Influence of exogenous supplementation of IGF-I, cysteamine and their combination on in vitro caprine blastocyst development

PUJA GOEL; A K GOEL; A K BHATIA; S D KHARCHE

doi:10.56093/ijans.v87i2.67707

Authors

PUJA GOEL ICAR-Central Institute for Research on Goats, Mathura, Uttar Pradesh 281 122 India
A K GOEL ICAR-Central Institute for Research on Goats, Mathura, Uttar Pradesh 281 122 India
A K BHATIA ICAR-Central Institute for Research on Goats, Mathura, Uttar Pradesh 281 122 India
S D KHARCHE ICAR-Central Institute for Research on Goats, Mathura, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v87i2.67707

Keywords:

Blastocyst, Caprine, Cleavage, Cysteamine, IGF-I, IVF, Morula, Oocyte

Abstract

The present study was carried out to investigate the putative beneficial effects of insulin-like growth factor-I (IGF-I) and cysteamine supplementation alone or their combination on in vitro embryo development competence of fertilized goat oocytes. Presumptive zygotes (18 h post insemination) were randomly assigned for in vitro embryo development in embryo development medium (EDM) supplemented with IGF-I (Gr. 1), Cysteamine (Gr. 2), IGF- I + Cysteamine (Gr. 3) and Control containing only EDM (Gr. 4). Statistically non-significant difference was observed in cleavage rate among all the treated groups. Morula formation rate was significantly higher in IGF-I supplemented group compared to IGF-I + cysteamine supplemented and non-supplemented (control) groups. Furthermore, supplementation of IGF-I, cysteamine and IGF-I + cysteamine in embryo culture medium significantly improved blastocyst formation rate compared to control. However, a nonsignificant difference in blastocyst formation was observed among the supplemented groups. These findings lead to the conclusion that under in vitro conditions, supplementation of IGF-I and cysteamine alone or combination in IVC media were equally effective in embryo development and blastocyst production, however, this effect was significantly higher as compared to non- supplemented group (control).

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References

De A K, Malakar D, Akshey Y S, Jena M K, Garg S, Dutta R and Sahu S. 2011. In vitro development of goat (Capra hircus) embryos following cysteamine supplementation of the in vitro maturation and in vitro culture media. Small Ruminant Research 96: 185–90. DOI: https://doi.org/10.1016/j.smallrumres.2011.01.001

de Matos D G, Gasparrini B, Pasqualini S R and Thompson J G. 2002. Effect of glutathione synthesis stimulation during in vitro maturation of ovine oocytes on embryo development and intracellular peroxide content. Theriogenology 57: 1443–51. DOI: https://doi.org/10.1016/S0093-691X(02)00643-X

Favetta L A, Robert C, King W A and Betts D H. 2004a. Expression profiles of p53 and p66shc during oxidative stress- induced senescence in fetal bovine fibroblasts. Experimental Cell Research 299: 36–48. DOI: https://doi.org/10.1016/j.yexcr.2004.05.009

Favetta L A, Robert C, St John E J, Betts D H and King W A. 2004b. p66shc, but not p53, is involved in early arrest of in vitro-produced bovine embryos. Molecular Human Reproduction 10: 383–92. DOI: https://doi.org/10.1093/molehr/gah057

Goncalves F S, Barretto L S S, Perri S H V and Mingoti G Z. 2006. Bovine in vitro fertilization with cysteamine, 2-β mercaptoethanol and heparin. Acta Scientiae Veterinariae 34: S- 453.

Hao Y, Lai L, Mao J, Im G S, Bonk A and Prather R S. 2003. Apoptosis and in vitro development of preimplantation porcine embryos derived in vitro or by nuclear transfer. Biology of Reproduction 69: 501–07. DOI: https://doi.org/10.1095/biolreprod.103.016170

Kadakia R, Arraztoa J A, Bondy C and Zhou J. 2001. Granulosa cell proliferation is impaired in the Igf 1 null ovary. Growth Hormone IGF Research 11: 220–24. DOI: https://doi.org/10.1054/ghir.2001.0201

Kharche S D, Goel A K, Jindal S K, Goel P and Jha B K. 2011. Birth of twin kids following transfer of in-vitro produced goat embryos. Indian Journal of Animal Sciences 81: 1132–34.

Kharche S D, Goel A K, Jindal S K, Sinha N K and Yadav P. 2008. Effect of somatic cells co-culture on cleavage and development of in-vitro fertilized caprine embryos. Indian Journal of Animal Sciences 78: 686–92.

