Impact of body burden of pesticide residues on the reproductive tract of buffalo

KARANPREET KAUR; SARVPREET SINGH GHUMAN; OPINDER SINGH; JASBIR SINGH BEDI; JATINDER PAUL SINGH GILL

doi:10.56093/ijans.v86i9.61557

Authors

KARANPREET KAUR Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
SARVPREET SINGH GHUMAN Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
OPINDER SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
JASBIR SINGH BEDI Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
JATINDER PAUL SINGH GILL Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v86i9.61557

Keywords:

Buffalo, Histopathology, Ovarian follicular fluid, Pesticide residues, Reproductive tract

Abstract

The disruption of reproductive system leading to infertility can occur due to accumulation of endocrine disrupting chemicals such as pesticide residues in the reproductive tract and ovarian follicular fluid after their entry into blood of animals. Infertile adult female buffalo (100) from a slaughterhouse were utilized to assess the presence of pesticide residues in their blood and reproductive tract as well as the microscopic alterations in the tract. The pesticide residues were detected in 25% blood, 43% reproductive tract and 32% ovarian follicular fluid samples by gas chromatograph (GC) and were confirmed by gas chromatograph-mass spectroscope (GC-MS). Compared to blood, the pesticide residues were high in reproductive tract. The concomitant presence of specific pesticide residues was observed in blood, reproductive tract and follicular fluid. Histopathological alterations in pesticide-negative as well as low and highly pesticide-positive reproductive tracts of slaughtered buffaloes were 0.25±0.71, 1.87±2.23 and 3.65±1.68/sample, respectively. The major alterations were leiomyosarcoma, haemangiosarcoma, cystic ovarian degeneration, fibrotic/atrophic ovaries and cyst adenoma. In conclusion, the presence of pesticide residues in the reproductive tract of female buffalo, may be a factor contributing towards infertility.

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References

Aziz F, Mehboob Z, Jalali S, Shami S A and Qureshi I Z. 2012. Effect of organophosphate insecticide Malathion on in-vitro oocyte maturation, fertilization and embryo development of Nili Ravi buffalo (Bubalus bubalis). Journal of Animal and Plant Sciences 22: 147–52.

Bedi J S, Gill J P S, Aulakh R S, Kaur P, Sharma A and Pooni P A. 2013. Pesticide residues in human breast milk: Risk assessment for infants from Punjab, India. Science of The Total Environment 463: 720–26. DOI: https://doi.org/10.1016/j.scitotenv.2013.06.066

Crellin N K, Rodway M R, Swan C L, Gillio-Meina and Chedrese P J. 1999. Dichloro-diphenyldichloroethylene potentiates the effect of protein kinase a pathway activators on progesterone synthesis in cultured porcine granulosa cells. Biology of Reproduction 61: 1099–1103. DOI: https://doi.org/10.1095/biolreprod61.4.1099

Debebe A and Kuttalam S. 2011. Parameter validation of analytical methods of insecticide residue analyses in foods of animal origin, feed and water. Bulletin of Environment Contamination and Toxicology 86: 571–75. DOI: https://doi.org/10.1007/s00128-011-0284-1

Directorate of Economics and Statistics. 2013. Agricultural Statistics at a Glance. Department of Agriculture and Cooperation, Ministry of Agriculture. http://eands.dacnet.nic.in/latest_2006.htm.

Dobrinas S, Stanciu G, Soceanu A and Culea A. 2013. Analysis of organochlorine and pyrethroid pesticide residues in baby food samples. Ovidius University Annals of Chemistry 22: 107– 12.

Dutta H M, Nath A, Adhikari S, Roy P K, Singh N K and Munshi J S D. 1994. Sublethal Malathion induced changes in the ovary of an air-breathing fish, Heteropneustes fossilis: a histological study. Hydrobiologia 294: 215–18. DOI: https://doi.org/10.1007/BF00021294

Faundez R, Sitarska E, Klucinski W and Duszewska A M. 1996. The effect of persistent chlorinated hydrocarbons on the secretion of estradiol and progesterone by bovine granulosa cells in vitro. Zentralblatt fur Veterinarmedizin Reihe A 43: 317–23. DOI: https://doi.org/10.1111/j.1439-0442.1996.tb00458.x

Forsdike R A, Hardy K, Bull L, Stark J, Webber L J, Stubbs S, Robinson J E and Franks S. 2007. Disordered follicle development in ovaries of prenatally androgenized ewes. Journal of Endocrinology 192: 421–28. DOI: https://doi.org/10.1677/joe.1.07097

