In-vitro digestibility studies with recombinant Saccharomyces cerevisiae expressing fibre degrading enzyme genes

K VIJAYARANI; R ANUPRIYA; A THULASI; M CHANDRASEKHARAIAH

doi:10.56093/ijans.v87i10.75309

Authors

K VIJAYARANI Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
R ANUPRIYA Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
A THULASI Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India
M CHANDRASEKHARAIAH Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu 600 007 India

https://doi.org/10.56093/ijans.v87i10.75309

Keywords:

Exoglucanase, Fibre degrading enzymes, In vitro digestibility, Recombinant endoglucanase, Saccharomyces cerevisiae

Abstract

The present study described the in vitro digestibility of recombinant exo- and endo-glucanase genes of Phanerochaete chrysosporium cloned into pYESDEST-52 vetor and expressed in Saccharomyces cerevisiae. In-vitro RUSITEC trials indicated that recombinant exoglucanase increased the total microbial protein at 24 and 48 h. Overall increase in the in vitro digestibility was observed with both endoglucanase and exoglucanase recombinants. Both the recombinants produced significantly high levels of propionic and acetic acid.

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