A comparison of in vitro fertilization potential of sperm obtained from refrigerated (4°C) epididymis and fresh ejaculation of buck under different embryo culture media

DHARMENDRA KUMAR; SALIL KUMAR JAIN; BIKASH CHANDRA SARKHEL

doi:10.56093/ijans.v86i8.60778

Authors

DHARMENDRA KUMAR Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India
SALIL KUMAR JAIN Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India
BIKASH CHANDRA SARKHEL Nanaji Deshmukh Veterinary Science University, Jabalpur, Madhya Pradesh 482 004 India

https://doi.org/10.56093/ijans.v86i8.60778

Keywords:

Culture medium, Epididymis, Goat, In vitro fertilization, Semen

Abstract

The present study assessed the fertilization potential of sperm obtained from refrigerated epididymis and fresh ejaculation of buck under 2 culture media, viz. modified synthetic oviductal fluid (mSOF) and research vitro cleave-blastocyst (RVCL-BLAST) media. In the process, the cauda epididymis of slaughtered buck were immediately collected, pre-processed and refrigerated (4°C) for 24 h. The oocytes recovered from slaughter house ovaries were in vitro matured and fertilized with both semen sources. On analysis of embryonic development, ejaculated group showed significantly higher blastocyst rate than epididymal sperm, however, initial cleavage rates of epididymal group was significantly higher than ejaculated semen, irrespective of culture media. Among the culture media, RVCL-BLAST media was significantly efficient than mSOF in terms of cleavage and blastocysts, irrespective of gametes sources. Despite of lower outcome of blastocyst, cold stored epididymal sperm may be considered as an alternative, potent and viable source of gametes for assisted reproduction in case of endangered or other livestock, if immediate facilities for sperm processing are not available.

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References

Al-Timimi I H S. 2013. Using of epididymal sperms of local iraqi bucks for in vitro fertilization. AL-Qadisiya Journal of Veterinary Medical Science 12 (2): 126–32. DOI: https://doi.org/10.29079/vol12iss2art266

Badr M R and Abdel-Malak M G. 2010. In vitro fertilization and embryo production in dromedary camel using epididymal spermatozoa. Global Veterinaria 4: 271–76.

Ball G D, Leibfried M L, Lenz R W, Ax R L, Bavister B D and First N L. 1983. Factors affecting successful in vitro fertilization of bovine follicular oocytes. Biology of Reproduction 28: 717–25. DOI: https://doi.org/10.1095/biolreprod28.3.717

Blash S, Melican D and Gavin W. 2000. Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology 54: 899–905. DOI: https://doi.org/10.1016/S0093-691X(00)00400-3

Dong Q, Rodenburg S E, Huang C and Vandevoort C A. 2008. Cryopreservation of rhesus monkey (Macaca mulatta) epididymal spermatozoa before and after refrigerated storage. Journal of Andrology 29: 283–92. DOI: https://doi.org/10.2164/jandrol.107.003921

Dragileva E, Rubinstein S and Breitbart H. 1999. Intracellular Ca2+-Mg2+-ATPase regulates calcium influx and acrosomal exocytosis in bull and ram spermatozoa. Biology of Reproduction 61: 1226–34. DOI: https://doi.org/10.1095/biolreprod61.5.1226

Guerrero C A, Gentry G, Saenz J, Bondioli K R and Godke R A. 2008. Birth of calves after artificial insemination with cryopreserved bovine epididymal spermatozoa harvested from postmortem bulls. Reproduction, Fertility and Development 21: 105. DOI: https://doi.org/10.1071/RDv21n1Ab9

Iranpour F G and Valojerdi M R. 2013. The epididymal sperm viability, motility and DNA integrity in dead mice maintained at 4–6°C. Iranian Journal of Reproductive Medicine 11: 195– 200.

Jena M K, Malakar D, De A K, Garg S, Akshey Y S, Dutta R, Sahu S, Mohanty A K and Kaushik J K. 2012. Handmade cloned and parthenogenetic goat embryos-A comparison of different culture media and donor cells. Small Ruminant Research 105: 255–62. DOI: https://doi.org/10.1016/j.smallrumres.2012.03.001

Jimenez-Macedo A R, Izquierdo D, Anguita B and Paramio M T. 2005. Comparison between intracytoplasmic sperm injection and in vitro fertilization employing oocytes derived from prepubertal goats. Theriogenology 64: 1249–62. DOI: https://doi.org/10.1016/j.theriogenology.2004.11.025

