B1 gene based semi nested PCR for detection of toxoplasmosis from poultry hearts
DOI:
https://doi.org/10.56093/ijans.v87i8.73473Keywords:
B1 amplicon, Semi nested PCR, Tissue samples, ToxoplasmosisAbstract
The WTO guidelines on control strategies regarding food-borne diseases insist on mandatory serological investigations of the causative agent(s) at the farm level and in slaughtered animals. But in countries like India, it is very difficult to go for serological based diagnosis on slaughtered meat found in local markets. Hence, in these cases, PCR remains the sole source of detection of infectious agents. The aim of the study was to find out the presence of toxoplasmosis in meat samples of poultry reared under semi arid conditions of Mathura. The samples were subjected to semi nested PCR, using primers directed against the multicopy of universal B1 gene. Out of 40 heart samples screened, 2 were found to be positive for Toxoplasma gondii DNA.
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