Relationship between sperm penetration distance in cervical mucus and frozen semen characteristics vis-à-vis buffalo bull fertility

A K SINGH; P S BRAR; RANJNA S CHEEMA

doi:10.56093/ijans.v86i12.65964

Authors

A K SINGH Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
P S BRAR Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
RANJNA S CHEEMA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v86i12.65964

Keywords:

Buffalo bull, Cervical mucus, Fertility, Penetration distance, Sperm characteristics

Abstract

In this study, relationship of sperm penetration distance in mucus was assessed and compared with spermatozoal characteristics, utilizing frozen semen samples obtained from 30 buffalo bulls. Quality semen parameters (motility, kinematics, morphology, viability, HOST, acrosomal and DNA integrity, acrosome reaction) in conjunction with first service conception rate (FSCR) of bulls were determined. Based on penetration distance in buffalo mucus, semen samples were divided into 2 groups, viz. greater penetration distance (GPD, > 20 mm; n, 15) and lesser penetration distance (LPD, < 20 mm; n, 15). Most spermatozoal characteristics were significantly (P<0.05) higher in GPD than in LPD group. The spermatozoa of GPD and LPD were significantly and positively correlated with total motility, progressive motility, acrosome integrity and acrosome reaction. Alternatively, a negative relationship was found with percentage of abnormal spermatozoa and damaged membrane integrity (HOST) in the two penetration distances. In conclusion, significant positive correlations occurred between sperm penetration distance, semen characteristics and fertility parameters. A combination of factors must, therefore, be used to predict the bull fertility.

In conclusion, significant positive correlations existed between sperm penetration distance, quality semen characteristics and fertility parameters. The distance penetrated by spermatozoa capable of going beyond 20 mm in an optimized sperm migration test, could be a useful parameter for in vitro evaluation of sperm quality and subsequent fertility of buffalo bulls.

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References

Anilkumar R, Devanathan T G, Pattabiraman S R and Edwin M J. 2001. Correlation between the spermatozoal characteristics and sperm penetration distance in polyacrylamide gel and bovine cervical mucus. Theriogenology 55: 685–91. DOI: https://doi.org/10.1016/S0093-691X(01)00436-8

Gillan L, Kroetsch T, Maxwell W M and Evans G. 2008. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Animal Reproduction Science 103: 201– 14. DOI: https://doi.org/10.1016/j.anireprosci.2006.12.010

Hollinshead F K, Gillan L, O’Brien J K, Evans G and Maxwell W M C. 2003. In vitro and in vivo assessment of functional capacity of flow cytometrically sorted ram spermatozoa after freezing and thawing. Reproduction Fertility and Development 15: 351–59. DOI: https://doi.org/10.1071/RD03060

Ivic A, Onyeaka H, Girling A, Brewis I A, Ola B and Hammadieh N. 2002. Critical evaluation of methylcellulose as an alternative medium in sperm migration tests. Human Reproduction 17: 143–49. DOI: https://doi.org/10.1093/humrep/17.1.143

Lui D Y and Baker H W. 1992. Sperm nuclear chromatin normality: relationship with sperm morphology, sperm-zona pellucida binding and fertilization rates in vitro. Fertility and Sterility 58: 1178–84. DOI: https://doi.org/10.1016/S0015-0282(16)55566-6

Martinez-Pastor F, Fernandez-Santos M R, del Olmo E, Dominguez-Rebolledo A E, Esteso M C and Montoro V. 2008. Mitochondrial activity and forward scatter vary in necrotic, apoptotic and membrane-intact spermatozoan subpopulations. Reproduction Fertility and Development 20: 547–56. DOI: https://doi.org/10.1071/RD08002

Martinez-Rodriguez C, Alvareza M, Ordasa L, Chamorroa C A, Martinez-Pastora F, Anela L and de Paza P. 2012. Evaluation of ram semen quality using polyacrylamide gel instead of cervical mucus in the sperm penetration test. Theriogenology 77: 1575–86. DOI: https://doi.org/10.1016/j.theriogenology.2011.11.026

Muino-Blanco T, Perez-Pe R and Cebrian-Perez J A. 2008. Seminal plasma proteins and sperm resistance to stress. Reproduction in Domestic Animals 43: 18–31. DOI: https://doi.org/10.1111/j.1439-0531.2008.01228.x

Ola B, Afnan M, Papaioannou S, Sharif K, Erndahl L B and Coomarasamy A. 2003. Accuracy of sperm-cervical mucus penetration tests in evaluating sperm motility in semen: a systematic quantitative review. Human Reproduction 18(5): 1037–46. DOI: https://doi.org/10.1093/humrep/deg209

Phillips N J, McGowan M R, Johnston S D and Mayer D G. 2004. Relationship between 30 post-thaw spermatozoal characteristics and the field fertility of 11 high-use Australian AI sires. Animal Reproduction Science 81: 47–61. DOI: https://doi.org/10.1016/j.anireprosci.2003.10.003

Tas M, Bacinoglu S, Cirit U, Ozdas O B and Kemal A K. 2007. Relationship between bovine fertility and the number of spermatozoa penetrating the cervical mucus within straws. Animal Reproduction Science 101: 18–27. DOI: https://doi.org/10.1016/j.anireprosci.2006.08.020

Ulcova-Gallova Z. 2010. Immunological and physicochemical properties of cervical ovulatory mucus. Journal of Reproductive Immunology 86: 115–21. DOI: https://doi.org/10.1016/j.jri.2010.07.002

Verberckmoes S, Van Soom A, De Pauw I, Dewulf J and De Kruif A. 2002. Migration of bovine spermatozoa in synthetic medium and its relation to in vivo bull fertility. Theriogenology 58: 1027–37. DOI: https://doi.org/10.1016/S0093-691X(02)00931-7

Yanagimachi R. 1994. Mammalian Fertilization. Physiology of Reproduction. 2nd edn, pp. 189–317. (Eds) Knobil E and Neill J D. Raven Press, New York.

Zodinsanga V, Mavi P S, Cheema R S, Kumar A and Gandotra V K. 2015. Relationship between routine analysis/sperm function and fertility tests of cattle bull semen. Asian Journal of Animal Sciences 9(1): 37–44. DOI: https://doi.org/10.3923/ajas.2015.37.44