Kharche S D, Agrawal S, Pathak J, Sikarwar A K S, Gangawar Chetna, Ranjan Ravi, Goel A K, Jindal S K and Agarwal S K. 2016. Influence of cysteamine supplementation during in vitro culture of early stage caprine embryos on blastocyst production. Indian Journal of Animal Sciences 86(3): 304–06.

Kitagawa Y, Suzuki K, Yonder A and Watanabe T. 2004. Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS) and DNA fragmentation in porcine embryos. Theriogenology 62: 1186–97. DOI: https://doi.org/10.1016/j.theriogenology.2004.01.011

Lima P F, Oliveira M A L, Santos M H B, Reichenbach H D, Weppert M, Paula-Lopes F F, Cavalcanti Neto C C and Goncalves P B D. 2006. Effect of retinoids and growth factor on in vitro bovine embryos produced under chemically defined conditions. Animal Reproduction Science 95:184–92. DOI: https://doi.org/10.1016/j.anireprosci.2005.08.013

Pushpakumara P G A, Robinson R S, Demmers K J, Mann G E, Sinclair K D, Webb R et al. 2002. Expression of the insulin like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy. Reproduction 123: 859–68. DOI: https://doi.org/10.1530/rep.0.1230859

Robinson R S, Mann G E, Gadd T S, Lamming G E and Wathes D C. 2000. The expression of the IGF system in the bovine uterus throughout the oestrous cycle and early pregnancy. Journal of Endocrinology 165: 231–43. DOI: https://doi.org/10.1677/joe.0.1650231

Shabankareh H K and Zandi M. 2010. Developmental potential of sheep oocytes cultured in different maturation media: effects of epidermal growth factor, insulin-like growth factor I and cysteamine. Fertility and Sterility 94: 335–40. DOI: https://doi.org/10.1016/j.fertnstert.2009.01.160

Sirisathien S, Hernandez-Fonseca H J and Brackett B G. 2003. Influences of epidermal growth factor and insulin-like growth factor-I on bovine blastocyst development in vitro. Animal Reproduction Science 77: 21–32. DOI: https://doi.org/10.1016/S0378-4320(02)00272-5

Snedecor G W and Cochran W G. 1989. Statistical Methods. 6th edn. The lowa State University Press, USA.

Stefanello J R, Barreta M H, Porciuncula P M, Arruda J N, Oliveira J F, Oliveira M A et al. 2006. Effect of angiotensin II with follicle cells and insulin-like growth factor-I or insulin on bovine oocyte maturation and embryo development. Theriogenology 66: 2068–76. DOI: https://doi.org/10.1016/j.theriogenology.2006.06.005

Takahashi M, Nagai T, Okamura N, Takahashi H and Okano A. 2002. Promoting effect of beta-mercaptoethanol on in vitro development under oxidative stress and cystine uptake of bovine embryos. Biology of Reproduction 66: 562–67. DOI: https://doi.org/10.1095/biolreprod66.3.562

Tatemoto H, Ootaki K, Shigeta K and Muto N. 2001. Enhancement of developmental competence after in vitro fertilization of porcine oocytes by treatment with ascorbic acid 2–O-a- glucoside during in vitro maturation. Biology of Reproduction 65: 1800–06. DOI: https://doi.org/10.1095/biolreprod65.6.1800

Velazquez M A, Spicer L J and Wathes D C. 2008. The role of endocrine insulin-like growth factor-I (IGF-I) in female bovine reproduction. Domestic Animal Endocrinology 35: 325–42. DOI: https://doi.org/10.1016/j.domaniend.2008.07.002

Winger Q A, De los Rios P, Han V K, Armstrong D T, Hill D J and Watson A J. 1997. Bovine oviductal and embryonic insulin- like growth factor binding proteins: possible regulators of embryotrophic insulin-like growth factor circuits. Biology of Reproduction 56: 1415–23. DOI: https://doi.org/10.1095/biolreprod56.6.1415

Yaseen M A, Wrenzycki C, Herrmann D, Carnwath J W and Niemann H. 2001. Changes in the relative abundance of mRNA transcripts for insulin-like growth factor (IGF-I and IGF-II) ligands and their receptors (IGF-IR/IGF-IIR) in preimplantation bovine embryos derived from different in vitro systems. Reproduction 122: 601–10. DOI: https://doi.org/10.1530/rep.0.1220601