Standardisation of loop mediated isothermal amplification assay for rapid detection of New Duck disease

Abstract

In the present study, a loop-mediated isothermal amplification assay targeting the ompA gene of R. anatipestifer was standardised using three freeze dried isolates of the organism. The reaction could be completed in a short time i.e. approximately an hour, as compared to PCR which requires about 3 h for completion. The whole isothermal reaction was performed in a simple water bath and the positive reaction could be detected with naked eye, thereby confirming that no expensive equipment is needed for the diagnosis of the pathogen effectively. This technique could be mastered by veterinarians and laboratory technicians very easily compared to PCR; thereby reducing the time lag in reaching a confirmative diagnosis. This assay could be carried out in a local laboratory without any special equipment and high edge facilities.Once properly standardized, the LAMP assay serves as a very simple, rapid and effective pen-side diagnostic technique. It has the potential to revolutionize the field of human and veterinary disease diagnosis, thereby aiding in adopting prompt control and therapeutic strategies.