Evaluation of bio-engineered corneal scaffold for the repair of corneal defect in rabbit model

P SANGEETHA; S K MAITI; PANKAJ GAUTAM; KIRANJEET SINGH; ASWATHY GOPINATHAN; K P SINGH; DIVYA MOHAN; A R NINU; NAVEEN KUMAR

doi:10.56093/ijans.v87i11.75864

Authors

P SANGEETHA ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
S K MAITI ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
PANKAJ GAUTAM ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
KIRANJEET SINGH ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
ASWATHY GOPINATHAN ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
K P SINGH ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
DIVYA MOHAN ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
A R NINU ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India
NAVEEN KUMAR ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India

https://doi.org/10.56093/ijans.v87i11.75864

Keywords:

Antioxidants, Artemisia indica Willd, In vitro radical scavenging assays, Plant extracts

Abstract

Clinically healthy adult New Zealand white rabbits (27) of either sex, were randomly divided into three groups (A, B and C) having 9 animals each. Porcine cornea was made acellular by treating it with 1% sodium dodecyl sulphate (SDS). Rabbit bone marrow derived mesenchymal stem cells were seeded over this acellular matrix. A 5mm diameter lamellar keratectomy wound was created over the peripheral cornea of rabbits in all the 3 groups. In group A, the corneal defect was managed by simple tarsorrhaphy without any graft and is treated as control. In group B, defect was repaired with decellularized porcine cornea and in group C, corneal defect was repaired with r-MSC seeded decellularized cornea. On the basis of clinical, pathological and scanning electron microscopic examinations, mesenchymal stem cell seeded corneal scaffold showed better healing and vision when compared to
nonseeded scaffolds. Cell seeded corneal matrix was found to be an alternative to conventional means of surgical management of corneal ulcer.

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