Expression of Echinococcus granulosus recombinant EgAg5-38 sub-unit and P-29 antigens and evaluation of their diagnostic potential for cystic echinococcosis in buffaloes

MARY NISHA TIGGA; AJAYTA RIALCH; A ARUN; S SAMANTA; NAVNEET KAUR; R K BAURI; Z A GANAIE; ANDLEEB AFTAB; O K RAINA

doi:10.56093/ijans.v87i11.75839

Authors

MARY NISHA TIGGA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
AJAYTA RIALCH ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A ARUN ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
S SAMANTA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
NAVNEET KAUR ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
R K BAURI ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
Z A GANAIE ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ANDLEEB AFTAB ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
O K RAINA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v87i11.75839

Keywords:

Buffalo, Diagnosis, Echinococcus granulosus, EgAg5-38 sub-unit, IgG-ELISA, P-29 antigen

Abstract

There are no routine, reliable diagnostic methods for detection of Echinococcus granulosus infection, the causative agent of cystic echinococcosis in human and domestic animals. Two recombinant proteins EgAg5-38 sub-unit and P-29 of E. granulosus were expressed in prokaryotic expression vectors. The diagnostic potential of these two recombinant proteins was evaluated in the detection of cystic echinococcosis in buffaloes in IgG-ELISA. The
EgAg5-38 sub-unit and P-29 recombinant protein reacted fairly with the hydatid infected buffaloes with EgAg5-38 sub-unit showing sensitivity and specificity of 67.9 and 78.8%, respectively. However, the recombinant P-29 protein showed a higher sensitivity of 82.1% but a low specificity of 43.9% in the detection of hydatid infection in buffaloes. Cross-reactivity of these recombinant antigens in buffaloes naturally infected with several gastrointestinal trematodes and Sarcocystis was studied. The results showed that both these antigens cross-reacted with most of these parasites of buffaloes. Present study is the first report on the evaluation of diagnostic potential of E. granulosus recombinant EgAg5-38 sub-unit and P-29 antigens in buffaloes.

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