Effect of membrane stabilizer on the freezability of buck semen

R RANJAN; R PRIYADHARSINI; A K GOEL; BREJENDRA SINGH; SATISH KUMAR; S D KHARCHE; S K JINDAL

doi:10.56093/ijans.v87i4.69516

Authors

R RANJAN ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
R PRIYADHARSINI ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
A K GOEL ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
BREJENDRA SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
SATISH KUMAR ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
S D KHARCHE ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
S K JINDAL ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v87i4.69516

Keywords:

Acrosome, Buck semen, Chlorpromazine hydrochloride, Cryopreservation, HOS

Abstract

Chlorpromazine hydrochloride (CPH) acts as a membrane stabilizer during semen freezing. The ejaculates from Jamunapari bucks (2–4 years old) maintained at this institute under semi intensive management system were utilized to find out the freezability of buck semen at different levels of CPH by conventional method of freezing. The ejaculates were collected twice a week using artificial vagina and were extended to maintain sperm concentration approximately 100 million per dose (0.25 ml) with Tris- Citric acid- Fructose (TCF) diluent. The post thaw motility, live sperm count, abnormalities, acrosomal integrity and hypo-osmotic swelling positive spermatozoa did not differ significantly at different levels of CPH. There was no significant effect of CPH inclusion in goat semen dilutor on post thaw qualities. None of the levels of CPH tested improved sperm survival, and the highest level of drug (400 µM) was found to be spermicidal.

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