Comparative evaluation of blood based lateral flow assay for diagnosis of brucellosis in livestock species

KAVYA BATLAHALLY ANJANEYAREDDY; VEEREGOWDA BELAMARANAHALLI MUNIVEERAPPA; SRIKRISHNA ISLOOR; LEENA GOWDA; CHIKMANGALURANSAR KAMRAN; AMITHA REENA GOMES; TRIVENI KALLESHAMURTHY; PADMASHREE B SHANKARANARAYANA; RAJESWARI SHOME

doi:10.56093/ijans.v87i9.74286

Authors

KAVYA BATLAHALLY ANJANEYAREDDY ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
VEEREGOWDA BELAMARANAHALLI MUNIVEERAPPA ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
SRIKRISHNA ISLOOR ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
LEENA GOWDA ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
CHIKMANGALURANSAR KAMRAN ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
AMITHA REENA GOMES ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
TRIVENI KALLESHAMURTHY
PADMASHREE B SHANKARANARAYANA ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India
RAJESWARI SHOME ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India

https://doi.org/10.56093/ijans.v87i9.74286

Keywords:

Brucellosis, Blood-LFA, RBPT, Serum-LFA

Abstract

Brucellosis is a zoonotic and economically important disease of livestock. Pen side diagnostics are the need of the hour for the rapid diagnosis of brucellosis. The present study aimed to evaluate blood based lateral flow assay (LFA) with serum based LFA and rose bengal plate test (RBPT) for diagnosis of brucellosis. Sera/blood samples [792: cattle, 153; buffalo, 55; sheep, 140; goats, 219; and pigs, 225] were collected from Karnataka, India with history of repeat breeding, retention of placenta and abortion.The seropositivity of brucellosis in cattle, buffalo, sheep, goats and pigs was 7.84, 3.63, 12.85, 2.73 and 39.11% by RBPT; 5.88, 3.63, 8.57, 2.73 and 31.1% by blood-LFA; and 7.18, 3.63, 10.71, 3.19 and 32.88% by serum-LFA. Relative specificity of blood based LFA was between 98.97 to 99.53% and 97.42 to 100% in comparison to serum-LFA and RBPT, respectively in all the species. The relative sensitivity for all the species ranged from 64.29 to 83.33% by blood-LFA and 57.14 to 84.09% by serum-LFA. The diagnostic accuracy of blood-LFA to RBPT and serum- LFA ranged from 91 to 99% among the species studied. It is opined that blood based LFA can serve as diagnostic test in place of serum based LFA for brucellosis screening in multiple livestock species and suggested for adaptation on-farm, slaughter, market, clinical and prepurchase surveillance of brucellosis in the country.

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Author Biography

TRIVENI KALLESHAMURTHY

Senior Research Fellow, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka 560 064 India

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