Effect of hydro-alcoholic extract of Rhodiola imbricata on growth performance, immunomodulation, antioxidant level and blood biochemical parameters in broiler chickens at high altitude cold desert


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Authors

  • SAHIL KALIA Defence Institute of High Altitude Research (DIHAR), DRDO, Leh-Ladakh, Jammu and Kashmir 194 101 India
  • VIJAY KUMAR BHARTI Defence Institute of High Altitude Research (DIHAR), DRDO, Leh-Ladakh, Jammu and Kashmir 194 101 India
  • ARUP GIRI Defence Institute of High Altitude Research (DIHAR), DRDO, Leh-Ladakh, Jammu and Kashmir 194 101 India
  • PRINCE VIVEK Defence Institute of High Altitude Research (DIHAR), DRDO, Leh-Ladakh, Jammu and Kashmir 194 101 India
  • BHUVNESH KUMAR Defence Institute of High Altitude Research (DIHAR), DRDO, Leh-Ladakh, Jammu and Kashmir 194 101 India
  • MALAIRAMAN UDAYABANU Defence Institute of High Altitude Research (DIHAR), DRDO, Leh-Ladakh, Jammu and Kashmir 194 101 India

https://doi.org/10.56093/ijans.v87i10.75244

Keywords:

Broiler, Growth, High altitude, Immune responses, Rhodiola imbricata

Abstract

Extremes of climate and hypobaric hypoxia cause poor growth performance in broiler chickens at high altitude.
The current study was designed to investigate the effect of hydro-alcoholic extract of Rhodiola imbricata on
antioxidant, cytokines, blood biochemical and growth performance of broilers at high altitude (3500 m). For invivo
study, one day-old broiler chicks of average initial body weight 36.40±0.42 g were randomly assigned to seven groups in three replicates (10 chicks in each replicate) as per completely randomized design. Experimental groups included control (fed basal diet), and treatment T1, T2, T3, T4, T5, and T6 which received hydro-alcoholic extract of Rhodiola imbricata in drinking water @ 100, 150, 200, 300, 400, and 800 mg/kg body weight of chicken respectively, along with basal diet. Blood samples were collected at 0, 21st, and 42nd day. HPLC analysis of extract revealed the presence of salidroside and p-tyrosol. As a result of this study, birds in T5 group had significantly higher body weight as compared to other groups. Furthermore, they had significantly higher total antioxidant capacity, free radical scavenging activity, interleukin-2, total protein, globulin, HDL level and lower malondialdehyde, interleukin-6, cholesterol, triglyceride, LDL, glucose, A/G, ALT, AST level as compared to control group. Our results suggest that, Rhodiola imbricata extract @ 400 mg/kg body weight of chicken, exhibited beneficial effect on growth performance and therefore, can be used as a phytogenic feed additive for broiler chickens.

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References

Ahmed M, Henson D A, Sanderson M C, Nieman D C, Zubeldia J M and Shanely R A. 2015. Rhodiola rosea exerts antiviral activity in athletes following a competitive marathon race. Frontiers in Nutrition 2: 1–7.

Ali M M. 2015. Emerging prospective of Indian livestock: A study on poultry industry. Asian Journal of Managerial Science 4: 33–39.

Arora R, Chawla R, Sagar R, Prasad J, Singh S and Kumar R. 2005. Evaluation of radioprotective activity of Rhodiola imbricata – a high altitude plant. Molecular and Cellular Biochemistry 273: 209–23.

Ballabh B and Chaurasia O P. 2007. Traditional medicinal plants of cold desert Ladakh-used in treatment of cold, cough, and fever. Journal of Ethnopharmacology 112: 341–49.

Balog J M, Anthony N B, Cooper M A, Kidd B D, Huff G R, Huff W E and Rath N C. 2000. Ascites syndrome and related pathologies in feed restricted broilers raised in a hypobaric chamber. Poultry Science 79: 318–23.

Benzie I F and Strain J J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”. The FRAP assay. Analytical Biochemistry 239: 70–76.

Biswas A, Bharti V K, Deshmukh P B, Venkatesan G and Srivastava R B. 2011. Commercial poultry farming in cold arid region of Leh-Ladakh. Innovatives in Agro Animal Technologies. pp. 216–233. (Eds) Srivastava R B and Selvamurthy W. Satish Serial Publishing House, New Delhi.

Boyman O and Sprent J. 2012. The role of interleukin-2 during homeostasis and activation of immune system. Nature Review Immunology 12: 180–90.

Brand-Williams W, Cuvelier M E and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 28: 25–30.

Buege J A and Aust S D. 1978. The thiobarbuturic acid assay. Methods in Enzymology 52: 306–07.

Calcabrini C, De Bellis R, Mancini U, Cucchiarini L, Potenza L, De Sanctis R, Patrone V, Scesa C and Dacha M. 2010. Rhodiola rosea ability to enrich cellular antioxidant defences of cultured human keratinocytes. Archives of Dermatological Research 302: 191–200.

Chiang H M, Chen H C, Wu C S, Wu P Y and Wen K C. 2015. Rhodiola plants: Chemistry and biological activities. Journal of Food and Drug Analysis 23: 359–69.

Gengaihi S E, Ella F A E, Emad M H, Shalaby E and Doha H. 2014. Antioxidant activity of phenolic compounds from different grape wastes. Journal of Food Processing and Technology 5: 1–5.

