In vitro effect of copper treatments on mitogenic response and superoxide dismutase expression of lead exposed leukocytes from periparturient dairy cows

MUNEENDRA KUMAR; HARJIT KAUR; BHUPENDRA TULSIDAS PHONDBA; VEENA MANI; NEELAM GUPTA; AMRISH KUMAR TYAGI; RAJU KUSHWAHA; GULAB CHANDRA

doi:10.56093/ijans.v86i12.66004

Authors

MUNEENDRA KUMAR College of Veterinary Science and Animal Husbandry, DUVASU, Mathura
HARJIT KAUR Agricultural Extension Division, KAB-I, ICAR, New Delhi
BHUPENDRA TULSIDAS PHONDBA ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
VEENA MANI ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India
NEELAM GUPTA National Bureau of Animal Genetic Resources, Karnal, Haryana 131 002 India
AMRISH KUMAR TYAGI National Bureau of Animal Genetic Resources, Karnal, Haryana 131 002 India
RAJU KUSHWAHA College of Veterinary Science and Animal Husbandry, DUVASU, Mathura
GULAB CHANDRA College of Veterinary and Animal Sciences, SVBPUAT, Meerut

https://doi.org/10.56093/ijans.v86i12.66004

Keywords:

Copper, Lead, Lymphocytes proliferation, Periparturient cows, Superoxide dismutase

Abstract

The aim of this study was to evaluate whether copper (Cu) treatment in lead (Pb)-exposed lymphocytes can modulate their proliferation and superoxide dismutase (SOD) expression. Blood samples were collected from advanced pregnant crossbred dairy cows at –30, –15, 0, 15, and 30 days of calving and evaluated for lymphocytes proliferation and mRNA expression of SOD. A fixed number of lymphocytes (2×106) were cultured for 72 h with 10–4, 10–5, and 10–6 molar (M) levels of Pb. Pb-exposed lymphocytes were treated with 30, 35 and 40 micro molar (µM) Cu. Mitogenic response of lymphocytes and mRNA expression of SOD reduced as the days of parturition advanced and were noted to be lowest at the day of calving. Reduced mitogenic response of lymphocytes and mRNA expression of SOD was reported in Pb-treated groups as compared to control. Addition of Cu in the Pb- exposed lymphocytes culture improved lymphocytes proliferation and relative mRNA SOD expression. Lymphocytes proliferation and relative mRNA SOD expression was reported highest in 40 µM Cu-treated groups. These results indicated that Cu can ameliorate the adverse effects of Pb on lymphocytes proliferation and SOD expression in periparturient dairy cows.

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References

Aboud A S A. 2010. Impact of pollution with lead, mercury and cadmium on the immune response of Oreochromis niloticus Omima 30 uM Cu. New York Science Journal 3(9): 12–16.

Adonaylo V N and Oteiza P I. 1999. Lead intoxication: antioxidant defenses and oxidative damage in rat brain. Toxicology 135: 77–85. DOI: https://doi.org/10.1016/S0300-483X(99)00051-7

Bala S, Failla M L and Lunney J K. 1991. Alterations in splenic lymphoid cell subsets and activation antigens in copper- deficient rats. Journal of Nutrition 121: 745–53. DOI: https://doi.org/10.1093/jn/121.5.745

Bartoskewitz M L, Hewitt D G, Laurenz J C, Pitts J S and Bryant F C. 2007. Effect of dietary copper and zinc concentrations on white tailed deer antler growth, body size and immune system function. Small Ruminant Research 73: 87–94. DOI: https://doi.org/10.1016/j.smallrumres.2006.11.005

Bechara E J, Medeiros M H, Monteiro H P, Hermes-Lima M, Pereira B and Demasi M. 1993. A free radical hypothesis of lead poisoning and inborn porphyrias associated with 5– aminolevulinic acid overload. Química Nova 16: 385–92.