Kishikawa H, Tateno H and Yanagimachi R. 1999. Fertility of mouse spermatozoa retrieved from cadavers and maintained at 4°C. Journal of Reproduction and Fertility 116: 217–22. DOI: https://doi.org/10.1530/jrf.0.1160217

Kumar D, Gopalkrishna R, Singh A P, Ranjan R, Pandey S K and Sarkhel B C. 2014. Developmental potency of pre-implant parthenogenetic goat embryos: effect of activation protocols and culture media. In Vitro Cellular & Developmental Biology - Animal 50 (1): 1–6. DOI: https://doi.org/10.1007/s11626-013-9680-7

Leibo S P and Songsasen N. 2002. Cryopreservation of gametes and embryos of non-domestic species. Theriogenology 57: 303–26. DOI: https://doi.org/10.1016/S0093-691X(01)00673-2

Monteiro G A, Papa F O, Zahn F S, Dellaqua Jr J A, Melo C M, Maziero R R D, Avanzi B R, Alvarenga M A and Guasti P N. 2011. Cryopreservation and fertility of ejaculated and epididymal stallion sperm. Animal Reproduction Science 127: 197–201. DOI: https://doi.org/10.1016/j.anireprosci.2011.08.002

Newton L D, Krishnakumar S, Menon A G, Kastelic J P, Van Der Hoorn F A and Thundathil J C. 2010. Na+/K+-ATPase regulates sperm capacitation through a mechanism involving kinases and redistribution of its testis-specific isoform. Molecular Reproduction and Development 77: 136–48. DOI: https://doi.org/10.1002/mrd.21114

Pavlok A, Torner H, Motlik J, Fulka J, Kauffold P and Duschinski U. 1988. Fertilization of bovine oocytes in vitro: Effect of different sources of gametes on fertilization rate and frequency of fertilization anomalies. Animal Reproduction Science 16: 207–13. DOI: https://doi.org/10.1016/0378-4320(88)90014-0

Rath D and Niemann H. 1997. In vitro fertilization of porcine oocytes with fresh and frozen-thawed ejaculated or frozen- thawed epididymal semen obtained from identical boars. Theriogenology 47: 785–93. DOI: https://doi.org/10.1016/S0093-691X(97)00034-4

Santiago-Moreno J, Toledano-Díaz A, Pulido-Pastor A, Dorado J, Gómez-Brunet A and López-Sebastián A. 2006. Effect of egg yolk concentration on cryopreserving Spanish ibex (Capra pyrenaica) epididymal spermatozoa. Theriogenology 66: 1219–26. DOI: https://doi.org/10.1016/j.theriogenology.2006.03.031

Shah R A, George A, Singh M K, Kumar D, Chauhan M S, Manik R, Palta P and Singla S K. 2008. Handmade cloned buffalo embryos: comparison of different media and culture systems. Cloning Stem Cells 10: 435–41. DOI: https://doi.org/10.1089/clo.2008.0033

Snedecor G W and Cochran W G. 1994. Statistical Methods. 7th edn. Oxford and IBH Publishing Co., New Delhi.

Soler A J, Esteso M C, Fernández-Santos M R and Garde J J. 2005. Characteristics of Iberian red deer (Cervus elaphus hispanicus) spermatozoa cryopreserved after storage at 5°C in the epididymis for several days. Theriogenology 64 (7): 1503–17. DOI: https://doi.org/10.1016/j.theriogenology.2005.03.013

Song H B. 1988. In vitro fertilization of goat follicular oocytes with epididymal spermatozoa. Korean Journal of Animal Science 30: 632–42.

Songsasen N, Tong J and Leibo S P. 1988. Birth of live mice derived by in vitro fertilization with spermatozoa retrieved up to twenty-four hours after death. Journal of Experimental Zoology 280: 189–96. DOI: https://doi.org/10.1002/(SICI)1097-010X(19980201)280:2<189::AID-JEZ10>3.0.CO;2-H

Wani A R, Khan M Z, Sofi K A, Lone F A, Malik A A and Bhat F A. 2012. Effect of cysteamine and epidermal growth factor (EGF) supplementation in maturation medium on in vitro maturation, fertilization and culturing of embryos in sheep. Small Ruminant Research 106: 160–64. DOI: https://doi.org/10.1016/j.smallrumres.2012.02.015

Yu I and Leibo S P. 2002. Recovery of motile, membrane-intact spermatozoa from canine epididymides stored for 8 days at 4°C. Theriogenology 57 (3): 1179–90. DOI: https://doi.org/10.1016/S0093-691X(01)00711-7