Associations of genetic variations in the OCX-32 gene with egg production traits in Korean native chickens

JI-YEON LEE; SO-YOUNG CHOI; NARESH KUMAR SINGH; SONG-YI YANG; MIN-WOOK HONG; DONG KEE JEONG; YEONG HO HONG; SUNG-JIN LEE

doi:10.56093/ijans.v87i9.74321

Authors

JI-YEON LEE Kangwon National University, Chuncheon 24341, Republic of Korea
SO-YOUNG CHOI Kangwon National University, Chuncheon 24341, Republic of Korea
NARESH KUMAR SINGH Kangwon National University, Chuncheon 24341, Republic of Korea
SONG-YI YANG Kangwon National University, Chuncheon 24341, Republic of Korea
MIN-WOOK HONG Kangwon National University, Chuncheon 24341, Republic of Korea
DONG KEE JEONG Kangwon National University, Chuncheon 24341, Republic of Korea
YEONG HO HONG Kangwon National University, Chuncheon 24341, Republic of Korea
SUNG-JIN LEE Kangwon National University, Chuncheon 24341, Republic of Korea

https://doi.org/10.56093/ijans.v87i9.74321

Keywords:

Association, Egg production trait, Korean native chicken, OCX-32, SNP

Abstract

The present study was undertaken to find out novel single nucleotide polymorphism (SNPs) and to analyze the association between SNPs of OCX-32 gene and egg production traits, in the four Korean native chicken breeds (Ogol, black, gray, and white). Twenty-one variations (16 SNPs and 5 INDELs) in the intronic region of the OCX-32 gene were detected, including new 9 variants (1500T>A, 1504A>G, 1658A>G, 1668A>T, 1821A>C, 1234 del A, ins CTT, ins TTC, and 1681 del T) in the chicken population (n=120). Fifteen variations (1346A>G, 1373G>A, 1399T>C, 1446C>G, 1500T>A, 1504A>G, 1522G>A, 1530T>A, 1563G>A, 1668A>T, 1743G>T, 1772T>A, 1821A>C, ins TTC, and ins TCT) showed significant association with egg production ratio, weight and age in the chickens. Conclusively, the result obtained suggested that SNPs and INDELs of the OCX-32 gene might be useful as genetic markers for egg production traits in breeding program of the Korean native chicken.

Downloads

Download data is not yet available.

References

Lee J Y, Choi S Y, Kim C D, Hong Y H, Jeong D K and Lee S J. 2014. Effects of c.494A>C and c.267T>G SNPs in OCX-32 gene of Korean native chicken on egg production traits. Poultry Science 41: 191–96. DOI: https://doi.org/10.5536/KJPS.2014.41.3.191

Maxwell T H, Yung-ching C, Louis C G and Marc D M. 2008. Colloidal-gold immunocytochemical localization of osteopontin in avian egg shell gland and egg shell. Journal of Histochemistry and Cytochemistry 56(5): 467–76. DOI: https://doi.org/10.1369/jhc.2008.950576

Naha B C, Boro P, Prakash C, Sailo L, Prasad A, Chaudhary R and Prakash O P. 2016. Concepts of whole genome sequencing and SNP markers in poultry breeding-A review. International Journal of Research and Current Development 2(5): 109–10.

Takahashi H, Yang D, Sasaki O, Furukawa T and Nirasawa K. 2009. Mapping of quantitative trait loci affecting eggshell quality on chromosome 9 in an F2 intercross between two chicken lines divergently selected for eggshell strength. Animal Genetics 40: 779–82. DOI: https://doi.org/10.1111/j.1365-2052.2009.01914.x

Uemoto Y, Suzuki C, Sato S, Sato S, Ohtake T, Sasaki O, Takahashi H and Kobayashi E. 2009. Polymorphism of the ovocalyxin-32 gene and its association with egg production traits in the chicken. Poultry Science 88: 2512–17. DOI: https://doi.org/10.3382/ps.2009-00331

Wu X, Liu X T, Lan J J, Wan X P, Yan M J, Lian S Y and Li A. 2016. Investigation of insulin-like growth factor–1 gene with egg-laying traits in the Muscovy duck (Cairina moschata). Canadian Journal of Animal Science 96(2): 203–14. DOI: https://doi.org/10.1139/cjas-2015-0024

Xiao S, Wang P, Dong L, Zhang Y, Han Z, Wang Q and Wang Z. 2016. Whole genome single nucleotide polymorphism (SNP) marker discovery and association analysis with eicosapetaenoic acid (EPA) and docosahexaenoic acid (DHA) content in Larimichthys crocea. Peer Journal 4: e2664; DOI 10.7717/peerj.2662. DOI: https://doi.org/10.7717/peerj.2664