Antioxidant potential of coenzyme Q10 in Escherichia coli associated calf diarrhea

J GARKHAL; G E CHETHAN; V K GUPTA; S QURESHI; R MUKHERJEE; U DIMRI; G K GAUR; R K AGARWAL; U K DE

doi:10.56093/ijans.v87i6.71063

Authors

J GARKHAL Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
G E CHETHAN Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
V K GUPTA Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
S QURESHI Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
R MUKHERJEE Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
U DIMRI Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
G K GAUR Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
R K AGARWAL Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India
U K DE Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 241 322 India

https://doi.org/10.56093/ijans.v87i6.71063

Keywords:

Calf diarrhea, Coenzyme Q10, Escherichia coli, IFABP 2, Oxidative stress

Abstract

The aim of the present study was to evaluate the antioxidant potential of coenzyme Q10 in Escherichia coli associated calf diarrhea. Six diarrheic calves were treated with standard treatment (ST) and another six diarrheic calves were given standard treatment along with coenzyme Q10 (ST-CoQ10). Whereas, six healthy calves received only placebo. The results revealed a significant reduction of Hb, PCV, TLC, albumin, BUN, creatinine, oxidative stress indicators, IFABP2, fecal consistency score and dehydration (%) in diarrheic calves treated with either ST or ST-CoQ10 on day 3 and 5. However, ST-CoQ10 treatment effectively reduced the oxidative stress indicators and IFABP 2 much earlier (day 3) compared to ST treatment alone. The results of the present study suggest that the incorporation of CoQ10 in standard treatment represents a potential additional treatment option in the case of E. coli associated calf diarrhea.

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References

Adarsh K, Kaur H and Mohan V. 2008. Coenzyme Q10 (CoQ10) in isolated diastolic heart failure in hypertrophic cardiomyopathy (HCM). Biofactors 32: 145–49. DOI: https://doi.org/10.1002/biof.5520320117

Ahmed W M and Hassan S E. 2007. Applied studies on coccidiosis in growing Buffalo calves with special reference to oxidant/ antioxidant status. World Journal of Zoology 2: 40–48.

Bandyopadhyay S, Patra P, D Mondal K, Biswas T, Batabyal S and Lodh C. 2015. Erythrocytic oxidative stress indices, biochemical and trace mineral milieu in yaks with persistent haemorrhagic diarrhoea associated with enterovirulent Escherichia coli. Indian Journal of Animal Sciences 85: 604– 06.

Bartels C J, Holzhauer M, Jorritsma R, Swart W A and Lam T J. 2010. Prevalence, prediction and risk factors of enteropathogens in normal and non-normal faeces of young Dutch dairy calves. Preventive Veterinary Medicine 93: 162– 69. DOI: https://doi.org/10.1016/j.prevetmed.2009.09.020

Bellamy J E and Acres S D. 1979. Enterotoxigenic colibacillosis in colostrum-fed calves: pathologic changes. American Journal of Veterinary Research 40(10): 1391–97.

Blom J Y. 1981. The relationship between serum immunoglobulin values and incidence of respiratory disease and enteritis in calves. Nordisck Vetrinaermedicin 34: 276–84.

Cadenas E, Hochstein P P and Ernster L. 1992. Pro- and antioxidant functions of quinones and quinone reductases in mammalian cells. Advances in Enzymology and Related Areas of Molecular Biology 65: 97–147. DOI: https://doi.org/10.1002/9780470123119.ch3

Cho Y and Yoon K. 2014. An overview of calf diarrhea - infectious etiology, diagnosis, and intervention. Journal of Veterinary Science 15: 1–17. DOI: https://doi.org/10.4142/jvs.2014.15.1.1

Clement J C, King M E, Salman M D, Wittum T E, Casper H H and Odde K G. 1995. Use of epidemiologic principles to identify risk factors associated with the development of diarrhoea in calves in five beef herds. Journal of the American Veterinary Medical Association 207: 1334–38.

Constable P D, Walker P G and Morin D E. 1998. Clinical and laboratory assessment of hydration status in neonatal calves with diarrhoea. Journal of the American Veterinary Medical Association 212: 991–96.

Cummins K A and Brunner C J. 1989. Dietary ascorbic acid and immune response in dairy calves. Journal of Dairy Science 72: 129–34. DOI: https://doi.org/10.3168/jds.S0022-0302(89)79088-3

De U K, Mukherjee R, Nandi S, Hanumatnagouda B, Patel B H M, Dimri U, Ravishankar C and Verma A K. 2014. Alterations in oxidant/antioxidant balance, high mobility group box 1 protein and acute phase response in cross-bred suckling piglets suffering from rotaviral enteritis. Tropical Animal Health and Production 46: 1127–33. DOI: https://doi.org/10.1007/s11250-014-0616-3

Drapalo A W, Jaroszewicz J, Siwak E, Pogorzelska J and Prokopowicz D. 2008. Intestinal fatty acid binding protein (I- FABP) as a possible biomarker of ileitis in patients with ulcerative colitis. Regulatory Peptides 147: 25–28. DOI: https://doi.org/10.1016/j.regpep.2007.12.002

Draper H H and Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation. Methods Enzymology 186: 421– 31. DOI: https://doi.org/10.1016/0076-6879(90)86135-I

Edelson M B, Sonnino R E, Bagwell C E, Lieberman J M, Marks W H and Rozycki H J. 1993. Plasma intestinal fatty acid binding protein in neonates with necrotizing enterocolitis: a pilot study. Journal of Pediatric Surgery 28: 367–70.

