Masculinization of dwarf gourami Trichogaster lalius through immersion treatment of 17α-methyltestosterone
350 / 114
Keywords:
17α-methyltestosterone, Gonadosomatic index, Hormonal profiling, Masculinization, Progeny testing, Trichogaster laliusAbstract
The present study was conducted to study the effect of 17α-methyltestosterone (17α-MT) on masculinization
efficiency through immersion treatment in dwarf gourami, Trichogaster lalius. The immersion treatment of 17α-
MT at doses of 250, 500, 750 and 1000 μg/l was carried out for 3 h daily on third, fifth and eighth day after hatching. The highest concentrations of 17α-MT produced the highest percentage of males (84.29%). The progeny testing of males from17α-MT treated groups indicated that the female progenies of each of the males tested differ significantly from that of control, indicating that all those males carried XX genotype. The gonado-somatic index
of the hormone treated fish revealed significant suppression of the ovarian development.
Downloads
References
Bhandari R K, Alam M A, Soyano K and Nakamura M. 2006.Induction of female-to–male sex change in the honeycomb grouper (Epinephelus merra) by 11- ketotestosterone treatments. Zoological Science 23: 65–69. DOI: https://doi.org/10.2108/zsj.23.65
Bharadwaj R and Sharma L L. 2000. Effect of methyl testosterone (tablets) in sterilization and masculnization of common carp, Cyprinus carpiocommunis (L). Indian Journal of Fisheries 47(4): 377–81.
Cogliati K M, Corkum L D and Doucet S M. 2010. Bluegill coloration as a sexual ornament: evidence from ontogeny, sexual dichromatism, and condition dependence. Ethology 116: 416–28. DOI: https://doi.org/10.1111/j.1439-0310.2010.01755.x
Galbreath P F, Adams N D and Sherrill L W III. 2003. Successful sex reversal of brook trout with 17α-methyl dihydro testosterone treatments. North American Journal of Aquaculture 65(3): 235–39. DOI: https://doi.org/10.1577/C02-039
Gale W L, Fitzpatrick M S, Lucero M, Contreras-Sanchez W M and Schreck C B.1999. Masculinization of Nile tilapia (Oreochromis niloticus) by immersion in androgens. Aquaculture 178: 349–57. DOI: https://doi.org/10.1016/S0044-8486(99)00136-2
George T and Pandian T J. 1996. Hormonal induction of sex reversal and progeny testing in the zebra cichlid. Cichlaso manigrofasciatum. Journal of Experimental Zoology 275: 374– 82. DOI: https://doi.org/10.1002/(SICI)1097-010X(19960801)275:5<374::AID-JEZ6>3.0.CO;2-M
Gomelsky B I, Mism S D, Onders R J and Bean W B. 2002. Hormonal sex reversal and evidence of female homogametic in black crappie. North American Journal of Aquaculture 290: 177–81.
Haniffa M A, Sridhar S and Nagarajan M. 2004.Hormonal manipulation of sex in stinging catfish Heteropneustes fossilis (Bloch).Current Science 86(7): 1012–17.
Haugen T, Andersson E, Norberg B and Taranger G L. 2011.The production of hermaphrodites of Atlantic cod (Gadus morhua) by masculinization with orally administered 17–α-methyl testosterone, and subsequent production of all-female cod populations. Aquaculture 311: 248–54. DOI: https://doi.org/10.1016/j.aquaculture.2010.12.003
James R and Sampath K. 2006. Effect of dietary administration of methyltestosterone on the growth and sex reversal of two ornamental fish species. Indian Journal of Fisheries 53(3): 283–90.
Jessy D and Varghese T J.1988.Hormonal sex control in Bettasplendens (Regan) and Xiphophorushelleri (Heckel). Proceeding of 1st Indian Fisheries Forum. M. Mohan Joseph (Ed.), Asian Fisheries Society, Indian Branch, Mangalore, pp 123–24.
Joshi H D. 2013. ‘Preparation and characterization of fadrozole loaded nanoparticles for masculinization of Poecili areticulata (Peters, 1859).’ M.F.Sc. thesis submitted to Central Institute of Fisheries Education, Mumbai, pp.111.
Kirankumar S and Pandian T J. 2002. Effect on growth and reproduction of hormone immersed and masculinized fighting fish Betta splendens. Journal of Experimental Zoology 293: 606–16. DOI: https://doi.org/10.1002/jez.10181
Kumar A and Haniffa M A K. 2011. Effect of 17α methyltestosterone on sex reversal of Xiphophorous maculatus platy and Cyprinus carpio Koi carp. Journal of Research in Biology 8: 580–86.
Lee P, King H and Pankhurst N. 2004. Preliminary assessment of sex inversion of farmed Atlantic Salmon by dietary and immersion androgen treatments. North American Journal of Aquaculture 66(1): 1–7. DOI: https://doi.org/10.1577/A03-015
Mousavi-Sabet H. 2011. The effect of 17α-methyl testosterone on masculinization, mortality rate and growth in convict cichlid (Cichlasomanigro fasciatum). World Journal of Fish and Marine Sciences 3(5): 422–26.
Pandian T J and Sheela S G. 1995.Hormonal induction of sex- reversal in fish. Aquaculture 138: 1–22. DOI: https://doi.org/10.1016/0044-8486(95)01075-0
Piferrer F and Lim L C. 1997.Application of sex reversal technology in ornamental fish culture.Aquarium Sciences and Conservation 1: 113–18. DOI: https://doi.org/10.1023/A:1018391702814
Sarter K, Papadaki M, Zanuy S and Mylonas C C. 2006. Permanent sex inversion in 1-year-old juveniles of the protogynous dusky grouper (Epinephelus marginatus) using controlled-release 17α-methyl testosterone implants. Aquaculture 256: 443–56. DOI: https://doi.org/10.1016/j.aquaculture.2006.01.034
Silarudee S and Kongchum P. 2008. Masculinization of flowerhorn by immersion in androgens. Silpakorn University Science and Technology Journal 2(2): 26–32.
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2017 The Indian Journal of Animal Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Animal Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.