Seroepidemiology of paratuberculosis in cattle population of organized and unorganized farms of India

SHIRISH DADARAO NARNAWARE; BHUPENDRA NATH TRIPATHI

doi:10.56093/ijans.v87i1.66797

Authors

SHIRISH DADARAO NARNAWARE ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122 India
BHUPENDRA NATH TRIPATHI ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v87i1.66797

Keywords:

Cattle, ELISA, Paratuberculosis, Seroprevalence

Abstract

Serum samples (531) collected randomly from cattle of organized (200) and unorganized (331) farms of Central and Northern parts of India were subjected to a commercial ELISA to know the seroprevalence of paratuberculosis. These serum samples were also tested by an in-house absorbed ELISA developed in the laboratory and the results were compared. The overall seroprevalence of paratuberculosis in cattle was 8.09% with significantly higher prevalence in cattle of organized farms (13.5%) than unorganized farms (4.83%). The seroprevalence was also significantly higher in calves (17.24%) than adults (7.57%); whereas there was no significant difference in prevalence rate among male (6.71%) and female (9.87%) cattle. Region wise the seroprevalence was slightly higher in organized farms of Northern India (16.43%) than that of Central India (11.81%). The sensitivity and specificity of in-house ELISA were 71.11% and 98.76%, respectively, with the accuracy of over 96%. On the basis of Kappa-test, the inhouse ELISA was in good agreement with commercial Pourquier ® ELISA and can be recommended for screening of paratuberculosis infection in cattle in India.

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