El-Halfawy O M and Valvanoa M A. 2014. Putrescine reduces antibiotic-induced oxidative stress as a mechanism of modulation of antibiotic resistance in Burkholderia Cenocepacia. Antimicrobial Agents and Chemotherapy 58: 4162–71. DOI: https://doi.org/10.1128/AAC.02649-14

Frankel S S, Reitman S and Sonnenwirth A C. 1970. Grandwoh’s Clinical Laboratory Methods and Diagnosis. p 325–35. The C. V. Mosby Company, St Louis, USA.

Funaoka H, Kanda and T Fujii. 2010. Intestinal fatty acid-binding protein (I-FABP) as a new biomarker for intestinal diseases. Japanese Journal of Clinical Pathology 58(2): 162–68.

Gokce M, Saydam O, Hanci V, Can M and Bahadir B. 2012. Antioxidant vitamins C, E and coenzyme Q10 vs dexamethasone: comparisons of their effects in pulmonary contusion model. Journal of Cardiothoracic Surgery 7: 92. DOI: https://doi.org/10.1186/1749-8090-7-92

Golbidi S, Ebadi S A and Laher I. 2011. Antioxidants in the treatment of diabetes. Current Diabetes Reviews 7: 106–25. DOI: https://doi.org/10.2174/157339911794940729

Hassan N, Sheikh G N, Shaheen M and Willayat M M. 2013. Hemato-biochemical and therapeutic studies on Escherichia coli associated with concurrent enteric infection in lambs. Veterinary World 6: 870–73. DOI: https://doi.org/10.14202/vetworld.2013.870-873

Jain N C. 1986. Schalm’s Veterinary Hematology. 4th edn. Lea and Febiger, Philadelphia, USA.

Kanda T, Fujii H, Tani T, Murakami H, Suda T and Sakai Y. 1996. Intestinal fatty acid binding protein is a useful diagnostic marker for mesenteric infarction in humans. Gastroenterology 11: 339–43. DOI: https://doi.org/10.1053/gast.1996.v110.pm8566578

Khadaroo R G, Fortis S, Salim S Y, Streutker C, Churchill T A and Zhang H. 2014. I-FABP as biomarker for the early diagnosis of acute mesenteric ischemia and resultant lung injury. PLoS One 9(12): e115242. DOI: https://doi.org/10.1371/journal.pone.0115242

Kim Y J, E-H Kim and Hahm K B. 2012. Oxidative stress in inflammation-based gastrointestinal tract diseases: Challenges and opportunities. Journal of Gastroenterology and Hepatology 27: 1004–10. DOI: https://doi.org/10.1111/j.1440-1746.2012.07108.x

Kumar B, Shekhar P and Kumar N. 2010. A clinical study on neonatal calf diarrhoea. Intas Polivet 11: 233–35.

Leal M L R, Fialho S S, Cyrillo F C, Bertagnon H G, Ortolani E L and Benesi F J. 2012. Intravenous hypertonic saline solution (7.5%) and oral electrolytes to treat of calves with noninfectious diarrhea and metabolic acidosis. Journal of Veterinary Internal Medicine 26: 1042–50. DOI: https://doi.org/10.1111/j.1939-1676.2012.00960.x

Lee B J, Huang Y C, Chen S J and Lin P T. 2012. Effects of coenzyme Q10 supplementation on inflammatory markers (high-sensitivity C-reactive protein, interleukin-6, and homocysteine) in patients with coronary artery disease. Nutrition 28: 767–72. DOI: https://doi.org/10.1016/j.nut.2011.11.008

Linnane A W, Kios M and Vitetta L. 2007. Coenzyme Q(10)—its role as a prooxidant in the formation of superoxide anion/ hydrogen peroxide and the regulation of the metabolome. Mitochondrion 7: 51–61. DOI: https://doi.org/10.1016/j.mito.2007.03.005

Lykkesfeldt J and Svendsen O. 2007. Oxidants and antioxidants in disease: oxidative stress in farm animals. The Veterinary Journal 173: 502–11. DOI: https://doi.org/10.1016/j.tvjl.2006.06.005

Malik S, Kumar A, Verma A K, Gupta M K, Sharma S D, Sharma A K and Rahal A. 2013. Haematological profile and blood chemistry in diarrheic calves affected with collibacillosis. Tropical Animal Health and Production 1: 10–14.

McLamb B L, Gibson A J, Overman E L, Stahl C and Moeser A J. 2013. Early weaning stress in pigs impairs innate mucosal immune responses to enterotoxigenic E. coli challenge and exacerbates intestinal injury and clinical disease. PLoS One 8(4): p.e59838. DOI: https://doi.org/10.1371/journal.pone.0059838

Nagy B and Fekete P. 1999. Enterotoxigenic Escherichia coli (ETEC) in farm Animals. Veterinary Research 30: 259–84.

