Evaluation of salivary biomolecules as an effective indicator in accurate and precised identification of estrus in Murrah buffaloes for timed AI

INDU DEVI; PAWAN SINGH; S S LATHWAL; YAJUVENDRA SINGH; KULDEEP DUDI; ANJALI KUMARI

doi:10.56093/ijans.v86i12.65971

Authors

INDU DEVI ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
PAWAN SINGH ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
S S LATHWAL ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
YAJUVENDRA SINGH CVSc & AH, DUVASU, Mathura
KULDEEP DUDI Animal Nutrition Group, NDDB, Chandigarh
ANJALI KUMARI ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v86i12.65971

Keywords:

Heat detection, Phases of estrous cycle, Salivary composition, Timed AI

Abstract

The present study was designed to estimate changes in salivary pH, electrical conductivity (EC) and its various constituent biomolecules like total carbohydrates, total proteins, albumin, mucin 5 and total lipids, during different phases of estrous cycle. In present investigation, the levels of all biomolecules like total CHO, total proteins, albumin, mucin 5, total lipids and EC were observed to be significantly (P<0.01) higher during estrus phase compared to all other phases. No significant difference was found in pH of saliva during different phases of estrous cycle. These changes may be helpful in explaining the relationship between characteristics of saliva and ovarian hormones viz. preovulatory estrogen surge. Thus, variation in salivary composition during different phases of estrous cycle may be helpful in accessing the specific phase of estrous cycle and carrying out the timed AI programme more precisely.

Downloads

Download data is not yet available.

References

Alagendran S, Devi C A, Karthikeyan K, Arulmozhi N and Pushpa N. 2009. Evaluation of thyroid profile in human saliva with special reference to ovulation. Research Journal of Medical Sciences 4: 441–45. http://insipub.net/rjmms/2009/441–445.pdf.

Alagendran S, Saibaba G, Muthukumar S, Rajkumar R, Guzman R G and Archunan G. 2013. Characterization of salivary protein during ovulatory phase of menstrual cycle through MALDITOF/MS. Indian Journal of Dental Research 24: 157–63. DOI: https://doi.org/10.4103/0970-9290.116669

Al-Khafagy R M, Al-Ani S H and Majid A Y. 2012. Ovulation detection through salivary levels of sialic acid and

glycosaminoglycans. Journal of Bangladesh College of Dentistry 24: 66–69. DOI: https://doi.org/10.3329/jbcps.v24i2.151

Bailey C B and Balch C C. 1961. Saliva secretion and its relation to feeding in cattle. 2. The composition and rate of secretion of mixed saliva in the cow during rest. British Journal of Nutrition 15: 383–402. DOI: https://doi.org/10.1079/BJN19610048

Beynon R J and Hurst J L. 2004. Scent wars the chemical biology of competitive signalling in mice. Biological Essays 26: 1288–98. DOI: https://doi.org/10.1002/bies.20147

Calamera J C, Vilar O and Nicholson R. 1986. Changes in sialic acid concentration in human saliva during the menstrual cycle. International Journal of Fertility 31: 43–45.

GoI. 2013–14. Department of Animal Husbandry Dairying and Fisheries. Government of India.

Gandara B K, Leresche L and Mancl L. 2007. Patterns of salivary estradiol and progesterone across the menstrual cycle. Annals of New York Academy of Sciences 1098: 446–50. DOI: https://doi.org/10.1196/annals.1384.022

Glencross R G and Pope G S. 1989. Concentration of estradiol 17 and progesterone in the plasma of dairy heifers before

and after cloprostenol induced and natural luteolysis and during early pregnancy. Animal Reproduction Science 4: 93–106.

Kannan S and Archunan G. 2001. Chemistry of clitoral gland secretions of the laboratory rat: assessment of behavioural DOI: https://doi.org/10.1007/BF02703648

response to identified compounds. Journal of Bioscience 26: 247–52.

Mondal S, Suresh K P and Nandi S. 2010. Endocrine profile of oestrous cycle in buffaloes: a metaanalysis. Asian Australian

Journal of Animal Science 23: 169–74.

Rameshkumar K and Archunan G. 2006. Analysis of urinary fatty acids in bovine (Bos taurus): An effective method for estrus detection. Indian Journal of Animal Sciences 76: 9.

Suthar V S and Dhami A J. 2010. Estrus detection methods in buffalo. Veterinary World 3: 94– 96.

Suthanthirakannan R and Rameshkumar K. 2014. Quantitative analysis of biochemical and hormones in bovine saliva (Bos

indicus) with reference to estrus detection. Scitech Journal 1: 26–29.

Rao T K S, Kumar N, Kumar P, Chaurasia S and Patel N B. 2013. Heat detection techniques in cattle and buffalo. Veterinary DOI: https://doi.org/10.5455/vetworld.2013.363-369

World 6: 363–69.