Ishaque S, Shamseer L, Bukutu C and Vohra S. 2012. Rhodiola rosea for physical and mental fatigue: a systematic review. BMC Complementary and Alternative Medicine 12: 1–9.

Kala C P. 2005. Health traditions of Buddhist community and role of amchis in trans-Himalayan region of India. Current Science 89: 1331–38.

Kala C P. 2006. Medicinal plants of the high altitude cold desert in India: diversity, distribution and traditional uses. International Journal of Biodiversity Science and Management 2: 43–56.

Kalia S, Bharti V K, Gogoi D, Giri A and Kumar B. 2017. Studies on the growth performance of different broiler strains at high altitude and evaluation of probiotic effect on their survivability. Scientific Reports. Article ID 46074. doi: 10.1038/srep46074.

Kayser B and Verges S. 2013. Hypoxia, energy balance and obesity: from pathophysiological mechanisms to new treatment strategies. Obesity Reviews 14: 579–92.

Li D, Fu Y, Zhang W, Su G, Liu B, Liang D, Liu Z, Zhang X, Cao Y, Zhang N and Yang Z. 2013. Salidroside attenuates inflammatory responses by suppressing nuclear factor kappaB and mitogen activated protein kinases activation in lipopolysaccharide-induced mastitis in mice. Inflammation Research 62: 9–15.

Li L, Wang W and Zhao X. 2014. Effect of Rhodiola on production, health and gut development of broilers reared at high altitude in Tibet. Scientific Reports. Article ID 7176. doi: 10.1038/srep07176.

Miller L E, McGinnis G R, Kliszczewicz B, Slivka D, Hailes W, Cuddy J, Dumke C, Ruby B and Quindry J C. 2013. Blood oxidative-stress markers during a high-altitude trek. International Journal of Sport Nutrition and Exercise Metabolism 23: 65–72.

Mishra K P, Ganju L and Singh S B. 2012. Anti-cellular and immunomodulatory potential of aqueous extract of Rhodiola imbricata rhizome. Immunopharmacology and Immunotoxicology 34: 513–18.

Panossian A and Wikman G. 2010. Effect of adaptogens on the central nervous system and their molecular mechanism associated with their stress-protective activity. Pharmaceuticals 3: 188–224.

Panossian A, Wikman G and Sarris J. 2010. Rosenroot (Rhodiola rosea): traditional use, chemical composition, pharmacology and clinical efficacy. Phytomedicine 17: 481–93.

Qian E W, Ge D T and Kong S K. 2012. Salidroside protects human erythrocytes against hydrogen peroxide-induce apoptosis. Journal of Natural Products 75: 531–37.

Radomska-Lesniewska D M, Skopinski P, Balan B J, Bialoszewska A, Jozwiak J, Rokicki D, Skopinska-Rozewska E, Borecka A and Hevelke A. 2015. Angiomodulatory properties of Rhodiola spp. and other natural antioxidants. Central European Journal of Immunology 40: 249–62.

Ramakrihna A and Ravishankar G A. 2011. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling and Behavior 6: 1720–31.

Saunders D, Poppleton D, Struchkov A and Ireland R. 2014. Analysis of five bioactive compounds from naturally occurring Rhodiola rosea in eastern Canada. Canadian Journal of Plant Science 94: 741–48.

Senthilkumar R, Chandran R and Parimelazhagan T. 2014. Hepatoprotective effect of Rhodiola imbricata rhizome against paracetamol-induced liver toxicity in rats. Saudi Journal of Biological Sciences 5: 409–16.

Senthilkumar R, Parimelazhagan T, Chaurasia O P and Srivastava R B. 2013. Free radical scavenging property and antiproliferative activity of Rhodiola imbricata Edgew extracts in HT-29 human colon cancer cells. Asian Pacific Journal of Tropical Medicine 6: 11–19.

SPSS Inc. Released 2008. SPSS Statistics for Windows, Version 17.0. Chicago.

Xu J and Li Y. 2012. Effects of salidroside on exhaustive exercise induced oxidative stress in rats. Molecular Medicine Reports 6: 1195–98.

Zhang B C, Li W M, Guo R and Xu Y W. 2012. Salidroside decreases atherosclerotic plaque formation in low density lipoprotein receptor-deficient mice. Evidence Based Complementary and Alternative Medicine. Article ID 607508. doi: 10.1155/2012/607508.

Zhou Q, Yin Z P, Zhao W, Haq H W and Li H L. 2014. Free radical scavenging activities of oligomeric proanthocyanidins from Rhodiola rosea L. and its antioxidant effects in vivo. Natural product Research 28: 2301–03.

Zhou T, Zheng J, Zhou Y, Li S, Gan R Y and Li H B. 2015. Chemical components and bioactivities of Rhodiola rosea. International Journal of Traditional and Natural Medicines 5: 23–51.

Submitted

2017-10-23

Published

2017-10-25

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Section

Articles

How to Cite

KALIA, S., BHARTI, V. K., GIRI, A., VIVEK, P., KUMAR, B., & UDAYABANU, M. (2017). Effect of hydro-alcoholic extract of Rhodiola imbricata on growth performance, immunomodulation, antioxidant level and blood biochemical parameters in broiler chickens at high altitude cold desert. The Indian Journal of Animal Sciences, 87(10), 1200–1206. https://doi.org/10.56093/ijans.v87i10.75244
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