Niewold T A, Meinen M and Van der Meulen J. 2004. Plasma intestinal fatty acid binding protein (I-FABP) concentrations increase following intestinal ischemia in pigs. Research in Veterinary Science 77(1): 89–91. DOI: https://doi.org/10.1016/j.rvsc.2004.02.006

Ottosen H E. 1959. Some statistics on calf mortality. Nordisk Veterinaermedicin 11: 493–512.

Panda D, Patra R C, Nandi S and Swarup D. 2009. Oxidative stress indices in gastroenteritis in dogs with canine parvoviral infection. Research in Veterinary Science 86: 36–42. DOI: https://doi.org/10.1016/j.rvsc.2008.05.008

Prakash S, Sunitha J and Hans M. 2010. Role of coenzyme Q10 as an antioxidant and bioenergizer in periodontal diseases. Indian Journal of Pharmacology 42: 334–37. DOI: https://doi.org/10.4103/0253-7613.71884

Quinn P J, Carter M E, Markey B and Carter G R. 2004. Clinical Veterinary Microbiology. Mosby-Year Book Europe Limited. Lynton House, London, England.

Rajora V S and Pachauri S P. 2000. Laboratory assessment as an aid to rehydration therapy in neonatal diarrhoeic calves. Indian Journal of Veterinary Medicine 20(1): 18–20.

Ranjan R, Naresh R, Patra, R C and Swarup D. 2006. Erythrocyte lipid peroxides and blood zinc and copper concentrations in acute undifferentiated diarrhoea in calves. Veterinary Research Communications 30(3): 249–54. DOI: https://doi.org/10.1007/s11259-006-3185-8

Roffe L, Schmidt K and Ernst E. 2004. Efficacy of coenzyme Q10 for improved tolerability of cancer treatments: a systematic review. Journal of Clinical Oncology 22: 4418– 24. DOI: https://doi.org/10.1200/JCO.2004.02.034

Sahinduran S and Albay M K. 2004. Supplemental ascorbic acid and prevention of neonatal calf diarrhoea. Acta Veterinaria Brunensis 73: 221–24. DOI: https://doi.org/10.2754/avb200473020221

Sastry K V H, Moudgal R P, Mohan J, Tyagi J S and Rao G S. 2002. Spectrophotometric determination of serum nitrite and nitrate by copper-cadmium alloy. Analytical Biochemistry 306: 79–82. DOI: https://doi.org/10.1006/abio.2002.5676

Sawicka E, Dugosz A, Krzysztof P and Guzik A. 2013. The effects of coenzyme q10 and baicalin in Cisplatin-induced lipid peroxidation and nitrosative stress. Acta Poloniae Pharmaceutica Drug Research 70: 977–85.

Singh M, Gupta V K, Mondal D B, Bansal S K, Sharma D K, Shakya M and Gopinath D. 2014. A study on alteration in Haemato-biochemical parameters in Colibacillosis affected calves. International Journal of Advanced Research 2: 746– 50.

Svensson C, Lundborg K, Emanuelson U and Olsson S O. 2003. Morbidity in Swedish dairy calves from birth to 90 days of age and individual calf-level risk factors for infectious diseases. Preventive Veterinary Medicine 58: 179–97. DOI: https://doi.org/10.1016/S0167-5877(03)00046-1

Trevisan M, Browne R, Ram M, Muti P, Freundenheim J, Carosella A N and Armstrong D. 2001. Correlates of markers of oxidative status in the general population. American Journal of Epidemiology 154: 348–56. DOI: https://doi.org/10.1093/aje/154.4.348

Uhde F L, Kaufmann T, Sager H, Albini S, Zanoni R, Schelling E and Meylan M. 2008. Prevalence of four enteropathogens in the faeces of young diarrheic dairy calves in Switzerland. Veterinary Record 163: 362–66. DOI: https://doi.org/10.1136/vr.163.12.362

Verley H, Grawlock A H and Bell M. 1980. Practical Biochemistry. Vol. I, 5th edn, PP 458–84. William Heinmann, Medical book Ltd, London.

Wadhwa D, Wadhwa D R, Kumari M and Katoch B S. 2006. Therapeutic efficacy of enrofloxacin (Quinlntas) in neonatal calf diarrhoea. Intas Polivet 7: 45–46.

Windsant I C V, Hellenthal F A, Derikx J P, Prins M H, Buurman W A, Jacobs M J and Schurink G W H. 2012. Circulating intestinal fatty acid-binding protein as an early marker of intestinal necrosis after aortic surgery: a prospective observational cohort study. Annals of Surgery 255(4): 796–803. DOI: https://doi.org/10.1097/SLA.0b013e31824b1e16

Wybenga D R, Di Giorgio J and Pileggi V J. 1971. Manual and automated methods for urea nitrogen measurement in whole serum. Clinical Chemistry 17(9): 891–95. DOI: https://doi.org/10.1093/clinchem/17.9.891