Electrophoretic profile of sperm membrane proteins and correlation with fresh semen characteristics in Assam Hill goat

S DEORI; B C DEKA; A ARANGASAMY; R K BISWAS; S SELVARAJU; D BHUYAN; N NAHARDEKA; L SOMASHEKAR; S PARTHIPAN; D J KALITA; RUMI SAIKIA BORAH; ARUNDHATI PHOOKAN

doi:10.56093/ijans.v86i11.63001

Authors

S DEORI ICAR-NRC on Yak, Dirang, Arunachal Pradesh
B C DEKA Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
A ARANGASAMY Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
R K BISWAS Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
S SELVARAJU Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
D BHUYAN Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
N NAHARDEKA Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
L SOMASHEKAR Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
S PARTHIPAN Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
D J KALITA Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
RUMI SAIKIA BORAH Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India
ARUNDHATI PHOOKAN Assam Agricultural University, Khanapara, Guwahati, Asom 781 022 India

https://doi.org/10.56093/ijans.v86i11.63001

Keywords:

Correlation, Electrophoresis, Goat, Semen characteristics, Sperm membrane protein

Abstract

An experiment was carried out to study the electrophoretic profile of sperm membrane proteins in Assam Hill goat (AHG) bucks and their correlation with fresh semen characteristics. Ejaculates (64) consisting of 8 each from 8 bucks were collected by artificial vagina method. Each ejaculate was split into 2 equal parts. First part was used for fresh semen evaluation and the other part for protein study. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS–PAGE) indicated 20 different protein bands with molecular weight ranging from 10 kDa to 75 kDa. Among the 20 proteins, only 6 proteins bands were consistently present in all 8 bucks (100%). Protein bands with molecular weight 10, 14, 22, 28, 55, 57 and 60 kDa showed significant positive correlation with certain parameters of the fresh semen, while 47 kDa protein band presented negative correlation with fresh semen characteristics in AHG bucks. In conclusion, the proteins showing significant positive correlation with fresh semen characteristics might serve to screen semen of AHG bucks for breeding programmes.

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