lead Papers). (Eds) Pattanaik A K, Verma A K, Kamra D N and Sharma K. p. 119–22.

Wolin M J. 1960. A theoretical rumen fermentation balance. Journal of Dairy Science 43: 1452–59. DOI: https://doi.org/10.3168/jds.S0022-0302(60)90348-9