Effect of zinc oxide nanoparticles on some antioxidant biomarkers and enzymes of broiler chickens during starter phase

F AHMADI; Y EBRAHIMNEZHAD; N MAHERI-SIS; J GHIASI GHALEH-KANDI

doi:10.56093/ijans.v87i4.69604

Authors

F AHMADI Islamic Azad University, Shabestar, Iran
Y EBRAHIMNEZHAD Islamic Azad University, Shabestar, Iran
N MAHERI-SIS Islamic Azad University, Shabestar, Iran
J GHIASI GHALEH-KANDI Islamic Azad University, Shabestar, Iran

https://doi.org/10.56093/ijans.v87i4.69604

Keywords:

Antioxidative, Blood, Broiler, Enzyme, Nanoparticles

Abstract

The study investigated the effects of zinc oxide nanoparticles on some of the antioxidant biomarkers and enzyme serum in broiler chickens during starter stage (1-21d). A total of 240 one-d-old broilers (Ross-308) were randomly divided into four groups, with 60 birds in each group and 4 replicates with 15 birds in each experimental pen. The experimental diets were T1, Control (basal corn-soybean diet supplemented with 36.27 mg Zn/kg from zinc oxide; T2, T3 and T4, basal corn-soybean diets supplemented with 30, 60 and 90 mg ZnO-NPs/kg, respectively. Dietary ZnO-NPs supplementation significantly increased total antioxidant capacity (TAC), super oxide dismutase (SOD) and glutathione peroxidase (GSH-Px) enzyme activity in test groups compared to the control. However, the serum enzyme activity of lactate dehydrogenase, alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase in the birds fed ZnO-NPs had not significantly altered in comparison to control group. However, a decreasing trend was observed in the activity of ALT and AST enzymes in the birds fed 60 and/or 90 mg ZnONPs. Also, the MDA concentration was numerically decreased compared to the control group. In conclusion, results of study suggest that dietary zinc oxide nanoparticles @ 90 mg per kg of basal diet improved antioxidative status and activity of some enzymes used as body health indicator in the broilers during starter period (1-21 d).

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