Studies on expression of different virulence genes of Pasteurella multocida

SIVARAMAKRISHNA AKONDI; A K ARORA; N S SHARMA

doi:10.56093/ijans.v87i2.67687

Authors

SIVARAMAKRISHNA AKONDI Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
A K ARORA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India
N S SHARMA Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 India

https://doi.org/10.56093/ijans.v87i2.67687

Keywords:

Iron limitation, Pasteurella multocida, Real Time PCR, Virulence gene expression

Abstract

Pasteurella multocida is the causative agent of a wide range of diseases in avian and mammalian hosts. Different adhesin and membrane proteins play role in the pathogenesis of the disease. In the present study, the relative expression of 5 different virulence genes (plpE, ptfA, tbpA, hgbA and fhaB1) from Pasteurella multocida B:2 grown in iron rich and iron limiting media was measured using real time PCR employing SYBR green chemistry. The expression of tbpA, hgbA, plpE and fhaB1 was found to be significantly upregulated by 4, 2.3, 1.3, 2.3 folds, respectively, under iron limiting conditions. In contrast, the expression of gene ptfA was significantly down regulated (0.4 fold) as compared to organism grown in normal medium.

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