Cryopreservation of mithun semen: comparative study of conventional vs controlled freezing

M H KHAN; PAPIYA SINHA; P PERUMAL; S B HAZARIKA

doi:10.56093/ijans.v87i6.71082

Authors

M H KHAN National Research Centre on Mithun, Jharnapani, Medziphema, Nagaland 797 106 India
PAPIYA SINHA National Research Centre on Mithun, Jharnapani, Medziphema, Nagaland 797 106 India
P PERUMAL National Research Centre on Mithun, Jharnapani, Medziphema, Nagaland 797 106 India
S B HAZARIKA National Research Centre on Mithun, Jharnapani, Medziphema, Nagaland 797 106 India

https://doi.org/10.56093/ijans.v87i6.71082

Keywords:

Cryopreservation, Freezing methods, Mithun semen, Sperm parameters

Abstract

In this present study, 2 different semen freezing techniques (conventional and controlled freezing) for cryopreservation of mithun semen were compared with regard to post-thaw sperm characteristics. Pooled ejaculates (10) of mithun semen were utilized for the present study. Each ejaculate was aliquoted into 2 and extended with tris egg yolk citrate glycerol (TEYG) extender and freezing was done with conventional method as well as through programmed freezer using 0.5 ml straw. Semen samples were evaluated for sperm motility, liveability, acrosome integrity, plasma-membrane integrity and sperm abnormalities both in fresh and frozen semen. Though there was nonsignificant difference in post-thaw sperm motility and liveability; however, acrosome and plasma-membrane integrity were significantly higher in conventional freezing method as compared to that in controlled freezing method.

Downloads

Download data is not yet available.

References

Bhattacharya H K, Goswami B K, Bujarbaruah K M, Deka B C, Baishya N and Sarma B C. 2005. Characteristics of semen collected by massage method in mithun (Bos frontalis) bulls. Indian Journal of Animal Science 75: 1168– 69.

Bhattacharyya H K, Goswami B K, Bujarbaruah K M, Deka B C and Biswas R K. 2009. Collection and characterization of

semen in mithun (Bos frontalis) bulls. Theriogenology 72(5): 699–703.

Clulow J R, Mansfield L J, Morris L H A, Evans G and Maxwell W M C. 2008. A comparison of freezing methods for the cryopreservation of stallion spermatozoa. Animal Reproduction Science 108: 298–308. DOI: https://doi.org/10.1016/j.anireprosci.2007.08.014

Jayendran R S, Vander-Ven H H, Parez-Pelaez M, Crabo B G and Zaneveld L J D. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. Journal of Reproduction and Fertility 70: 219–28. DOI: https://doi.org/10.1530/jrf.0.0700219

Johnson L A, Weitze K F, Fisher P and Maxwell W M C. 2000. Storage of boar semen. Animal Reproduction Science 62: 143– 72. DOI: https://doi.org/10.1016/S0378-4320(00)00157-3

Kaeoket K, Tantiparinyakul K, Kladkaew W, Chanapiwat P and Techakumphu M. 2008. Effect of different antioxidants on quality of cryopreserved boar semen in different breeds. Thai Journal of Agricultural Science 41(1–2): 1–9.

Karunakaran M, Dhali A, Mech A, Khate K, Rajkhowa C and Mishra D P. 2007. Preservation of mithun (Bos frontalis) semen at refrigeration temperature. Animal Reproduction Science 101(3–4): 257–64. DOI: https://doi.org/10.1016/j.anireprosci.2006.09.011

Kumar S and Bhattacharya H K. 2009. An investigation on certain physical characteristics of semen in mithun. Indian Journal of Animal Sciences 79 (4): 375–76.

Kumar S, Millar J D and Watson P F. 2003. The effect of cooling rate on the survival of cryo preserved bull, ram, and boar spermatozoa: a comparison of two controlled-rate cooling machines. Cryobiology 46: 246–53. DOI: https://doi.org/10.1016/S0011-2240(03)00040-3

Maziero R R D, Guasti P N, Monteiro G A, Avanzi B R, Hartwig F P, Lisboa F P, Martin I and Papa F O. 2012. Evaluation of sperm kinetics and plasma membrane integrity of frozen equine semen in different storage volumes and freezing conditions. Journal of Equine Veterinary Science 33: 165–68. DOI: https://doi.org/10.1016/j.jevs.2012.06.008

Mondal M, Baruah K K, Chatterjee A and Ghosh M K. 2013. Characterization and gene expression profiling of epididymal sperm collected from dead mithun (Bos frontalis) bulls and its preservation. International Journal of Biotechnology and Bioengineering Research 4(6): 535–42.

Mondal M, Karunakaran M, Lee K and Rajkhowa C. 2010. Characterization of Mithun (Bos frontalis) ejaculates and fertility of cryopreserved sperm. Animal Reproduction Science 118: 210–16. DOI: https://doi.org/10.1016/j.anireprosci.2009.09.001

Roca J, Hernandez M, Carvajal G, Vazquez J and Martinez E. 2006. Factors influencing boar sperm survival. Journal of Animal Sciences 84: 2692–99. DOI: https://doi.org/10.2527/jas.2006-094

Salazar J L Jr, Teague S R, Love C C, Brinsko S P, Blanchard T L and Varner D D. 2011. Effect of cryopreservation protocol on post-thaw characteristics of stallion sperm. Theriogenology 76: 409–18. DOI: https://doi.org/10.1016/j.theriogenology.2011.02.016

Sancho S, Casas I, Ekwall H, Saravia F, Rodriguez-Martinez H et al. 2007. Effects of cryopreservation on semen quality and the expression of sperm membrane hexose transporters in the spermatozoa of Iberian pigs. Reproduction 134: 111–21. DOI: https://doi.org/10.1530/REP-07-0118

Watson P F. 1975. Use of Giemsa stain to detect changes in the acrosome of frozen ram spermatozoa. Veterinary Record 97: 12–15. DOI: https://doi.org/10.1136/vr.97.1.12