Tetra-primer ARMS-PCR assay for genotyping SNPs in ovine GDF8 gene associated with mutton traits in sheep

REENA ARORA; D K YADAV; ANJU SHARMA; MOUNIKA POTHURAJU; NEETIKA TANWAR; YASHILA GIRDHAR

doi:10.56093/ijans.v87i4.69619

Authors

REENA ARORA ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
D K YADAV ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
ANJU SHARMA ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
MOUNIKA POTHURAJU ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
NEETIKA TANWAR ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India
YASHILA GIRDHAR ICAR-National Bureau of Animal Genetic Resources, Karnal, Haryana 132 001 India

https://doi.org/10.56093/ijans.v87i4.69619

Keywords:

Association, GDF8, Indian sheep, T-ARMS-PCR

Abstract

Our study indicates the effects of genotypes on paunch girth. The results need to be verified on a larger number of samples. Nonetheless, our study emphasizes the efficacy of the T-ARMS PCR assay for increasing throughput. These assays will enable rapid and cost effective screening of large number of samples for association studies. The generated information can then be utilized in formulation of breeding policies for Indian sheep.

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