Burton J L, Kehrli M E, Jr, Kapil S and Horst R L. 1995. Regulation of L-selectin and CD18 on bovine neutrophils by glucocorticoids: effects of cortisol and dexamethasone. Journal of Leukocyte Biology 57: 317–25. DOI: https://doi.org/10.1002/jlb.57.2.317

Chen Q, Zhang M and Shen S. 2010. Effect of salt on malondialdehyde and antioxidant enzymes in seedling roots of Jerusalem artichoke (Helianthus tuberosus L.). Acta Physiologiae Plantarum 33(2): 273–78. DOI: https://doi.org/10.1007/s11738-010-0543-5

Colitis M, Stradioli G and Stefanon B. 2002. Regulation of mRNA expression of Phase I and Phase II enzymes in blood following administration of tomato and grape extract in sheep. Proceeding of the first European Symposium on Bioreactive Secondary Plants Products. Veterinary Medicine 32. Wien University of Veterinary Medicine.

Daniel L R, Chew B P, Tanaka T S and Tjoelker L W. 1991. In vitro effects of -carotene and vitamin A on paripartum bovine peripheral blood mononuclear cell proliferation. Journal of Dairy Science 74: 911–15. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78240-4

De Guise S, Bernier J and Dufresne M M. 1996. Immune functions in Beluga’ whales (Delphinapterus leucas): evaluation of mitogen-induced blastic transformation of lymphocytes from peripheral blood, spleen and thymus. Veterinary Immunology and Immunopathology 50: 117–26. DOI: https://doi.org/10.1016/0165-2427(95)05490-1

Deka R S, Mani V, Kumar M, Zade S S, Tyagi A K and Kaur H. 2014. Body condition, energy balance and immune status of periparturient murrah buffaloes (bubalus bubalis) supplemented with inorganic chromium. Biological Trace Element Research 161: 57–69. DOI: https://doi.org/10.1007/s12011-014-0069-6

Djoko Y, Cheryl-lynn Y, Ong Mark J, Walker and McEwan Alastair G. 2015. Copper and zinc toxicity and its role in innate immune defense against bacterial pathogens Karrera. Journal of Biological Chemistry 1–12. DOI: https://doi.org/10.1074/jbc.R115.647099

Ewers U, Stiller W R and Idel H. 1982. Serum immunoglobulin, complement C3 and salivary IgA levels in lead workers. Environmental Research 29: 352–57. DOI: https://doi.org/10.1016/0013-9351(82)90036-6

Flora S J S, Mittal M and Mehta A. 2008. Heavy metal induced oxidative stress and its possible reversal by chelation therapy. Indian Journal of Medical Research 128: 501–23.

Gabai G, Testoni S, Piccinini R, Marindli L and Stradaioli G. 2004. Oxidative stress in primiparous cows in relation to dietary starch and the progress of lactation. Animal Science 79: 99– 108. DOI: https://doi.org/10.1017/S1357729800054576

Hande G and Naran E. 2000. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radical Biology and Medicine 29: 927–45. DOI: https://doi.org/10.1016/S0891-5849(00)00413-5

Hsu J M. 1985. Lead toxicity related to glutathione metabolism. Journal of Nutrition 111: 26–33. DOI: https://doi.org/10.1093/jn/111.1.26

Ito Y, Niiya Y, Kurita H, Shima S and Sarai S. 1985. Serum lipid peroxide level and blood superoxide dismutase activity in workers with occupational exposure to lead. International Archives of Occupational and Environmental Health 56: 119– 27. DOI: https://doi.org/10.1007/BF00379383

Kang J K, Sul D, Kang J K, Nam S Y, Kim H J and Lee E. 2004. Effects of lead exposure on the expression of phospholipid hydroperoxidase glutathione peroxidase mRNA in the rat brain. Toxicological Sciences 82: 228–36. DOI: https://doi.org/10.1093/toxsci/kfh103

Kumar M, Kaur H, Phondba B T, Deka R S, Chandra G, Mani V and Gupta N. 2013. Effect of zinc treatments on lead exposed periparturient bovine lymphocytes in vitro on their proliferation and superoxide dismutase (SOD) expression. Indian Journal of Animal Sciences 83(12): 1261–66.

