Cytotoxic potential of rhizome extracts of Hedychium spicatum L. in HepG2 cell line using MTT


Abstract views: 53 / PDF downloads: 76

Authors

  • GOVIND KUMAR CHOUDHARY G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India
  • SATYA PAL SINGH G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145 India

https://doi.org/10.56093/ijans.v87i3.68855

Keywords:

Cytotoxicity, Hedychium spicatum, HepG2, MTT

Abstract

The present study was designed to evaluate the cytotoxic effects of extract of Hedychium spicatum rhizome extract on human liver hepatocellular carcinoma (HepG2) cell line using MTT assay. The crude extract of H. spicatum was prepared by cold maceration method, filtered, concentrated in different organic solution and tested for phytochemical evaluation and finally on HepG2 cell line. Dose-dependent cytotoxic activities were exhibited on HepG2 cell line. As the dose of the extract increased, the number of viable cells decreased. This confirms the cytotoxic potential of the rhizome of H. spicatum. The IC50 value of different extracts was determined and concluded that the methanolic extract had better potential than the other extracts.

Downloads

Download data is not yet available.

References

Alison M R. 2005. Liver stem cells: implications for hepatocarcinogenesis. Stem Cell Reviews 1(3): 253–60. DOI: https://doi.org/10.1385/SCR:1:3:253

Busuttil R W and Farmer D G. 1996. The surgical treatment of primary hepatobiliary malignancy. Liver Transpl Surgery 2(5 Suppl 1):114–30.

Edeoga H O, Okwu D E and Mbaebie B O. 2005. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology 4: 685–88. DOI: https://doi.org/10.5897/AJB2005.000-3127

Fatope M O, Ibrahim H and Takeda Y. 1993. Screening of higher plants reputed as pesticides using the brine shrimp lethality assay. International Journal of Pharmacognosy 31: 250 –54. DOI: https://doi.org/10.3109/13880209309082949

Penn I. 1991. Hepatic transplantation for primary and metastatic cancers of the liver. Surgery 110(4): 726–34.

Reddy P P, Lavekar A G, Suresh Babu K, Rao R R, Shashidhar J and Rao M. 2010. Synthesis, cytotoxic activity and structure– activity relationships of hedychenone analogues. Bioorganic and Medicinal Chemistry Letters 20: 2525–28. DOI: https://doi.org/10.1016/j.bmcl.2010.02.101

Sofowora A. 1984. Medicinal Plants and Traditional Medicine in Africa. John Wiley and Sons, pp 128–70.

Suresh G, Poornima B, Suresh Babu K, Yadav P A, Rao M S A, Rajendra Prasad K, Nayak V L and Ramakrishna S. 2013. Cytotoxic sesquiterpenes from Hedychium spicatum: Isolation, structure elucidation and structure–activity relationship studies. Fitoterapia 86: 100–07. DOI: https://doi.org/10.1016/j.fitote.2013.02.004

Downloads

Submitted

2017-03-20

Published

2017-03-21

Issue

Section

Short-Communication

How to Cite

CHOUDHARY, G. K., & SINGH, S. P. (2017). Cytotoxic potential of rhizome extracts of Hedychium spicatum L. in HepG2 cell line using MTT. The Indian Journal of Animal Sciences, 87(3), 313–315. https://doi.org/10.56093/ijans.v87i3.68855
Citation