Cryoprotective effect of low-density lipoproteins on post thaw semen quality in Hariana bull

MUKUL ANAND; SARVAJEET YADAV; VIJAY SINGH; SHALINI VASWANI; PANKAJ K SHUKLA

doi:10.56093/ijans.v87i11.75874

Authors

MUKUL ANAND DUVASU, Mathura, Uttar Pradesh 281 001 India
SARVAJEET YADAV DUVASU, Mathura, Uttar Pradesh 281 001 India
VIJAY SINGH DUVASU, Mathura, Uttar Pradesh 281 001 India
SHALINI VASWANI DUVASU, Mathura, Uttar Pradesh 281 001 India
PANKAJ K SHUKLA DUVASU, Mathura, Uttar Pradesh 281 001 India

https://doi.org/10.56093/ijans.v87i11.75874

Keywords:

CASA, Cryopreservation, Hariana bull, Low-density lipoproteins, Sperm characteristics

Abstract

The study aimed to evaluate the cryoprotective effect of low-density lipoprotein in Hariana bull semen. Four adult Hariana bull (3-4 years) after thorough andrological examination were selected for experiment. Semen was collected from each bull twice a week; total of 32 ejaculates (8 from each bull) were collected during the experiment. Collected sample from each bull was divided into 5 equal aliquots. Four aliquots were diluted separately with a Tris-based extender containing different concentration of low-density lipoproteins viz. 7% (T-1), 8% (T-2), 9% (T-3) and 10% (T- 4) as treatment while routinely used extender containing 20% hen egg yolk was used as control (C) to achieve final concentration of 125 million spermatozoa/ml. The diluted samples were cryopreserved and evaluated after 7 days. A significantly higher value of percent live sperms, sperm positive for hypo osmotic swelling test, sperms with intact acrosome and sperms exhibiting pattern F (uncapacitated sperm) were significantly higher in T-3 as compared to other treatment groups and control. The kinematic characters exhibited by sperm i.e forward progression %, VCL (μm/sec), VSL (μm/sec), VAP (μm/sec), Lin (%), Str (%), BCF (Hz) and ALH (μm) were significantly higher in T-3 as compared to other treatment group and control. In conclusion, extender containing 9% low density lipoproteins has better capacity to maintain the sperm character in post thaw Hariana bull semen and can be a preferred concentration to be utilized for cryopreservation with improved conception rate.

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