Expression of the Mir-183 cluster in the follicles and corpus luteum of cattle

TENG-HE MA; LONG CHENG; BIN WANG; GUAN-HUI LIU; LI-NA XU; YING BAI; YU-XIANG SHI

doi:10.56093/ijans.v86i10.62409

Authors

TENG-HE MA Hebei University of Engineering, Handan 056 021 China
LONG CHENG Hebei University of Engineering, Handan 056 021 China
BIN WANG Hebei University of Engineering, Handan 056 021 China
GUAN-HUI LIU Hebei University of Engineering, Handan 056 021 China
LI-NA XU Hebei University of Engineering, Handan 056 021 China
YING BAI Hebei University of Engineering, Handan 056 021 China
YU-XIANG SHI Hebei University of Engineering, Handan 056 021 China

https://doi.org/10.56093/ijans.v86i10.62409

Keywords:

Cattle, Corpus luteum, Follicle, MiR-183 cluster

Abstract

In this study, the expression profiles of the miR-183 cluster were explored in bovine dominant and subordinate follicles and different luteal phases. This miRNA cluster showed higher expression levels in dominant bovine follicles than in subordinates. The expression levels of miR- 182 and miR-96 were reduced from the early phase to the regressed phase, whereas miR-183 showed lower expression levels in the mid and late CL phases than in the early and regressed CL phases.

Downloads

Download data is not yet available.

References

Dai L, Xu J, Liu S, Ma T, Zhu Y, Xu F, Gao Y, Yuan B, Wang S, Zhang Y, Sun G and Zhang J. 2014. Characterization of miR- 126–3p and its target talin2 in the bovine corpus luteum during the oestrus cycle. Reproduction in Domestic Animals 49(6): 913–19. DOI: https://doi.org/10.1111/rda.12400

Gebremedhn S, Salilew-Wondim D, Ahmad I, Sahadevan S, Hossain M M, Hoelker M, Rings F, Neuhoff C, Tholen E, Looft C, Schellander K and Tesfaye D. 2015. MicroRNA expression profile in bovine granulosa cells of preovulatory dominant and subordinate follicles during the late follicular phase of the estrous cycle. PLoS One 10(5): e0125912. DOI: https://doi.org/10.1371/journal.pone.0125912

Livak K J and Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(- Delta Delta C(T)) method. Methods 25 (4): 402–08. DOI: https://doi.org/10.1006/meth.2001.1262

Maalouf S, Smith C and Pate J L. 2016. Changes in microRNA expression during maturation of the bovine corpus luteum: regulation of luteal cell proliferation and function by microRNA-34a. Biology of Reproduction 94(3):71. DOI: https://doi.org/10.1095/biolreprod.115.135053

Maalouf S W, Liu W S, Albert I and Pate J L. 2014. Regulating life or death: potential role of microRNA in rescue of the corpus luteum. Molecular and Cellular Endocrinology 398 (1–2): 78–88. DOI: https://doi.org/10.1016/j.mce.2014.10.005

Miyamoto Y, Skarzynski D J and Okuda K. 2000. Is tumor necrosis factor alpha a trigger for the initiation of endometrial prostaglandin F(2alpha) release at luteolysis in cattle. Biology of Reproduction 62 (5): 1109–15. DOI: https://doi.org/10.1095/biolreprod62.5.1109

Spicer L J, Schreiber N B, Lagaly D V, Aad P Y, Douthit L B and Grado-Ahuir J A. 2011. Effect of resistin on granulosa and theca cell function in cattle. Animal Reproduction Science 124 (1–2): 19–27. DOI: https://doi.org/10.1016/j.anireprosci.2011.01.005