Molecular characterization and phylogenetic sequence analysis of unique conserved portion of VSG of Trypanosoma evansi
DOI:
https://doi.org/10.56093/ijans.v87i8.73582Keywords:
Molecular characterization, Trypanosoma evansi, VSGAbstract
The present communication deals with the molecular characterization and phylogenetic relationship of 18S ribosomal DNA of B. bigemina from cattle of Mathura with other isolates across the globe. The 446 bp product PCR amplified and was cloned into TA vector. Upon sequencing, the nucleotide homology was established and phylogenetic relationship was calculated with other B. bigemina isolates from India and across the world. The present isolate was more close to Argentina and Kenya isolates than the isolates from China, Mexico, Australia and Zimbabwe. The finding is important from the molecular phylogeny point of view.
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Laha R, Das M, Goswami A and Singh P. 2012a. Losses of milk production due to Babesia bigemina infection in a crossbred cow- A case study. Journal of Protozoology Research 22: 6– 9.
Laha R, Das M, Goswami A and Singh P. 2012b. A clinical case of Babesiosis in a crossbred cow of Meghalaya. Indian Journal of Animal Research 46: 302–05.
Laha R, Mondal B, Biswas S K, Chand K, Das M, Sarma D, Goswami A and Sen A. 2015. Detection of Babesia bigemina infection in cattle from north-eastern India by polymerase chain reaction and its genetic relatedness with other isolates. Tropical Animal Health and Production. 47: 633–36. DOI: https://doi.org/10.1007/s11250-015-0769-8
Sambrook J, Fritch E F and Maniatis T. 1989. Molecular cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor Laboratory Press, New York.
Saravanan B C, Das S, Siju S J, Tewari A K, Sankar M, Kataktalware M A and Ramesha K P. 2013. Babesia bigemina infection in yak (Poephagus grunniens L.): Molecular detection and characterization. Veterinary Parasitology 194: 58–64. DOI: https://doi.org/10.1016/j.vetpar.2012.12.024
Sudan V, Jaiswal A K, Parashar R and Shanker D. 2015. A duplex PCR based assay for simultaneous detection of Trypanosoma evansi and Theileria annulata infections in water buffaloes. Tropical Animal Health and Production 47: 915–19. DOI: https://doi.org/10.1007/s11250-015-0808-5
Yin H, Liu G Y, Luo J X, Guan G Q, Ma M L, Ahmed J and Bai Q. 2004. Phylogenetic analysis of Theileria species transmitted by Haemaphysalis qinghaiensis. Parasitology Research 92: 36–42. DOI: https://doi.org/10.1007/s00436-003-0900-z
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