Fowler P A, Dora N J, McFerran H, Amezaga M, Miller D W, Lea R G, Cash P, McNeilly A S, Evans N P, Cotinot C, Sharpe R M and Rhind S M. 2008. In utero exposure to low doses of environmental pollutants disrupts fetal ovarian development in sheep. Molecular Human Reproduction 14: 269–80. DOI: https://doi.org/10.1093/molehr/gan020

Gill U S, Schwartz H M and Wheatley B. 1996. Development of a method for the analysis of PCB congeners and organochlorine pesticides in blood/serum. Chemosphere 32: 1055–61. DOI: https://doi.org/10.1016/0045-6535(96)00025-2

Heo J H, Lee Y H, Kim G I, Kim T H, Kang H, An H J, Yoon B S, Seong H J, Park H and Kim J Y. 2011. A case of ovarian microinvasive mucinous carcinoma and co-existent angiosarcoma. Korean Journal of Pathology 45: 96–100. DOI: https://doi.org/10.4132/KoreanJPathol.2011.45.1.96

Hernandez-Ochoa I, Karman B N and Flaws J A. 2009. The role of the aryl hydrocarbon receptor in the female reproductive system. Biochemical Pharmacology 77: 547–59. DOI: https://doi.org/10.1016/j.bcp.2008.09.037

Kamel R M. 2010. A massive ovarian mucinous cystadenoma: A case report. Reproductive Biology and Endocrinology 8: 24. DOI: https://doi.org/10.1186/1477-7827-8-24

Kaul R and Sharma J. 2009. Primary leiomyosarcoma of ovary. Online Journal of Health Allied Sciences 8: 16.

Krogenaes A K, Nafstad I, Skare J U, Farstad W and Hafne A L. 1998. In vitro reproductive toxicity of polychlorinated biphenyl congeners 153 and 126. Reproductive Toxicology 12: 575–80. DOI: https://doi.org/10.1016/S0890-6238(98)00040-9

Lerch S, Guidou C, Thomes J P and Jurjanz S. 2016. Non-dioxin- like polychlorinated biphenyls (PCBs) and chlordecone release from adipose tissue to blood in response to body fat mobilization in ewe (Ovis aries). Journal of Agricultural and Food Chemistry 64(5): 1212–20. DOI: https://doi.org/10.1021/acs.jafc.5b05817

Luna L G. 1968. Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology. 3rd edn. McGraw-Hill, New York, NY.

Magnarelli G and Fonovich T. 2013. Protein phosphorylation pathways disruption by pesticides. Advances in Biological Chemistry 3: 460–74. DOI: https://doi.org/10.4236/abc.2013.35050

Mathur V, John P J, Soni I and Bhatnagar P. 2008. Blood levels of organochlorine pesticide residues and risk of reproductive tract cancer among women from Jaipur, India. Advances in Experimental Medicine and Biology 617: 387–94. DOI: https://doi.org/10.1007/978-0-387-69080-3_37

Ratnakaran U, Ghuman S P S, Bedi J S and Gill J P S. 2014. Pesticide residue accumulation in buffalo ovaries: a potential hazard to fertility. Indian Journal of Animal Sciences 84 (7): 741–44.

Sharma K K. 2007. Pesticide Residue Analysis Manual. Indian Council of Agricultural Research (ICAR), New Delhi, India. Silins I and Hogberg J. 2011. Combined toxic exposures and human health: Biomarkers of exposure and effect. International Journal of Environmental Research and Public Health 8: 629–47. DOI: https://doi.org/10.3390/ijerph8030629

Steckler T, Wang J, Bartol F F, Roy S K and Padmanabhan V. 2005. Fetal programming: prenatal testosterone treatment causes intrauterine growth retardation, reduces ovarian reserve and increases ovarian follicular recruitment. Endocrinology 146: 3185–93. DOI: https://doi.org/10.1210/en.2004-1444

Thakur J S, Rao B T, Rajwanshi A, Parwana H K and Kumar R. 2008. Epidemiological study of high cancer among rural agricultural community of Punjab in Northern India. International Journal of Environmental Research and Public Health 5: 399–407. DOI: https://doi.org/10.3390/ijerph5050399

Tiemann U. 2008. In vivo and in vitro effects of the organochlorine pesticides DDT, TCPM, methoxychlor, and lindane on the female reproductive tract of mammals: a review. Reproductive Toxicology 25: 316–26. DOI: https://doi.org/10.1016/j.reprotox.2008.03.002