Estimating serological immune response against Newcastle disease vaccine in Aseel, Kadaknath and White Leghorn chicken by haemagglutination inhibition test

LAXMIKANT SAMBHAJI KOKATE; SANJEEV KUMAR; ABDUL RAHIM; ANANTA KUMAR DAS

doi:10.56093/ijans.v87i2.67681

Authors

LAXMIKANT SAMBHAJI KOKATE ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
SANJEEV KUMAR ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ABDUL RAHIM ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ANANTA KUMAR DAS ICAR-Central Avian Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v87i2.67681

Keywords:

Aseel, HI antibody titre, Kadaknath, NDV vaccine response, White Leghorn chicken

Abstract

The present study aimed to estimate serological immune response against Newcastle disease vaccine investigating
70 Aseel, 75 Kadaknath and 85 White Leghorn chicks. The day-old chicks were vaccinated with a dose of 106.5
EID50 of RDF1 strain through occulo-nasal route followed by a booster dose on 28th day. The sera collected on 7,
14, 21, 28, 35 and 42 days post-immunization (dpi) were used to measure antibody titres through haemagglutination
inhibition test. The data were analyzed by analysis of variance using SPSS 16.0 statistical software. The corresponding mean antibody titre (log2) estimates were 8.10±0.22, 7.89±0.18, 7.92±0.16, 8.29±0.14, 8.40±0.16 and 8.94±0.19 in Aseel, 7.32±0.16, 7.74±0.13, 7.56±0.13, 7.86±0.17, 8.43±0.17 and 9.14±0.16 in Kadaknath, and 8.48±0.28,
8.02±0.31, 8.29±0.33, 8.14±0.30, 7.68±0.29 and 7.73±0.29 in White Leghorn chicken. The estimates significantly
varied among different dpi in Aseel and Kadaknath chicken except White Leghorn. Aseel and Kadaknath chicken
demonstrated gradual increasing trend and higher means of antibody titres for longer periods of dpi and achieved
the highest at 42 dpi, whereas White Leghorn chicken showed an irregular trend, the highest titre being observed at
7 dpi. Again the 3 chicken genotypes significantly varied in antibody titres at 7, 35 and 42 dpi; White Leghorn
chicken demonstrated the highest antibody titre at 7 dpi, while Kadaknath chicken showed the highest titre at 35
and 42 dpi. The higher and longer immune responsive Aseel and Kadaknath chicken might be utilized for selective
introgression of their candidate genes in high productive chicken germplasm with less NDV response.

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