Comparative evaluation of FAT, IS900 PCR and microscopy vis-a-vis histopathology for the detection of Mycobacterium avium subsp paratuberculosis infection in tissues of goats naturally died in herds endemic for Johne’s disease

SHIVAM CHATURVEDI; SHOOR VIR SINGH; ASHOK KUMAR SRIVASTAVA; NEERAJ KUMAR GANGWAR; NAVEEN KUMAR; KRISHNA DUTTA RAWAT; SAURABH GUPTA; KUNDAN KUMAR CHAUBEY; RAJENDRA SINGH; RAGHAVENDRA SINGH; KULDEEP DHAMA

doi:10.56093/ijans.v87i6.71061

Authors

SHIVAM CHATURVEDI ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
SHOOR VIR SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
ASHOK KUMAR SRIVASTAVA ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
NEERAJ KUMAR GANGWAR ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
NAVEEN KUMAR ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
KRISHNA DUTTA RAWAT ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
SAURABH GUPTA ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
KUNDAN KUMAR CHAUBEY ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
RAJENDRA SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
RAGHAVENDRA SINGH ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India
KULDEEP DHAMA ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh 281 122 India

https://doi.org/10.56093/ijans.v87i6.71061

Keywords:

Diagnosis, Goats, Multiple tests, Mycobacterium avium subspecies paratuberculosis

Abstract

Study investigated infection of Mycobacterium avium subspecies paratuberculosis (MAP) in paired tissues viz. ileum and mesenteric lymph nodes (MLN) from 74 goats using histopathology, acid fast staining of tissue sections, fluorescent antibody test (FAT), IS900 tissue PCR and culture. Histopathology of H&E stained tissue sections revealed variable grades of lesions of Johne's disease (JD) in 37 (50.0%) ileae and 21 (28.4%) MLN. In AFB staining, 16 (21.6%) intestine and 10 (13.5%) MLN revealed presence of acid fast bacilli indistinguishable to MAP. Thirteen (17.5%) intestine and 9 (12.1%) MLN were positive in FAT. Eleven (14.8%) intestine and 7 (9.4%) MLN were positive by IS900 tissue PCR, whereas 7 (9.5%) intestine and 8 (10.8%) MLN were positive in culture. The study showed that sensitivity of histopathology (H&E staining) was highest as compared to other routine tests for the diagnosis of MAP infection in tissues of goats died (irrespective of cause of deaths) during study period from goat herds endemic for Johne's disease. Lower sensitivity of FAT than histology was due to the strong cell mediated immunity exhibited by MAP. Though difference was not significant between sensitivities of FAT and PCR, FAT and AFB staining, and agreement was very good; however, results of the study suggested, that FAT may be a useful test to improve the diagnostic efficacy of caprine Johne's disease and more effective in the detection of grade I lesions as compared to culture and PCR. Study also showed that it was essential to collect both tissues (intestines and MLNs) for the diagnosis of MAP infection at necropsy.

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