McGowan C and Donaldson W E. 1986. Changes in organ nonprotein sulfhydryl and glutathione concentrations during acute and chronic administration of inorganic lead to chicks. Biological Trace Element Research 10: 37–46. DOI: https://doi.org/10.1007/BF02795317

Mizuno Y. 1984. Superoxide dismutase activity in early stages of development in normal and dystrophic chickens. Life Science 34: 909–14. DOI: https://doi.org/10.1016/0024-3205(84)90294-7

Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assay. Journal of Immunological Methods 65: 55–63. DOI: https://doi.org/10.1016/0022-1759(83)90303-4

Mylroie A A, Collins H, Umbles C and Kyle J. 1986. Erythrocyte superoxide dismutase activity and other parameters of copper status in rats ingesting lead acetate. Toxicology and Applied Pharmacology 82: 512–20.

Mytroie AA. 1986. Erythrocyte SOD activity and other parameter of copper status in rats ingesting lead acetate. Toxicology and Applied Pharmacology 82: 512–20. DOI: https://doi.org/10.1016/0041-008X(86)90286-3

Nonnecke B J, Kimura K, Goff J P and Kehrli M E. 2003. Effect of the mammary gland on functional capacities of blood mononuclear leukocyte populations from periparturient cows. Journal of Dairy Science 86: 2359–68. DOI: https://doi.org/10.3168/jds.S0022-0302(03)73829-6

Noor R, Mittal S and Iqbal J. 2002. Superoxide dismutase- applications and relevance to human diseases. Medical Science Monitor 8: 210–15.

NRC. 2001. Nutrient Requirements of Dairy Cattle. 7th revised edn. National Academy Press, Washington, D.C.

Panda N, Kaur H and Mohanty T K. 2006. Reproductive performance of dairy buffaloes supplemented with varying levels of vitamin E. Asian Australasian Journal of Animal Sciences 19(1): 19–25. DOI: https://doi.org/10.5713/ajas.2006.19

Panemangalore M and Bebe F N. 1996. Effect of high dietary zinc on plasma cearuloplasmin and erythrocyte superoxide dismutase activities in copper-depleted and repleted rats. Biological Trace Element Research 55: 111–26. DOI: https://doi.org/10.1007/BF02784173

Poljsak B and Fink R. 2014. The protective role of antioxidants in the defence against ros/rns-mediated environmental pollution. Oxidative Medicine and Cellular Longevity 2014: 1–22. DOI: https://doi.org/10.1155/2014/671539

Rada B and Leto T L. 2008. Oxidative innate immune defenses by Nox/Duox family NADPH oxidases. Contributions to Microbiology 15: 164–87. DOI: https://doi.org/10.1159/000136357

Rajiv. 2001. ‘Influence of -carotene and vitamin E supplementation on udder health and immunocompetence in dairy cattle.’ PhD thesis submitted to National Dairy Research Institute (Deemed University), Karnal.

Sandhir R, Julka D and Gill K D. 1994. Lipoperoxidative damage on lead exposure in rat brain and its implications on membrane bound enzymes. Journal of Pharmacology and Toxicology 74: 66–71. DOI: https://doi.org/10.1111/j.1600-0773.1994.tb01077.x

Spears J W and Weiss W P. 2008. Role of antioxidants and trace elements in health and immunity of transition dairy cows. Veterinary Journal 176: 70–76. DOI: https://doi.org/10.1016/j.tvjl.2007.12.015

Sugawara E, Nakamura K, Miyake T, Fukumura A and Seki Y. 1991. Lipid peroxidation and concentration of glutathione in erythrocytes from workers exposed to lead. British Journal of Industrial Medicine 48: 239–42. DOI: https://doi.org/10.1136/oem.48.4.239

Weiss W P and Spears J W. 2006. Vitamin and trace mineral effects on immune function of ruminants. Ruminant Physiology. P. 473–496.Wageningen Academic Publishers, Utrecht, The Netherlands.

Wintergerst E S, Maggini S and Hornig D H. 2007. Contribution of selected vitamins and trace elements to immune function. Annals of Nutrition and Metabolism 51: 301–23. DOI: https://doi.org/10.1159/000107673