Cryosurvival of Marwari stallion sperm in different extenders

TEJPAL TEJPAL; J S MEHTA; S K RAVI; T R TALLURI; ASHOK KUMAR; AMIT KUMAR; YOGESH SONI

doi:10.56093/ijans.v86i12.65961

Authors

TEJPAL TEJPAL College of Veterinary and Animal Sciences, Bikaner, Rajasthan 334 001 India
J S MEHTA College of Veterinary and Animal Sciences, Bikaner, Rajasthan 334 001 India
S K RAVI College of Veterinary and Animal Sciences, Bikaner, Rajasthan 334 001 India
T R TALLURI ICAR-National Research Centre on Equines, Bikaner
ASHOK KUMAR College of Veterinary and Animal Sciences, Bikaner, Rajasthan 334 001 India
AMIT KUMAR College of Veterinary and Animal Sciences, Bikaner, Rajasthan 334 001 India
YOGESH SONI College of Veterinary and Animal Sciences, Bikaner, Rajasthan 334 001 India

https://doi.org/10.56093/ijans.v86i12.65961

Keywords:

Cryopreservation, Extender, Marwari horse, Semen

Abstract

Extenders (3) were compared for frozen-thawed semen quality of Marwari horses in the present study. Ejaculates (36) including 6 ejaculates from each of 6 stallions were collected using artificial vagina (AV) method. The ejaculates were cryopreserved either with glucose-EDTA-lactose, HF-20 or egg yolk-skim milk extender using custom freezing technique. Sperm characteristics, viz. motility, viability, acrosome intactness and plasma membrane integrity were studied in frozen-thawed semen besides record of fresh semen quality as a prerequisite. Present study indicated a non-significant difference in frozen-thawed semen parameters with glucose-EDTA-lactose and HF-20 extenders, however, both the extenders resulted in a higher percentage of post-thaw motility (PTM), viability, intact acrosome and plasma membrane integrity of sperm than the egg yolk-skim milk extender. It was concluded that glucose-EDTA-lactose and HF-20 are better semen extenders as compared to egg yolk-skim milk and they can be used effectively for semen cryopreservation in horses.

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