A TaqMan real time probe assay for specific detection and quantification of lapinized vaccine strain of classical swine fever virus
247 / 97
Authors
-
JENY K JOHN
ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
-
R ANOOPRAJ
ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
-
NEELAM TOMAR
ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
-
G SAIKUMAR
ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
Keywords:
CSFV, Quantification, Real-time, Standard curve, TaqManAbstract
Classical swine fever (CSF) is a highly contagious viral disease having a tremendous socio-economic impact. In CSF endemic countries such as India, control strategies depend heavily on the use of a live attenuated vaccine. The persistence of vaccine virus in tissue/blood of vaccinated animals may interfere with the diagnosis when RT-PCR assay is used for CSF diagnosis. A TaqMan based real-time RT-PCR assay was developed with primers and probe targeting the NS5B region of the lapinized CSFV (vaccine strain) used in India for detection and quantification of lapinized CSFV. The real-time TaqMan RT-PCR assay presented in the current study provided a rapid, specific and sensitive diagnostic tool for the detection of LCSFV and for quantification of virus load in various organs. This real time RT-PCR assay can differentiate the infected from vaccinated animals and quantification assay can be used for post-vaccination studies.
Downloads
References
Liu J, Fan X, Wang Q, Xu L, Zhao Q, Huang W, Zhou Y, Tang B, Chen L, Zou X, Sha S and Zhu Y. 2011. Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs. Virology Journal 8: 201. DOI: https://doi.org/10.1186/1743-422X-8-201
Ophuis R J, Morrissy C J and Boyle D B. 2006. Detection and quantitative pathogenesis study of classical swine fever virus using a real time RT-PCR assay. Journal of Virological Methods 131: 78-85. DOI: https://doi.org/10.1016/j.jviromet.2005.07.008
Risatti G R, Callahan J D, Nelson W M and Borca M V. 2003. Rapid detection of classical swine fever virus by a portable real-time reverse transcriptase PCR assay. Journal of Clinical Microbiology 41: 500–05. DOI: https://doi.org/10.1128/JCM.41.1.500-505.2003
Uttenthal A, Storgaard T, Oleksiewicz M B and de Stricker K. 2003. Experimental infection with the Paderborn isolate of classical swine fever virus in 10–week old pigs:determination of viral replication kinetics by quantitative RT-PCR, virus isolation and antigen ELISA. Veterinary Microbiology 4: 117–32. DOI: https://doi.org/10.1016/S0378-1135(02)00364-4
Zhao J J, Cheng D, Li N, Sun Y, Shi Z, Zhu Q H, Tu C, Tong G Z and Qiu H J. 2008. Evaluation of a multiplex real-time RT- PCR for quantitative and differential detection of wild-type viruses and C strain vaccine of Classical swine fever virus. Veterinary Microbiology 126: 1–10. DOI: https://doi.org/10.1016/j.vetmic.2007.04.046
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2017 The Indian Journal of Animal Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.
How to Cite
