Molecular characterization and sequence phylogenetic analysis of Babesia bigemina cattle isolate from Mathura based on 18S ribosomal DNA gene

VIKRANT SUDAN; DAYA SHANKAR; BARKHA SHARMA; AMIT KUMAR JAISWAL; AMIT SINGH

doi:10.56093/ijans.v87i8.73470

Authors

VIKRANT SUDAN DUVASU, Mathura, Uttar Pradesh 281 001 India
DAYA SHANKAR DUVASU, Mathura, Uttar Pradesh 281 001 India
BARKHA SHARMA DUVASU, Mathura, Uttar Pradesh 281 001 India
AMIT KUMAR JAISWAL DUVASU, Mathura, Uttar Pradesh 281 001 India
AMIT SINGH DUVASU, Mathura, Uttar Pradesh 281 001 India

https://doi.org/10.56093/ijans.v87i8.73470

Keywords:

Babesia bigemina, Phylogenetic analysis, 185 ribosomal DNA

Abstract

VSG is considered to be an important molecule for both molecular and serological based detection of surra. Of late, there are reports of variation and/or absence of RoTat 1.2 evansi VSG from a fewer isolates of murine and wild life origin. Hence, in order to use VSG for routine diagnosis of trypanosomiosis, there is need for selection of that portion of gene, which is conserved in all trypanosomes irrespective of species and geographical distribution. Hence, we identified a 205 bp portion of VSG, which is conserved in Trypanosomes.The present communication deals with molecular cloning and characterization of this Trypanozoan specific VSG gene from T. evansi of horse origin from semi arid region of India and checked its uniqueness by comparing it with various other isolates across the world. The selected truncated showed cent percent homology with all other isolates, thereby, justifying its uniqueness.

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References

Claes F, Radwanska M, Urakawa T, Majiwa P, Goddeeris B, Büscher P. 2004. Variable Surface Glycoprotein RoTat 1.2 PCR as a specific diagnostic tool for the detection of Trypanosoma evansi infections.Kinetoplastid Biology and Disease 1–6.

Gadelha C, Holden J M, Allison H C, Field M C and Jennifer M. 2011. Spe-cializations in a successful parasite: what makes the blood streamform African trypanosomes so deadly? Molecular and Biochemical Parasitology 179: 51–58. DOI: https://doi.org/10.1016/j.molbiopara.2011.06.006

Jia Y, Zhao X, Zou J and Suo X. 2011. Trypanosoma evansi: Identification and characterization of a variant surface glycoprotein lacking cysteine residues in its C-terminal domain. Experimental Parasitology 127: 100–106. DOI: https://doi.org/10.1016/j.exppara.2010.06.035

Ngaira J M, Njagi E N, Ngeranwa J J and Olembo N K. 2004. PCR amplifica-tion of RoTat 1.2 VSG gene in Trypanosoma evansi isolates in Kenya. Veterinary Parasitology 120: 23– 33. DOI: https://doi.org/10.1016/j.vetpar.2003.12.007

Salim B, Bakheit M A, Kamau J, Nakamura I and Sugimoto C. 2011.Molecular epidemiology of camel trypanosomiasis based on ITS1rDNA and RoTat 1.2 VSG gene in the Sudan. Parasite and Vectors 4: 31. DOI: https://doi.org/10.1186/1756-3305-4-31

Sambrook J, Russell D. 2001. Molecular Cloning: A Laboratory Manual.Cold Spring Harbor Laboratory Press, New York.

Turner M J, Cardoso de Almeida M L, Gurnett A M, Raper J and Ward J. 1985. Biosynthesis, attachment and release of variant surface glyco-proteins of the African trypanosome. Current Topics in Microbiology Immunology 117: 23–55. DOI: https://doi.org/10.1007/978-3-642-70538-0_2

Verloo D, Holland W, My L N, Thanh N G, Tam P T, Goddeeris B, Vercruysse J and Uscher P. 2000. Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from North Vietnam. Veterinary Parasitology 29: 87–96. DOI: https://doi.org/10.1016/S0304-4017(00)00284-3

Verloo D, Magnus E and Buscher P. 2001. General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin. Veterinary Parasitology 97: 183–89. DOI: https://doi.org/10.1016/S0304-4017(01)00412-5

Zhang Z Q and Baltz T. 1994. Identification of Trypanosoma evansi. Trypanosoma equiperdum and Trypanosoma brucei brucei using repetitive DNA probes. Veterinary Parasitology 53: 197–208. DOI: https://doi.org/10.1016/0304-4017(94)90183-X