Genetic diversity analysis of Chaugarkha goat using microsatellite markers
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Authors
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Z A GANIE
Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
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D KUMAR
Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
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SUNDIP KUMAR
Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
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B N SHAHI
Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
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A K GHOSH
Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
Keywords:
Chaugarkha goat, Diversity analysis, Molecular characterization, PolymorphismAbstract
Genetic variability within Chaugarkha breed of goat was studied using microsatellite markers from 25 blood samples of unrelated animals. A set of 15 microsatellite markers were used for PCR and the DNA profiles were scored from the gel that were further analyzed using standard population analysis software. The observed number of alleles varied from 5 to 9 with a mean of 7.20±0.34. The effective number of alleles varied from 2.62 to 6.76 with a mean of 4.82±0.07. The average observed heterozygosity ranged from 0.80 to 0.92 with an average value of 0.83±0.01. The average expected heterozygosity ranged from 0.63 to 0.87 with an average value of 0.79±0.02. The within population Nei’s average expected heterozygosity/gene diversity ranged from 0.24 to 0.85 with an average value of 0.32±0.02. The PIC values ranged from 0.24 to 0.54 with an average of 0.32±0.02. The values of Shannon’s information index were in the range of 1.14 to 2.00 with an average of 1.67±0.07. The level of genetic variability indicated the possibility for further genetic improvement of Chaugarkha goats.
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References
Botstein D, White R L, Skolnick M and Davis R W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32: 314–31.
Deshmukh B, Kumar D, Kashyap N and Sharma D. 2015. Study of genetic polymorphism of various chicken breeds using microsatellite markers. Indian Journal of Animal Research 49(1): 1–7. DOI: https://doi.org/10.5958/0976-0555.2015.00001.1
Dixit S P, Aggarwal R A K, Verma N K, Viyas K, Rana J, Sharma A and Chander R. 2011. Genetic variability and bottleneck analyses of Kanniadu goat breed based on microsatellite markers. Indian Journal of Animal Sciences 81: 40–43.
Fatima S. 2006. ‘Study of Genetic variability among Gohilwadi, Surti and Zalawadi goats using microsatellite analysis.’ M.V.Sc. thesis submitted to Anand Agricultural University, Anand, Gujarat, India.
Kumar D and Gaur A K. 2015. Performance studies of hill cattle: A unique indigenous germplasm of Uttarakhand. Indian Journal of Animal Sciences 85(12): 1390–92.
Kumar D and Gaur A K. 2016. Studies on hill cattle of Pithoragarh district in Uttarakhand. International Journal of Research and Scientific Innovation 2(3): 44–52.
MacHugh D E, Shriver M D, Loftus R T, Cunningham P and Bradley D G. 1997. Microsatellite DNA variation and the evolution, domestication and phylogeography of taurine and zebu cattle (Bos taurus and Bos indicus). Genetics 146: 1071–86. DOI: https://doi.org/10.1093/genetics/146.3.1071
Nei M. 1987. Molecular Evolutionary Genetics. Columbia University Press, New York, NY, USA. DOI: https://doi.org/10.7312/nei-92038
Pandey A K, Sharma Rekha, Singh L V, Maitra A, Mishra B P and Kumar D. Estimation of genetic variability parameters in Kumaun hill cattle (Kumauni cattle) by STR markers. Indian Journal of Animal Sciences 81(2): 194–95.
Peakall R and Smouse P E. 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. DOI: https://doi.org/10.1093/bioinformatics/bts460
Sambrook J and Russell D W. 2001. Molecular Cloning: A Laboratory Manual. 3rd edn. Cold Spring Harbor Laboratory Press, New York.
Shannon C E and Weaver W. 1949. The Mathematical Theory of Communication. University of Illinois Press, IL.
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