Polymorphism in the promoter region of HSP70 gene and its association with performance traits in Deoni cattle

K P RAMESHA; M BASAVARAJU; G R GEETHA; AKHILA RAO; D N HATKAR; M A KATAKTALWARE; K P SURESH; A ANANTHARAJ; D N DAS; P DIVYA

doi:10.56093/ijans.v86i12.66043

Authors

K P RAMESHA ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
M BASAVARAJU ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
G R GEETHA ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
AKHILA RAO ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
D N HATKAR ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
M A KATAKTALWARE ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
K P SURESH ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
A ANANTHARAJ ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
D N DAS ICAR-NDRI Southern Regional Station, Adugodi, Bengaluru, Karnataka 560 030 India
P DIVYA ICAR-NIVEDI, Bengaluru

https://doi.org/10.56093/ijans.v86i12.66043

Keywords:

Deoni cattle, HSP70, PCR-SSCP, Polymorphism, Reproductive traits

Abstract

A study was undertaken to analyze associations between HSP70 genetic variants of promoter region with heat tolerance and performance in Deoni (Bos indicus) cattle using PCR-SSCP technique. The analysis revealed three SSCP patterns, viz. AA, AB and BB with frequencies of 0.712, 0.254 and 0.034, respectively. Sequence analysis of representative samples of each pattern showed 7 SNPs, which included four transitions (A859G, A1046G, C1257T and G1294A) and 3 transversions (A897C, A1127C and G1166T). Cows with different SSCP patterns showed similar production and reproduction performances. The studies on physiological parameters and calculation of Heat tolerance coefficient (HTC) of individual cows indicated that Deoni cows maintained at Bengaluru were not under heat stress even after 6 h of exposure to sun during summer (March to May).

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