Virulence genes and antibiotic resistance profile of shiga toxin producing Escherichia coli and enterotoxigenic Escherichia coli from diarrhoeic cattles and human handlers in Asom (India)

LALHRUAIPUII; H RAHMAN; G K SAIKIA; J L KATARIA; LALLAWMZUALI

doi:10.56093/ijans.v87i10.75266

Authors

LALHRUAIPUII ICAR-RC for NEH Region, Umiam, Barapani, Meghalaya 793 103 India
H RAHMAN ICAR-RC for NEH Region, Umiam, Barapani, Meghalaya 793 103 India
G K SAIKIA ICAR-RC for NEH Region, Umiam, Barapani, Meghalaya 793 103 India
J L KATARIA ICAR-RC for NEH Region, Umiam, Barapani, Meghalaya 793 103 India
LALLAWMZUALI ICAR-RC for NEH Region, Umiam, Barapani, Meghalaya 793 103 India

https://doi.org/10.56093/ijans.v87i10.75266

Keywords:

Antibiotic resistance, Enterotoxigenic E. coli, Shiga toxin, Virulence genes

Abstract

The present study was undertaken to isolate and characterize STEC and ETEC from diarrhoeic calves and humans in Asom, India. Out of the 134 presumptive Escherichia coli isolates picked from 80 diarrhoeic calves, 31 isolates could be serotyped into 23 different serogroups; and out of 19 strains of E. coli from human beings, 13 strains could be serotyped into 10 different serogroups. O157 from cattle and O131 from humans were the frequently encountered serogroups. The virulence gene profile of the STEC and ETEC isolates showed that the prevalence of stx2 gene and the emergence of multi drug resistant STEC and ETEC isolates in high proportion indicates that they may act as an important reservoir posing a possible threat to public health and may complicate future therapeutic options.

Downloads

Download data is not yet available.

References

Amaechi N, Abbey S D and Achi O K. 2015. Plasmid profile and antimicrobial resistance ratings of Escherichia coli isolates from pigs and poultry birds in Abia state, Nigeria. International Journal of.Current Microbiology and Applied Sciences 2: 834– 42. DOI: https://doi.org/10.4314/ajcem.v16i2.2

Bauer A W, Kirby W M, Sherriz J C and Tuck N. 1966. Antibiotic susceptibility testing by standardized single disc method. American Journal of Clinical Pathology 45: 493–96. DOI: https://doi.org/10.1093/ajcp/45.4_ts.493

Cai Y, Yordan M A,Yao Q, Li Y and Yu L. 2016. Review of enterotoxigenic Escherichia coli enterotoxins. Academia Journal of Microbiology Research 4: 5–8.

Islam M A, Rahman M, Nahar A, Khair A and Alam M M. 2015. Investigation of pathogenic Escherichia coli from diarrheic calves in selective area of Bangladesh. Bangladesh Journal of Veterinary Medicine 13: 45–51. DOI: https://doi.org/10.3329/bjvm.v13i1.23716

Kataria J L, Dutta T K, Roychoudhury P and Tiwari J G. 2014. Detection and molecular characterization of Shiga toxin producing Escherichia coli and enteropathogenic Escherichia coli from piglets in Mizoram, India. Journal of Pure and Applied Microbiology 8: 2383–88.

Oloyede A R, Afolabi O R and Olalowo O S. 2016. Molecular detection of virulence genes and antibiotic resistance patterns of Escherichia coli O157:H7 isolated from raw beef sold in Abeokuta, South-West Nigeria. Nigerian Journal of Biotechnology 31: 15 –21. DOI: https://doi.org/10.4314/njb.v31i1.3

Quinn P J, Carter M E, Markey B and Carter G R. 2004. Clinical Veterinary Microbiology. Mosby International, London, pp. 209–236.

Rahal E A, Fadlallah S M, Nassar F J, Kazzi N and Matar G M. 2015. Approaches to treatment of emerging Shiga toxin- producing Escherichia coli infections highlighting the O104:H4 serotype. Frontiers in Cellular and Infection Microbiology 5: 24. DOI: https://doi.org/10.3389/fcimb.2015.00024

Rahman H. 2002. Multiplex PCR for detection of stx genes of Escherichia coli. Indian Journal of Medical Research 115: 251–54.

Tahamtan Y, Hayati M and Namavari M M. 2010. Prevalence and distribution of the stx1, stx2 genes in Shiga toxin producing E. coli (STEC) isolates from cattle. Iranian Journal of Microbiology 2: 9–14.